TMEM201 (NM_001130924) Human Tagged ORF Clone

CAT#: RG226053

TMEM201 (tGFP-tagged) - Human transmembrane protein 201 (TMEM201), transcript variant 1

Product Images

Circular map for RG226053

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMEM201
• Synonyms: Ima1; NET5; SAMP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226053 representing NM_001130924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGAGTGAGCGCGCTGCTGGCCCGCTGCCCCACGGCCGGCCTGGCCGGCGGCCTGGGGGTCACGG
  CGTGCGCCGCGGCCGGCGTGTTGCTCTACCGGATCGCGCGGAGGATGAAGCCAACGCACACGATGGTCAA
  CTGCTGGTTCTGCAACCAGGATACGCTGGTGCCCTATGGGAACCGCAACTGCTGGGACTGTCCCCACTGC
  GAGCAGTACAACGGCTTCCAGGAGAACGGCGACTACAACAAGCCGATCCCCGCCCAGTACTTGGAGCACC
  TGAACCACGTGGTGAGCAGCGCGCCCAGCCTGCGCGACCCTTCGCAGCCGCAGCAGTGGGTGAGCAGCCA
  AGTCCTGCTGTGCAAGAGGTGCAACCACCACCAGACCACCAAGATCAAGCAGCTGGCCGCCTTCGCTCCC
  CGCGAGGAGGGCAGGTATGACGAGGAGGTCGAGGTGTACCGGCATCACCTGGAGCAGATGTACAAGCTGT
  GCCGGCCGTGCCAAGCGGCTGTGGAGTACTACATCAAGCACCAGAACCGCCAGCTGCGCGCCCTGTTGCT
  CAGCCACCAGTTCAAGCGCCGGGAGGCCGACCAGACCCACGCACAGAACTTCTCCTCCGCCGTGAAGTCC
  CCGGTCCAGGTCATCCTGCTCCGTGCCCTCGCCTTCCTGGCCTGCGCCTTCCTACTGACCACCGCGCTGT
  ATGGGGCCAGCGGACACTTCGCCCCAGGCACCACTGTGCCCCTGGCCCTGCCACCTGGTGGCAATGGCTC
  AGCCACACCTGACAATGGCACCACCCCTGGGGCCGAGGGCTGGCGGCAGTTGCTGGGCCTACTCCCCGAG
  CACATGGCGGAGAAGCTGTGTGAGGCCTGGGCCTTTGGGCAGAGCCACCAGACGGGCGTCGTGGCACTGG
  GCCTACTCACCTGCCTGCTGGCAATGCTGCTGGCTGGCCGCATCAGGCTCCGGAGGATCGATGCCTTCTG
  CACCTGCCTGTGGGCCCTGCTGCTGGGGCTGCACCTGGCTGAGCAGCACCTGCAGGCCGCCTCGCCTAGC
  TGGCTAGACACGCTCAAGTTCAGCACCACATCTTTGTGCTGCCTGGTTGGCTTCACGGCGGCTGTGGCCA
  CAAGGAAGGCAACGGGCCCACGGAGGTTCCGGCCCCGAAGGTTCTTCCCAGGAGACTCTGCCGGCCTTTT
  CCCCACCAGCCCCAGCTTGGCCATCCCTCACCCGAGTGTCGGAGGCTCTCCAGCGTCTCTGTTCATCCCC
  AGCCCGCCCAGCTTCCTGCCCCTCGCCAACCAGCAGCTCTTCCGGTCTCCTCGACGGACCTCACCCTCCT
  CATTGCCTGGCCGCCTCAGCCGGGCCCTCTCTCTGGGAACCATACCCTCTCTGACTCGAGCAGATTACTT
  CTCTCTTCTGTCGGGGAGCTGCCCCTCCTCCCCACTCCCTTCCCCAGCGCCTTCCGTGGCCGGCTCGGTG
  GCCTCCAGCTCCGGCTCTCTGCGCCACCGCAGGCCCCTCATCAGCCCTGCCCGGCTCAACCTGAAGGGAC
  AGAAGCTGCTGCTGTTCCCGTCACCCCCTGGAGAGGCCCCCACCACGCCCAGCAGCTCCGATGAGCACTC
  GCCTCACAACGGCAGCCTCTTCACCATGGAGCCGCCCCATGTTCCCCGGAAGCCGCCCCTGCAGGACGTG
  AAGCACGCCCTGGACCTGAGATCCAAGCTGGAAAGAGGCAGTGCCTGCAGCAACCGCTCCATCAAGAAAG
  AGGACGACTCTTCCCAGTCATCTACCTGTGTGGTGGACACCACCACCAGGGGCTGCTCGGAGGAGGCCGC
  CACCTGGAGAGGTCGTTTCGGCCCTTCCCTGGTCCGGGGCCTCCTGGCCGTGAGCTTGGCCGCCAACGCC
  CTGTTCACCTCGGTGTTTCTGTACCAGAGCCTGCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226053 representing NM_001130924
  Red=Cloning site Green=Tags(s)
  
  MEGVSALLARCPTAGLAGGLGVTACAAAGVLLYRIARRMKPTHTMVNCWFCNQDTLVPYGNRNCWDCPHC
  EQYNGFQENGDYNKPIPAQYLEHLNHVVSSAPSLRDPSQPQQWVSSQVLLCKRCNHHQTTKIKQLAAFAP
  REEGRYDEEVEVYRHHLEQMYKLCRPCQAAVEYYIKHQNRQLRALLLSHQFKRREADQTHAQNFSSAVKS
  PVQVILLRALAFLACAFLLTTALYGASGHFAPGTTVPLALPPGGNGSATPDNGTTPGAEGWRQLLGLLPE
  HMAEKLCEAWAFGQSHQTGVVALGLLTCLLAMLLAGRIRLRRIDAFCTCLWALLLGLHLAEQHLQAASPS
  WLDTLKFSTTSLCCLVGFTAAVATRKATGPRRFRPRRFFPGDSAGLFPTSPSLAIPHPSVGGSPASLFIP
  SPPSFLPLANQQLFRSPRRTSPSSLPGRLSRALSLGTIPSLTRADYFSLLSGSCPSSPLPSPAPSVAGSV
  ASSSGSLRHRRPLISPARLNLKGQKLLLFPSPPGEAPTTPSSSDEHSPHNGSLFTMEPPHVPRKPPLQDV
  KHALDLRSKLERGSACSNRSIKKEDDSSQSSTCVVDTTTRGCSEEAATWRGRFGPSLVRGLLAVSLAANA
  LFTSVFLYQSLR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130924
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130924.1, NP_001124396.1
• RefSeq Size: 3740 bp
• RefSeq ORF: 2001 bp
• Locus ID: 199953
• UniProt ID: Q5SNT2
• Cytogenetics: 1p36.22
• Protein Families: Transmembrane
• Gene Summary: Involved in nuclear movement during fibroblast polarization and migration. Proposed to be involved in actin-dependent nuclear movement via association with transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow (By similarity). Overexpression can recruit Ran GTPase to the nuclear periphery (PubMed:27541860).[UniProtKB/Swiss-Prot Function]