ZNF447 (ZSCAN18) (NM_001145542) Human Tagged ORF Clone

CAT#: RG227481

ZSCAN18 (tGFP-tagged) - Human zinc finger and SCAN domain containing 18 (ZSCAN18), transcript variant 1

Product Images

Circular map for RG227481

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF447
• Synonyms: ZNF447
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227481 representing NM_001145542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAGAGTCGGAGGGCGAGCTGACGAGGACGCTACGGCGGCGGGAAGTCTGTGGGTTCTGGCACCAC
  CTGAGCCCACTGGGCATCTGGTCATCCCTGGCACCTCTCCTTTGGAGCCACCTTGTCCCTGGCTAGACAG
  TCACATTTTCCAGTGCCGTTTTGGAAAGATGTTGCCTTTGGAGAAGGCGTTTGCCTCCCCCAGGAGCTCC
  CCAGCCCCGCCGGATCTGCCCACGCCGGGGTCAGCAGCCGGAGTCCAGCAGGAAGAACCCGAGACCATCC
  CTGAGAGGACCCCTGCTGACCTGGAGTTCTCCCGCCTGCGTTTCCGGGAATTTGTCTACCAGGAGGCTGC
  CGGGCCCCACCAGACCCTGGCCCGGCTGCATGAGCTGTGCCGCCAGTGGCTGATGCCTGAGGCGCGCTCC
  AAGGAGCAGATGCTGGAGCTGCTGGTGCTGGAGCAGTTCCTGGGCATCCTGCCTGATAAGGTCCGGCCCT
  GGGTGGTGGCACAGTACCCTGAGAGCTGCAAGAAGGCAGCCTCCCTGGTGGAGGGCCTCGCTGATGTCCT
  GGAAGAGCCAGGGATGCTGCTGGGCTCCCCTGCGGGCTCATCCTCAATTCTTAGCGATGGAGTGTACGAG
  AGGCACATGGACCCTCTGCTGCTACCAGGCGAGCTCGCGAGCCCCAGCCAGGCCCTTGGAGCTGGGGAGA
  TCCCGGCACCTTCTGAGACACCCTGGCTTTCTCCGGACCCCCTGTTTCTGGAACAGAGGAGGGTCAGAGA
  AGCAAAGACCGAAGAGGACGGCCCTGCCAACACCGAGCAGAAGCTGAAGTCCTTTCCAGAGGACCCTCAG
  CACCTGGGGGAGTGGGGCCACCTGGACCCTGCCGAGGAGAACCTGAAGAGCTACCGGAAGCTGCTCCTGT
  GGGGGTATCAGCTTTCCCAGCCTGACGCTGCCTCCAGGCTGGACACTGAGGAACTCCGGTTGGTGGAAAG
  AGATCCACAAGGAAGCAGCCTCCCAGAAGGCGGGAGGCGGCAGGAGAGCGCTGGGTGCGCCTGCGAGGAG
  GCCGCCCCCGCGGGGGTGCTGCCTGAGCTGCCTACGGAGGCGCCCCCTGGGGACGCCCTTGCCGATCCCC
  CGTCGGGCACCACTGAGGAGGAGGAAGAGCAGCCTGGGAAGGCCCCGGACCCGCAGGACCCCCAGGACGC
  GGAGTCCGACTCTGCCACCGGATCGCAGAGGCAGTCCGTCATCCAGCAGCCTGCCCCGGACAGGGGCACG
  GCGAAACTGGGAACCAAGAGGCCGCACCCCGAGGATGGGGACGGGCAGAGCCTCGAGGGCGTCTCTAGCT
  CCGGCGACAGCGCAGGGCTGGAGGCCGGGCAGGGCCCTGGGGCTGACGAGCCGGGCTTGTCCCGCGGGAA
  GCCCTATGCCTGCGGCGAGTGCGGGGAGGCCTTCGCGTGGCTCTCGCACCTGATGGAGCACCACAGCAGC
  CATGGCGGCCGGAAGCGCTACGCCTGTCAGGGCTGCTGGAAGACCTTCCACTTCAGCCTGGCCCTAGCCG
  AGCACCAGAAGACCCACGAGAAGGAGAAAAGCTACGCGCTGGGGGGCGCCCGGGGCCCCCAACCGTCCAC
  CCGCGAAGCCCAGGCGGGGGCTAGGGCGGGCGGTCCCCCAGAGAGCGTGGAGGGCGAGGCTCCCCCCGCA
  CCCCCAGAGGCGCAGAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227481 representing NM_001145542
  Red=Cloning site Green=Tags(s)
  
  MRRVGGRADEDATAAGSLWVLAPPEPTGHLVIPGTSPLEPPCPWLDSHIFQCRFGKMLPLEKAFASPRSS
  PAPPDLPTPGSAAGVQQEEPETIPERTPADLEFSRLRFREFVYQEAAGPHQTLARLHELCRQWLMPEARS
  KEQMLELLVLEQFLGILPDKVRPWVVAQYPESCKKAASLVEGLADVLEEPGMLLGSPAGSSSILSDGVYE
  RHMDPLLLPGELASPSQALGAGEIPAPSETPWLSPDPLFLEQRRVREAKTEEDGPANTEQKLKSFPEDPQ
  HLGEWGHLDPAEENLKSYRKLLLWGYQLSQPDAASRLDTEELRLVERDPQGSSLPEGGRRQESAGCACEE
  AAPAGVLPELPTEAPPGDALADPPSGTTEEEEEQPGKAPDPQDPQDAESDSATGSQRQSVIQQPAPDRGT
  AKLGTKRPHPEDGDGQSLEGVSSSGDSAGLEAGQGPGADEPGLSRGKPYACGECGEAFAWLSHLMEHHSS
  HGGRKRYACQGCWKTFHFSLALAEHQKTHEKEKSYALGGARGPQPSTREAQAGARAGGPPESVEGEAPPA
  PPEAQR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145542
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145542.1, NP_001139014.1
• RefSeq Size: 2605 bp
• RefSeq ORF: 1701 bp
• Locus ID: 65982
• UniProt ID: Q8TBC5
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]