DBX1 (NM_001029865) Human Tagged ORF Clone

CAT#: RG224662

DBX1 (tGFP-tagged) - Human developing brain homeobox 1 (DBX1)

Product Images

Circular map for RG224662

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DBX1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224662 representing NM_001029865
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTTCCCCGGCCTCCTCGCGCCCCCCGCCGGGTACCCTAGCCTCCTGCGGCCCACGCCCACCTTGA
  CGCTGCCCCAGTCCTTGCAGTCGGCATTTTCCGGCCACTCCAGCTTCCTGGTGGAGGATCTGATCCGCAT
  CAGCCGACCCCCCGCCTACCTGCCCCGCAGCGTGCCCACCGCCAGCATGTCGCCGCCCAGGCAGGGGGCC
  CCCACGGCCCTCACCGACACGGGGGCCTCGGACCTGGGCTCCCCGGGTCCCGGCAGCCGACGGGGCGGCT
  CTCCGCCGACTGCCTTCTCCCCTGCCAGCGAGACGACGTTTCTGAAGTTTGGAGTGAACGCCATCCTCTC
  CTCGGGGCCCAGAACAGAAACATCCCCAGCCTTGCTCCAGAGCGTCCCTCCCAAGACCTTCGCCTTTCCC
  TACTTCGAAGGGTCTTTTCAGCCTTTCATCAGATCTTCTTATTTCCCAGCTTCCTCCAGCGTGGTGCCCA
  TCCCCGGGACCTTCTCCTGGCCGCTGGCCGCGCGCGGGAAGCCTCGGCGGGGCATGCTGCGTCGAGCAGT
  CTTCTCCGACGTGCAGCGGAAGGCGCTGGAGAAGATGTTCCAGAAGCAGAAGTACATCAGCAAGCCCGAC
  CGCAAGAAGCTGGCGGCCAAGCTGGGCCTGAAAGACTCGCAGGACACAGCCCGAAGGACAGAGCCTGCAC
  CCGACTCCACTCCCCGCCCCCGTGCGTCCCCCGGAGCCCCTGATCTGACCTCTGCTTCTCCCTTCTTCTC
  GCCGGCCGGGACATTCCAGGTGAAAATCTGGTTCCAGAACCGACGCATGAAATGGCGGAACTCCAAGGAG
  CGCGAACTCCTGTCTAGCGGGGGCTGTCGCGAGCAGACCCTGCCCACCAAGCTCAATCCGCACCCGGACC
  TCAGCGACGTGGGCCAGAAGGGCCCTGGGAACGAAGAGGAGGAGGAGGGCCCGGGCAGCCCCAGCCACCG
  CCTGGCCTACCACGCGTCCTCCGACCCCCAGCACCTGCGGGACCCGCGGCTGCCAGGGCCGCTGCCCCCC
  TCGCCCGCGCACTCGAGCAGTCCCGGGAAACCTTCGGACTTCTCAGATTCCGAGGAGGAAGAGGAGGGCG
  AGGAACAGGAGGAAATCACCGTGTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224662 representing NM_001029865
  Red=Cloning site Green=Tags(s)
  
  MMFPGLLAPPAGYPSLLRPTPTLTLPQSLQSAFSGHSSFLVEDLIRISRPPAYLPRSVPTASMSPPRQGA
  PTALTDTGASDLGSPGPGSRRGGSPPTAFSPASETTFLKFGVNAILSSGPRTETSPALLQSVPPKTFAFP
  YFEGSFQPFIRSSYFPASSSVVPIPGTFSWPLAARGKPRRGMLRRAVFSDVQRKALEKMFQKQKYISKPD
  RKKLAAKLGLKDSQDTARRTEPAPDSTPRPRASPGAPDLTSASPFFSPAGTFQVKIWFQNRRMKWRNSKE
  RELLSSGGCREQTLPTKLNPHPDLSDVGQKGPGNEEEEEGPGSPSHRLAYHASSDPQHLRDPRLPGPLPP
  SPAHSSSPGKPSDFSDSEEEEEGEEQEEITVS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029865
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029865.1, NP_001025036.1
• RefSeq Size: 1149 bp
• RefSeq ORF: 1032 bp
• Locus ID: 120237
• UniProt ID: A6NMT0
• Cytogenetics: 11p15.1
• Gene Summary: Could have a role in patterning the central nervous system during embryogenesis. Has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. Regulates the transcription factor profile, neurotransmitter phenotype, intraspinal migratory path and axonal trajectory of V0 neurons, features that differentiate them from an adjacent set of V1 neurons (By similarity).[UniProtKB/Swiss-Prot Function]