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CHD2 (NM_001271) Human Tagged ORF Clone
CAT#: RG224580
- TrueORF®
CHD2 (tGFP-tagged) - Human chromodomain helicase DNA binding protein 2 (CHD2), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK
"NM_001271" in other vectors (4)
Product Images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | CHD2 |
| Synonyms | DEE94; EEOC |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224580 representing NM_001271
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGAAATAAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGTCACTCAG CCTCTGAAGAAGCTTCGGGTTCAGACTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAG TGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCA GCAGGTTCCAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAG CTGATGTGAAGAAGATGTGGGAAGAATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACA AGAACCATCGCGATTTAATATTAAGGAAGAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGA GGCCAGAGGCAGCTGAAAAAACAAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAGGCA CCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCTGTCCCCAGAAGAACAGTGCC CAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGCGTGGAAAGAGAAAAAAGCAAGATTCTTCTGATGAG GATGATGATGATGACGAAGCTCCCAAAAGGCAGACTCGTCGAAGAGCGGCTAAAAACGTTAGTTACAAAG AAGATGATGACTTTGAGACTGACTCAGATGATCTCATTGAAATGACTGGAGAAGGAGTTGATGAACAGCA AGATAATAGTGAAACTATTGAAAAGGTCTTAGATTCAAGACTGGGAAAGAAAGGAGCCACTGGAGCATCT ACTACTGTATATGCGATTGAAGCTAATGGCGACCCTAGTGGTGACTTTGACACTGAAAAGGATGAAGGTG AAATCCAGTACCTCATCAAGTGGAAGGGTTGGTCTTACATCCACAGCACATGGGAGAGTGAAGAATCCTT ACAGCAACAGAAAGTGAAGGGCCTAAAAAAACTAGAGAACTTCAAGAAAAAAGAGGACGAAATCAAACAA TGGTTAGGGAAAGTTTCTCCTGAAGATGTAGAATATTTCAATTGCCAACAGGAGCTGGCTTCAGAGTTGA ATAAACAGTATCAGATAGTAGAAAGAGTAATAGCTGTGAAGACAAGTAAATCTACATTGGGTCAAACAGA TTTTCCAGCTCATAGTCGGAAGCCGGCACCCTCAAATGAGCCCGAATATCTATGTAAATGGATGGGACTC CCCTATTCAGAGTGTAGCTGGGAAGATGAAGCCCTCATTGGAAAGAAATTCCAGAATTGCATTGACAGCT TCCACAGTAGGAACAACTCAAAAACCATCCCAACAAGAGAATGCAAGGCCCTGAAGCAGAGACCACGATT TGTAGCTTTAAAGAAACAACCTGCATATTTAGGAGGGGAGAATCTGGAACTTCGAGATTATCAGCTAGAA GGTCTAAACTGGCTAGCTCATTCCTGGTGCAAAAATAATAGTGTAATCCTTGCTGATGAAATGGGCCTAG GAAAGACCATCCAGACCATATCATTCCTCTCCTACCTGTTCCACCAACACCAGCTGTATGGCCCCTTTCT