RNF90 (TRIM7) (NM_203293) Human Tagged ORF Clone

CAT#: RG224754

TRIM7 (tGFP-tagged) - Human tripartite motif containing 7 (TRIM7), transcript variant 1

Product Images

Circular map for RG224754

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RNF90
• Synonyms: GNIP; RNF90
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224754 representing NM_203293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGTGGGACCGCGGACCGGCCCCGGAACCGGCGCCGAGGCTCTAGCGCTGGCGGCAGAGCTGC
  AGGGCGAGGCGACGTGCTCCATCTGCCTAGAGCTCTTTCGTGAGCCGGTGTCCGTCGAGTGCGGCCACAG
  CTTCTGCCGCGCCTGCATAGGGCGCTGCTGGGAGCGCCCGGGCGCGGGGTCTGTTGGGGCCGCCACCCGC
  GCGCCCCCCTTCCCACTGCCCTGTCCGCAGTGCCGCGAGCCCGCGCGCCCCAGTCAGCTGCGGCCCAACC
  GGCAGCTGGCGGCAGTGGCCACGCTCCTGCGGCGCTTCAGCCTGCCCGCGGCTGCCCCGGGAGAGCACGG
  GTCTCAGGCGGCCGCGGCCCGGGCAGCGGCTGCCCGCTGCGGGCAGCATGGCGAACCCTTCAAGCTCTAC
  TGCCAGGACGACGGACGCGCCATCTGCGTGGTGTGCGACCGCGCCCGCGAGCACCGCGAGCACGCCGTGC
  TGCCGCTGGACGAGGCGGTGCAGGAGGCCAAGGAGCTCTTGGAGTCCAGGCTGAGGGTCTTGAAGAAGGA
  ACTGGAGGACTGTGAGGTGTTCCGGTCCACGGAAAAGAAGGAGAGCAAGGAGCTGCTGAAACAGATGGCA
  GCGGAGCAGGAGAAGGTGGGGGCAGAGTTCCAGGCACTGAGGGCTTTCCTGGTGGAGCAGGAGGGTCGGC
  TGCTAGGCCGCCTGGAGGAACTGTCCCGGGAGGTGGCACAGAAGCAGAATGAGAACCTGGCCCAGCTCGG
  GGTTGAGATCACCCAGCTGTCCAAGCTCAGCAGCCAGATCCAGGAGACAGCTCAAAAGCCTGACCTTGAC
  TTTCTCCAGGAATTCAAAAGCACGCTGAGCAGGTGTAGCAATGTGCCTGGCCCCAAGCCAACCACAGTCT
  CTTCTGAGATGAAGAATAAAGTCTGGAATGTTTCTCTCAAGACCTTTGTCTTAAAAGGGATGCTGAAGAA
  GTTCAAAGAGGACCTTCGGGGAGAGCTGGAGAAAGAGGAGAAAGTGGAGCTCACCTTGGATCCCGACACG
  GCCAACCCGCGCCTCATCCTCTCTCTGGATCTTAAGGGCGTGCGCCTCGGCGAGCGGGCCCAGGACCTGC
  CCAACCACCCCTGCCGCTTCGACACCAACACCCGCGTCCTGGCGTCCTGCGGCTTCTCCTCGGGCCGGCA
  TCACTGGGAGGTGGAGGTGGGCTCTAAGGACGGCTGGGCCTTTGGCGTGGCCCGCGAGAGCGTGCGCCGA
  AAGGGCCTGACGCCCTTCACTCCCGAGGAGGGCGTCTGGGCCCTGCAGCTCAACGGCGGCCAGTACTGGG
  CCGTGACCAGCCCCGAGCGGTCGCCCCTCAGCTGCGGGCACCTGTCGCGCGTGCGGGTGGCCCTGGACCT
  GGAGGTGGGAGCCGTGTCCTTCTACGCTGTGGAGGACATGCGCCACCTCTACACCTTCCGCGTCAACTTC
  CAGGAGCGCGTGTTCCCGCTTTTCTCTGTTTGCTCCACGGGCACCTACTTGCGAATCTGGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224754 representing NM_203293
  Red=Cloning site Green=Tags(s)
  
  MAAVGPRTGPGTGAEALALAAELQGEATCSICLELFREPVSVECGHSFCRACIGRCWERPGAGSVGAATR
  APPFPLPCPQCREPARPSQLRPNRQLAAVATLLRRFSLPAAAPGEHGSQAAAARAAAARCGQHGEPFKLY
  CQDDGRAICVVCDRAREHREHAVLPLDEAVQEAKELLESRLRVLKKELEDCEVFRSTEKKESKELLKQMA
  AEQEKVGAEFQALRAFLVEQEGRLLGRLEELSREVAQKQNENLAQLGVEITQLSKLSSQIQETAQKPDLD
  FLQEFKSTLSRCSNVPGPKPTTVSSEMKNKVWNVSLKTFVLKGMLKKFKEDLRGELEKEEKVELTLDPDT
  ANPRLILSLDLKGVRLGERAQDLPNHPCRFDTNTRVLASCGFSSGRHHWEVEVGSKDGWAFGVARESVRR
  KGLTPFTPEEGVWALQLNGGQYWAVTSPERSPLSCGHLSRVRVALDLEVGAVSFYAVEDMRHLYTFRVNF
  QERVFPLFSVCSTGTYLRIWP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203293
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203293.3
• RefSeq Size: 2862 bp
• RefSeq ORF: 1536 bp
• Locus ID: 81786
• UniProt ID: Q9C029
• Cytogenetics: 5q35.3
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1, a B-box type 2, and a coiled-coil region. The protein localizes to both the nucleus and the cytoplasm, and may represent a participant in the initiation of glycogen synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]