C6orf136 (NM_001161376) Human Tagged ORF Clone

CAT#: RG228412

C6orf136 (tGFP-tagged) - Human chromosome 6 open reading frame 136 (C6orf136), transcript variant 3

Product Images

Circular map for RG228412

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C6orf136
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228412 representing NM_001161376
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCAGCCCAGCCGGGGTGCGGCCCGGCGTCTCGGCCCTTGCCTGCGCGCCTACCAGGCTCGACCCC
  AGGTGAGCGGAGGAGAAGAGGGAGGGAGGAGAGGGGGCGGGGAGAGACCCTCCTCAAAGCCGGTGCGTGG
  GGCGGAGCGCGCGCTGGGTTCCGCGCAGGCGCAGAGACACCCGCCGCCCCTTCCCACCTGTGCCCTGCAG
  CGCGTGGACAGGCTAGGGGTCGCGGGAGCGGGAGGGAGGCGCTGCCGGGCCTGTCGCGCAAGGACGTCGG
  TCCTCCCAGGTTTGAGGGCGGTCAGGCGGGGTCAAGGCCAGGCAGCGGGGCGCGTCTGCGTTGCGCCCGA
  CTCTCCGCGGTTACCTGTGCCTAGAGGTGATTTGAAGGGCAGGGGCCGAGAGATTCGTAGCCCTGCTGCG
  GCGCCGTCCCGGAGTTCCCCGGCCCAGACCAGACCCGCGGGGCGCCCTCAGCAGCCCGCCCGTCTTGCAC
  TCGGAGAGCGGTCCTGGCAGGAAGGCCGGCCAGTGTGCACCCGGTTCGGGCCCCTGCGCCCGGGCTGGCA
  AGATGGCCACGCCCCCAGCAGAGACGGCGCCTCTAGGACACCATCGGGGACCGAGGACCAGCTTTATCCA
  GGGACTCTACCATTCCCACCCCTTTGGCCCCACTCCACGACAACCACTTCCCCATCTTCTCCTCTATTCT
  GGTCTCCCCTGCCCCCACGCCTTCCCACCCAGCGTCTTCCCCAGGTTCCCCCACTACCTCTCCCTCAGAT
  CCAGGCCCTCAGCTCAGCATGGGTGGTTCTCCCTCCAGGAAAGGGGGAGGAGGGACCAGGACCTGAGTTG
  CATAGCGGCTGCCTGGATGGGCTTAGAAGCCTTTTTGAGGGACCTCCCTGCCCCTATCCTGGGGCTTGGA
  TACCTTTCCAAGTCCCTGGAACTGCCCACCCTTCCCCTGCCACCCCGTCAGGAGATCCTAGTATGGAGGA
  ACATCTGTCTGTCATGTATGAGAGACTGAGACAAGAGCTTCCCAAGCTCTTCCTTCAGTCCCACGACTAC
  AGTCTGTATTCCTTGGATGTGGAATTCATCAATGAGATCCTCAACATACGTACCAAGGGCCGGACATGGT
  ACATTCTTTCACTGACCCTCTGCCGTTTCCTGGCCTGGAATTATTTTGCACACCTTCGTTTGGAGGTTTT
  ACAGCTGACCCGCCACCCTGAGAACTGGACCCTGCAAGCCCGGTGGCGGCTTGTGGGGCTGCCCGTCCAC
  TTGCTCTTTTTGCGGTTCTACAAGCGTGACAAAGACGAGCATTACCGGACCTATGATGCCTACTCCACTT
  TCTACCTGAATTCCAGTGGCCTCATTTGTCGCCATCGTCTAGATAAACTGATGCCTTCACACTCACCTCC
  AACGCCTGTGAAGAAGCTGCTAGTGGGAGCCCTGGTGGCCCTGGGGCTGTCAGAGCCAGAACCTGACTTA
  AACCTGTGTTCCAAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228412 representing NM_001161376
  Red=Cloning site Green=Tags(s)
  
  MYQPSRGAARRLGPCLRAYQARPQVSGGEEGGRRGGGERPSSKPVRGAERALGSAQAQRHPPPLPTCALQ
  RVDRLGVAGAGGRRCRACRARTSVLPGLRAVRRGQGQAAGRVCVAPDSPRLPVPRGDLKGRGREIRSPAA
  APSRSSPAQTRPAGRPQQPARLALGERSWQEGRPVCTRFGPLRPGWQDGHAPSRDGASRTPSGTEDQLYP
  GTLPFPPLWPHSTTTTSPSSPLFWSPLPPRLPTQRLPQVPPLPLPQIQALSSAWVVLPPGKGEEGPGPEL
  HSGCLDGLRSLFEGPPCPYPGAWIPFQVPGTAHPSPATPSGDPSMEEHLSVMYERLRQELPKLFLQSHDY
  SLYSLDVEFINEILNIRTKGRTWYILSLTLCRFLAWNYFAHLRLEVLQLTRHPENWTLQARWRLVGLPVH
  LLFLRFYKRDKDEHYRTYDAYSTFYLNSSGLICRHRLDKLMPSHSPPTPVKKLLVGALVALGLSEPEPDL
  NLCSKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161376
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161376.2
• RefSeq Size: 1984 bp
• RefSeq ORF: 1491 bp
• Locus ID: 221545
• Cytogenetics: 6p21.33