ALS2CR1 (NIF3L1) (NM_001136039) Human Tagged ORF Clone

CAT#: RG227762

NIF3L1 (tGFP-tagged) - Human NIF3 NGG1 interacting factor 3-like 1 (S. pombe) (NIF3L1), transcript variant 1

Product Images

Circular map for RG227762

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALS2CR1
• Synonyms: ALS2CR1; CALS-7; MDS015
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227762 representing NM_001136039
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCATCTTGCGTACGCCCAGTCCCCACGACAGTCCGGTTTGTAGATTCCCTGATCTGCAATTCTT
  CCCGTTCCTTCATGGATTTGAAGGCTCTCCTTTCTTCCTTGAATGACTTTGCATCCCTCTCGTTTGCTGA
  GAGTTGGGACAATGTTGGATTACTGGTGGAACCAAGCCCACCACATACTGTAAATACACTCTTCCTGACC
  AATGACCTGACTGAGGAAGTGATGGAGGAGGTGCTGCAAAAGAAGGCAGACCTCATTCTCTCCTACCATC
  CGCCTATCTTCCGACCCATGAAGCGCATAACCTGGAACACATGGAAGGAGCGCCTGGTGATCCGGGCTCT
  GGAGAACAGAGTCGGTATCTACTCTCCTCATACAGCCTATGATGCTGCGCCCCAGGGCGTCAACAACTGG
  TTGGCTAAAGGGCTTGGAGCTTGTACCTCCAGGCCCATACATCCTTCCAAAGCTCCCAACTACCCTACAG
  AGGGAAACCACCGAGTAGAATTCAACGTTAACTACACCCAAGACCTGGACAAAGTCATGTCTGCAGTGAA
  AGGAATTGACGGTGTTTCTGTCACTTCTTTTTCTGCTAGGACTGGTAATGAGGAACAAACACGGATTAAT
  CTGAATTGTACTCAGAAGGCTTTGATGCAGGTGGTAGATTTTCTTTCCCGGAACAAACAACTTTATCAGA
  AGACGGAAATTCTGTCACTGGAGAAGCCTTTGCTTCTACATACTGGAATGGGACGGTTATGCACACTGGA
  TGAATCTGTCTCCCTGGCAACCATGATTGATCGAATAAAAAGACACCTAAAACTATCTCATATTCGCTTA
  GCCCTTGGGGTGGGGAGAACCTTAGAGTCTCAAGTCAAAGTCGTGGCCCTGTGTGCTGGTTCTGGGAGCA
  GCGTTCTGCAGGGTGTTGAGGCTGACCTTTACCTCACAGGTGAGATGTCCCATCATGATACTTTGGATGC
  TGCTTCCCAAGGAATAAATGTCATCCTCTGTGAACACAGCAACACTGAACGAGGCTTTCTTTCTGACCTT
  CGAGATATGCTGGATTCTCACTTGGAGAATAAGATAAATATTATCCTATCAGAGACTGACAGGGACCCTC
  TTCAGGTGGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227762 representing NM_001136039
  Red=Cloning site Green=Tags(s)
  
  MLSSCVRPVPTTVRFVDSLICNSSRSFMDLKALLSSLNDFASLSFAESWDNVGLLVEPSPPHTVNTLFLT
  NDLTEEVMEEVLQKKADLILSYHPPIFRPMKRITWNTWKERLVIRALENRVGIYSPHTAYDAAPQGVNNW
  LAKGLGACTSRPIHPSKAPNYPTEGNHRVEFNVNYTQDLDKVMSAVKGIDGVSVTSFSARTGNEEQTRIN
  LNCTQKALMQVVDFLSRNKQLYQKTEILSLEKPLLLHTGMGRLCTLDESVSLATMIDRIKRHLKLSHIRL
  ALGVGRTLESQVKVVALCAGSGSSVLQGVEADLYLTGEMSHHDTLDAASQGINVILCEHSNTERGFLSDL
  RDMLDSHLENKINIILSETDRDPLQVV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136039
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136039.2, NP_001129511.1
• RefSeq Size: 1497 bp
• RefSeq ORF: 1134 bp
• Locus ID: 60491
• UniProt ID: Q9GZT8
• Cytogenetics: 2q33.1
• Gene Summary: May function as a transcriptional corepressor through its interaction with COPS2, negatively regulating the expression of genes involved in neuronal differentiation.[UniProtKB/Swiss-Prot Function]