TEDC1 (NM_001134876) Human Tagged ORF Clone

CAT#: RG225602

C14orf80 (tGFP-tagged) - Human chromosome 14 open reading frame 80 (C14orf80), transcript variant 2

Product Images

Circular map for RG225602

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TEDC1
• Synonyms: C14orf80
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225602 representing NM_001134876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGGCGGCGGCAGCGGGTGGACCCCGCGGCTGGGGCCCGGGCCGGGGCCCTGCCTGAGGCCATCG
  CCGCGTTGAGTCGGTCGCTGCCCTCGGGACCCAGCCCCGAGATCTTCCGCCGCGCCAAGTTCGACCGTCC
  GGAGGCGACCTCCGCGCTCTGGCAGCTCCTCTTCCGTGTGCTCTCGCCACTCCCTGCGGGCAACGCCTTG
  GCATCGCTCGCCCTGGAGGTCCAAGCCCGCTTGGTGAAGTCAGCACTATGCTCCCAGGGCTACCCGAGGC
  TGGCACTGGCACAACTACCTGAGGATGGCTCGCAGGGCAGTCGGGAGCTGCTGCTGGCTCTGTCCTGGCT
  CTTGGCCCGAGGACCTGTGCCCGAGCAGATGCTGGCCCAGGCCCGAGTGCCTCTGGGTGACGAGATGACT
  GTGTGCCAGTGTGAGGCCCTGGCCAGCCCTGGCCCACCTGCACCCCACATGGAAGCAGAGGGTCCTGTGG
  ATGTCCGCCATGTGCAGTGGCTGATGGGAAAGCTGCGGTTCCGGTGGCGCCAGCTGGTGTCCAGTCAGCA
  GGAGCAGTGCGCCCTCCTGAGCAAGCTGCTGCGGACTCTGGAGCGTGAGAACCAGCGCCTGGAGGCTGTC
  CTGGCGTGGCGGCGCTCTGAGCTGGTCTTCTGGCGGTGGATGGACACGGTCCTGGGCACCTGTGCCCCGG
  AGGTGCCTGCTGCAGCCTCACAGCCCACCTTCCTGCCCTGGGTCCCCGAGCGCGGGGGTGGCGAGTTGGA
  CCTGGTAGTGCGGGAGCTGCAGGCACTGGAGGAGGAGCTGCGGGAGGCTGCGGAGCGCAGGCGGGCGGCC
  TGGGAGGCCAAGGCTGGAGGCTGTGGACGGGGGCCAGAGTGGAGTGCCGCGCGGCGGGCCTCTCGGGAGG
  CTGTGGAAAAGGAGCTGGGAGCTCTACAGCAGTGCTGGGAGCGAGACGGTGGCCCGGCCCAGCCCCATGG
  GCCACACCGGCTGGTGAGACGAGAGGATGGGGCAGCAGGGGACCGGGACCTGCGGGCAGCTGTGGTGATC
  AGGACGCTGAGGAGCCAGGAGGCCTGCCTGGAGGCGGTGCTACGTCGACTACAGGGACAGTGTCGGCAGG
  AACTGGCCAGGCTGGTGGGAGCCCGCCCTGGTCTCATCTGGATCCCGCCACCTGGACGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225602 representing NM_001134876
  Red=Cloning site Green=Tags(s)
  
  MGRRRQRVDPAAGARAGALPEAIAALSRSLPSGPSPEIFRRAKFDRPEATSALWQLLFRVLSPLPAGNAL
  ASLALEVQARLVKSALCSQGYPRLALAQLPEDGSQGSRELLLALSWLLARGPVPEQMLAQARVPLGDEMT
  VCQCEALASPGPPAPHMEAEGPVDVRHVQWLMGKLRFRWRQLVSSQQEQCALLSKLLRTLERENQRLEAV
  LAWRRSELVFWRWMDTVLGTCAPEVPAAASQPTFLPWVPERGGGELDLVVRELQALEEELREAAERRRAA
  WEAKAGGCGRGPEWSAARRASREAVEKELGALQQCWERDGGPAQPHGPHRLVRREDGAAGDRDLRAAVVI
  RTLRSQEACLEAVLRRLQGQCRQELARLVGARPGLIWIPPPGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134876
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134876.1, NP_001128348.1
• RefSeq Size: 1620 bp
• RefSeq ORF: 1182 bp
• Locus ID: 283643
• UniProt ID: Q86SX3
• Cytogenetics: 14q32.33
• Gene Summary: May play a role in counteracting perturbation of actin filaments, such as after treatment with the actin depolymerizing microbial metabolite Chivosazole F.[UniProtKB/Swiss-Prot Function]