LRRC8B (NM_001134476) Human Tagged ORF Clone

CAT#: RG226175

LRRC8B (tGFP-tagged) - Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 2

Product Images

Circular map for RG226175

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LRRC8B
• Synonyms: TA-LRRP; TALRRP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226175 representing NM_001134476.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATTACACTAACTGAGCTAAAATGCTTAGCAGATGCTCAGTCATCTTATCACATCTTAAAACCATGG
  TGGGACGTCTTCTGGTATTACATCACACTGATCATGCTGCTGGTGGCCGTGCTGGCCGGAGCTCTCCAG
  CTGACGCAGAGCAGGGTTCTGTGCTGTCTTCCATGCAAAGTGGAATTTGACAATCACTGTGCCGTGCCT
  TGGGACATCCTGAAAGCCAGCATGAACACATCCTCTAATCCTGGGACACCGCTTCCGCTCCCCCTCCGA
  ATTCAGAATGACCTCCACCGACAGCAGTACTCCTATATTGATGCCGTCTGTTACGAGAAACAGCTCCAT
  TGGTTTGCAAAGTTTTTCCCCTATCTGGTGCTCTTGCACACGCTCATCTTTGCAGCCTGCAGCAACTTT
  TGGCTTCACTACCCCAGTACCAGTTCCAGGCTCGAGCATTTTGTGGCCATCCTTCACAAGTGCTTCGAT
  TCTCCATGGACCACCCGCGCCCTTTCAGAAACAGTGGCTGAGCAGTCAGTGAGGCCTCTGAAACTCTCC
  AAGTCCAAGATTTTGCTTTCGTCCTCAGGGTGTTCAGCTGACATAGATTCCGGCAAACAGTCATTGCCC
  TACCCACAGCCAGGTTTGGAGTCAGCTGGCATAGAAAGCCCAACTTCCAGTGTCCTGGACAAGAAGGAG
  GGTGAACAGGCCAAAGCCATCTTTGAAAAAGTGAAAAGATTCCGCATGCATGTGGAGCAGAAGGACATC
  ATTTATAGAGTATATCTGAAACAGATAATAGTCAAAGTCATTTTGTTTGTGCTCATCATAACTTATGTT
  CCATATTTTTTAACCCACATCACTCTTGAAATCGACTGTTCAGTTGATGTGCAGGCTTTTACAGGATAT
  AAGCGCTACCAGTGTGTCTATTCCTTGGCAGAAATCTTTAAGGTCCTGGCTTCATTTTATGTCATTTTG
  GTTATACTTTATGGTCTGACCTCTTCCTACAGCCTGTGGTGGATGCTGAGGAGTTCCCTGAAGCAATAT
  TCCTTTGAGGCGTTAAGAGAAAAAAGCAACTACAGTGACATCCCTGATGTCAAGAATGACTTTGCCTTC
  ATCCTTCATCTGGCTGATCAGTATGATCCTCTTTATTCCAAACGCTTCTCCATATTCCTATCAGAGGTC
  AGTGAGAACAAACTGAAACAGATCAACCTCAATAATGAATGGACAGTTGAGAAACTGAAAAGTAAGCTT
  GTGAAAAATGCCCAGGACAAGATAGAACTGCATCTTTTTATGCTCAACGGTCTTCCAGACAATGTCTTT
  GAGTTAACTGAAATGGAAGTGCTAAGCCTGGAGCTTATCCCAGAGGTGAAGCTGCCCTCTGCAGTCTCA
  CAGCTGGTCAACCTCAAGGAGCTTCGTGTGTACCATTCATCTCTGGTCGTAGACCATCCTGCACTGGCC
  TTTCTAGAGGAGAATTTAAAAATCCTCCGCCTGAAATTTACTGAAATGGGAAAAATCCCACGCTGGGTA
  TTTCACCTCAAGAATCTCAAGGAACTTTATCTTTCGGGCTGTGTTCTCCCTGAACAGTTGAGTACTATG
  CAGTTGGAGGGCTTTCAGGACTTAAAAAATCTAAGGACCCTGTACTTGAAGAGCAGCCTCTCCCGGATC
  CCACAAGTTGTTACAGACCTCCTGCCTTCATTGCAGAAACTGTCCCTTGATAATGAGGGAAGCAAACTG
  GTTGTGTTGAACAACTTGAAAAAGATGGTCAATCTGAAAAGCCTAGAACTGATCAGCTGTGACCTGGAA
  CGCATCCCACATTCCATTTTCAGCCTGAATAATTTGCATGAGTTAGACCTAAGGGAAAATAACCTTAAA
  ACTGTGGAAGAGATCATTAGCTTTCAGCATCTTCAGAATCTTTCCTGCTTAAAGTTGTGGCACAATAAC
  ATTGCTTATATTCCTGCACAGATTGGGGCATTATCTAACCTAGAGCAGCTCTCTTTGGACCATAATAAT
  ATTGAGAATCTGCCCTTGCAGCTTTTCCTATGCACTAAACTACATTATTTGGATCTAAGCTATAACCAC
  TTGACCTTCATTCCAGAAGAAATCCAGTATCTGAGTAATTTGCAGTACTTTGCTGTGACCAACAACAAT
  ATTGAGATGCTACCAGATGGGCTGTTTCAGTGCAAAAAGCTGCAGTGTTTACTTTTGGGGAAAAATAGC
  TTGATGAATTTGTCCCCTCATGTGGGTGAGCTGTCAAACCTTACTCATCTGGAGCTCATTGGTAATTAC
  CTGGAAACACTTCCTCCTGAACTAGAAGGATGTCAGTCCCTAAAACGGAACTGTCTGATTGTTGAGGAG
  AACTTGCTCAATACTCTTCCTCTCCCTGTAACAGAACGTTTACAGACGTGCTTAGACAAATGT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG226175
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNHCAVP
  WDILKASMNTSSNPGTPLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNF
  WLHYPSTSSRLEHFVAILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKILLSSSGCSADIDSGKQSLP
  YPQPGLESAGIESPTSSVLDKKEGEQAKAIFEKVKRFRMHVEQKDIIYRVYLKQIIVKVILFVLIITYV
  PYFLTHITLEIDCSVDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVILYGLTSSYSLWWMLRSSLKQY
  SFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVEKLKSKL
  VKNAQDKIELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPSAVSQLVNLKELRVYHSSLVVDHPALA
  FLEENLKILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSTMQLEGFQDLKNLRTLYLKSSLSRI
  PQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELISCDLERIPHSIFSLNNLHELDLRENNLK
  TVEEIISFQHLQNLSCLKLWHNNIAYIPAQIGALSNLEQLSLDHNNIENLPLQLFLCTKLHYLDLSYNH
  LTFIPEEIQYLSNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGKNSLMNLSPHVGELSNLTHLELIGNY
  LETLPPELEGCQSLKRNCLIVEENLLNTLPLPVTERLQTCLDKC
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134476
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 7766 bp
• RefSeq ORF: 2412 bp
• Locus ID: 23507
• UniProt ID: Q6P9F7
• Cytogenetics: 1p22.2
• Protein Families: Transmembrane
• MW: 92.4 kDa
• Gene Summary: Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26824658, PubMed:28193731). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658, PubMed:28193731).[UniProtKB/Swiss-Prot Function]