Nuclear Factor 1 (NFIA) (NM_001134673) Human Tagged ORF Clone

CAT#: RG225878

NFIA (tGFP-tagged) - Human nuclear factor I/A (NFIA), transcript variant 1

Product Images

Circular map for RG225878

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nuclear Factor 1
• Synonyms: BRMUTD; CTF; NF-I/A; NF1-A; NFI-A; NFI-L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225878 representing NM_001134673
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCTCCGCTCTGTCTCACCCAGGATGAATTTCATCCTTTCATCGAAGCACTTCTGCCCCACGTCC
  GAGCCTTTGCCTACACATGGTTCAACCTGCAGGCCCGAAAACGAAAATACTTCAAAAAACATGAAAAGCG
  TATGTCAAAAGAAGAAGAGAGAGCCGTGAAGGATGAATTGCTAAGTGAAAAACCAGAGGTCAAGCAGAAG
  TGGGCATCTCGACTTCTGGCAAAGTTGCGGAAAGATATCCGACCCGAATATCGAGAGGATTTTGTTCTTA
  CAGTTACAGGGAAAAAACCTCCATGTTGTGTTCTTTCCAACCCAGACCAGAAAGGCAAGATGCGAAGAAT
  TGACTGCCTCCGCCAGGCAGATAAAGTCTGGAGGTTGGACCTTGTTATGGTGATTTTGTTTAAAGGTATT
  CCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGTCCCCACAATGCTCTAATCCAGGGCTCTGTGTCC
  AACCCCATCACATAGGGGTTTCTGTTAAGGAACTCGATTTATATTTGGCATACTTTGTGCATGCAGCAGA
  TTCAAGTCAATCTGAAAGTCCCAGCCAGCCAAGTGACGCTGACATTAAGGACCAGCCAGAAAATGGACAT
  TTGGGCTTCCAGGACAGTTTTGTCACATCAGGTGTTTTTAGTGTCACTGAGCTAGTAAGAGTGTCACAGA
  CACCAATAGCTGCAGGAACTGGCCCAAATTTTTCTCTCTCAGATTTGGAAAGTTCTTCATACTACAGCAT
  GAGTCCAGGAGCAATGAGGAGGTCTTTACCCAGCACATCCTCTACGAGCTCCACAAAGCGCCTCAAGTCT
  GTGGAGGATGAAATGGACAGTCCTGGTGAGGAGCCATTTTATACAGGCCAAGGGCGCTCCCCAGGAAGTG
  GCAGTCAGTCAAGTGGATGGCATGAAGTGGAGCCAGGAATGCCATCTCCAACCACACTGAAGAAGTCGGA
  GAAGTCTGGTTTCAGCAGCCCCTCCCCTTCACAGACCTCCTCCCTGGGAACGGCGTTCACACAGCATCAC
  CGACCTGTCATTACAGGACCCAGAGCAAGTCCGCATGCAACACCATCGACTCTTCATTTCCCGACATCAC
  CCATTATCCAGCAGCCTGGGCCTTACTTCTCACACCCAGCCATCCGCTATCACCCTCAGGAGACGCTGAA
  AGAATTTGTCCAACTTGTCTGCCCTGATGCTGGTCAGCAGGCTGGACAGGTGGGGTTCCTCAATCCCAAT
  GGGAGCAGCCAAGGCAAGGTGCACAACCCATTCCTTCCCACCCCAATGTTGCCACCGCCACCGCCACCAC
  CGATGGCCAGGCCTGTGCCTCTGCCGGTGCCAGACACAAAGCCTCCAACCACGTCAACAGAAGGAGGTGC
  AGCCTCCCCCACGTCACCAACCTACTCGACACCCAGCACCTCCCCCGCAAACCGATTCGTCAGTGTTGGA
  CCACGGGATCCAAGCTTTGTAAATATCCCTCAACAGACACAGTCCTGGTACCTGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225878 representing NM_001134673
  Red=Cloning site Green=Tags(s)
  
  MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQK
  WASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGI
  PLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGH
  LGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKS
  VEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHH
  RPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPN
  GSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPTYSTPSTSPANRFVSVG
  PRDPSFVNIPQQTQSWYLG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134673
• ORF Size: 1527 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134673.4
• RefSeq Size: 9499 bp
• RefSeq ORF: 1530 bp
• Locus ID: 4774
• UniProt ID: Q12857
• Cytogenetics: 1p31.3
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]