FIP1L1 (NM_001134938) Human Tagged ORF Clone

CAT#: RG225893

FIP1L1 (tGFP-tagged) - Human FIP1 like 1 (S. cerevisiae) (FIP1L1), transcript variant 3

Product Images

Circular map for RG225893

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FIP1L1
• Synonyms: FIP1; hFip1; Rhe
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225893 representing NM_001134938
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGGCGAGGTCGAGCGCCTAGTGTCGGAGCTGAGCGGCGGGACCGGAGGGGATGAGGAGGAAG
  AGTGGCTCTATGGCGATGAAAATGAAGTTGAAAGGCCAGAAGAAGAAAATGCCAGTGCTAATCCTCCATC
  TGGAATTGAAGATGAAACTGCTGAAAATGGTGTACCAAAACCGAAAGTGACTGAGACCGAAGATGATAGT
  GATAGTGACAGCGATGATGATGAAGATGATGTTCATGTCACTATAGGAGACATTAAAACGGGAGCACCAC
  AGTATGGGAGTTATGGTACAGCACCTGTAAATCTTAACATCAAGACAGGGGGAAGAGTTTATGGAACTAC
  AGGGACAAAAGTCAAAGGAGTAGACCTTGATGCACCTGGAAGCATTAATGGAGTTCCACTCTTAGAGGTA
  GATTTGGATTCTTTTGAAGATAAACCATGGCGTAAACCTGGTGCTGATCTTTCTGATTATTTTAATTATG
  GGTTTAATGAAGATACCTGGAAAGCTTACTGTGAAAAACAAAAGAGGATACGAATGGGACTTGAAGTTAT
  ACCAGTAACCTCTACTACAAATAAAATTACGGTACAGCAGGGAAGAACTGGAAACTCAGAGAAAGAAACT
  GCCCTTCCATCTACAAAAGCTGAGTTTACTTCTCCTCCTTCTTTGTTCAAGACTGGGCTTCCACCGAGCA
  GGAGATTACCTGGGGCAATTGATGTTATCGGTCAGACTATAACTATCAGCCGAGTAGAAGGCAGGCGACG
  GGCAAATGAGAACAGCAACATACAGGTCCTTTCTGAAAGATCTGCTACTGAAGTAGACAACAATTTTAGC
  AAACCACCTCCGTTTTTCCCTCCAGGAGCTCCTCCCACTCACCTTCCACCTCCTCCATTTCTTCCACCTC
  CTCCGACTGTCAGCACTGCTCCACCTCTGATTCCACCACCGGGTTTTCCTCCTCCACCAGGCGCTCCACC
  TCCATCTCTTATACCAACAATAGAAAGTGGACATTCCTCTGGTTATGATAGTCGTTCTGCACGTGCATTT
  CCATATGGCAATGTTGCCTTTCCCCATCTTCCTGGTTCTGCTCCTTCGTGGCCTAGTCTTGTGGACACCA
  GCAAGCAGTGGGACTATTATGCCAGAAGAGAGAAAGACCGAGATAGAGAGAGAGACAGAGACAGAGAGCG
  AGACCGTGATCGGGACAGAGAAAGAGAACGCACCAGAGAGAGAGAGAGGGAGCGTGATCACAGTCCTACA
  CCAAGTGTTTTCAACAGCGATGAAGAACGATACAGATACAGGGAATATGCAGAAAGAGGTTATGAGCGTC
  ACAGAGCAAGTCGAGAAAAAGAAGAACGACATAGAGAAAGACGACACAGGGAGAAAGAGGAAACCAGACA
  TAAGTCTTCTCGAAGTAATAGTAGACGTCGCCATGAAAGTGAAGAAGGAGATAGTCACAGGAGACACAAA
  CACAAAAAATCTAAAAGAAGCAAAGAAGGAAAAGAAGCGGGCAGTGAGCCTGCCCCTGAACAGGAGAGCA
  CCGAAGCTACACCTGCAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225893 representing NM_001134938
  Red=Cloning site Green=Tags(s)
  
  MSAGEVERLVSELSGGTGGDEEEEWLYGDENEVERPEEENASANPPSGIEDETAENGVPKPKVTETEDDS
  DSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKTGGRVYGTTGTKVKGVDLDAPGSINGVPLLEV
  DLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRIRMGLEVIPVTSTTNKITVQQGRTGNSEKET
  ALPSTKAEFTSPPSLFKTGLPPSRRLPGAIDVIGQTITISRVEGRRRANENSNIQVLSERSATEVDNNFS
  KPPPFFPPGAPPTHLPPPPFLPPPPTVSTAPPLIPPPGFPPPPGAPPPSLIPTIESGHSSGYDSRSARAF
  PYGNVAFPHLPGSAPSWPSLVDTSKQWDYYARREKDRDRERDRDRERDRDRDRERERTRERERERDHSPT
  PSVFNSDEERYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHESEEGDSHRRHK
  HKKSKRSKEGKEAGSEPAPEQESTEATPAE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134938
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134938.2
• RefSeq Size: 2236 bp
• RefSeq ORF: 1563 bp
• Locus ID: 81608
• UniProt ID: Q6UN15
• Cytogenetics: 4q12
• Gene Summary: This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]