TATAGTCGTCCCTTTATCCACCCTCACCTCATGGCAGAGAGAGTTTGAAATCTGGGCACCAGAGATTAAC GTAGTGGTTTACATAGGTGACCTGATGAGCAGAAATACGATACGGGAATATGAATGGATTCATTCCCAAA CCAAAAGATTGAAGTTCAACGCACTTATAACAACATATGAGATCCTCTTGAAAGATAAGACTGTGCTGGG CAGTATTAACTGGGCCTTTCTGGGAGTGGATGAAGCCCATCGGTTGAAGAATGATGACTCTTTATTGTAT AAAACTCTGATTGATTTCAAGTCCAACCATAGGCTCCTGATTACGGGGACCCCTCTTCAGAATTCCCTCA AAGAGCTCTGGTCCTTGCTGCACTTTATTATGCCGGAGAAGTTTGAATTTTGGGAAGATTTTGAAGAAGA CCATGGGAAGGGGAGAGAAAATGGCTACCAGAGTCTTCATAAGGTGCTAGAGCCTTTCCTTCTCCGGAGA GTCAAAAAAGATGTGGAGAAATCCCTTCCTGCTAAAGTGGAACAGATTCTCAGGGTGGAGATGTCAGCCC TTCAGAAACAGTATTACAAGTGGATTCTGACCAGGAATTACAAGGCTCTTGCCAAAGGAACAAGAGGCAG CACATCTGGTTTTCTTAATATTGTGATGGAACTGAAAAAATGTTGCAACCACTGCTATCTGATTAAACCC CCTGAAGAAAATGAAAGGGAAAATGGACAGGAGATTCTTCTGTCCCTCATAAGGAGCAGTGGGAAGTTGA TTTTATTAGACAAACTGTTGACAAGACTTCGAGAAAGGGGGAATCGAGTGCTTATCTTCTCTCAGATGGT GAGAATGTTGGATATCCTGGCTGAATACCTAACTATTAAACACTATCCTTTCCAGCGTCTGGATGGTTCC ATCAAGGGAGAAATCCGAAAACAGGCACTGGACCACTTCAATGCAGATGGGTCTGAGGACTTCTGTTTCC TGCTCTCGACAAGGGCTGGTGGCCTGGGAATCAATTTGGCTTCAGCGGACACAGTCGTCATCTTTGACTC TGACTGGAACCCCCAGAATGACTTGCAGGCACAAGCCCGAGCGCATAGAATTGGTCAAAAGAAGCAGGTA AATATTTACCGCTTAGTTACAAAGGGGACTGTGGAGGAGGAGATCATAGAACGGGCCAAAAAGAAGATGG TATTAGATCATCTGGTGATTCAGCGCATGGACACCACTGGCCGGACGATCCTGGAAAACAACTCAGGAAG GTCCAACTCAAATCCTTTTAATAAAGAAGAGCTGACAGCTATTTTGAAATTTGGAGCAGAGGATCTCTTC AAAGAACTGGAAGGGGAGGAATCAGAACCTCAGGAAATGGATATAGATGAAATTTTGCGGTTGGCTGAAA CGAGAGAGAATGAAGTGTCAACAAGTGCAACAGATGAACTTCTATCACAGTTTAAGGTTGCCAACTTTGC AACAATGGAAGATGAAGAAGAGCTAGAAGAGCGTCCTCACAAGGACTGGGATGAGATCATTCCAGAGGAA CAAAGGAAAAAAGTAGAGGAGGAAGAGCGGCAGAAGGAGCTAGAAGAAATTTATATGCTGCCTCGAATTC GGAGTTCCACTAAAAAGGCTCAGACAAATGACAGTGACTCTGACACTGAGTCTAAGAGGCAGGCCCAGAG ATCCTCTGCTTCTGAGAGTGAAACGGAAGACTCTGATGATGACAAGAAGCCAAAGCGCAGAGGGCGTCCG AGGAGTGTGCGGAAGGACCTCGTGGAGGGATTTACTGATGCAGAGATCCGAAGGTTCATCAAGGCTTATA AGAAGTTTGGTCTCCCTCTTGAACGGCTGGAGTGCATAGCACGTGATGCTGAGCTGGTAGATAAGTCGGT GGCAGATCTGAAGCGCCTGGGTGAACTGATCCACAACAGCTGTGTGTCAGCAATGCAGGAATACGAAGAG CAGCTGAAAGAAAATGCCAGCGAGGGAAAAGGACCAGGGAAAAGGAGAGGTCCAACAATCAAGATATCCG GAGTTCAGGTTAATGTGAAATCCATTATCCAACATGAAGAGGAGTTTGAGATGCTGCATAAATCTATCCC TGTGGACCCTGAAGAAAAAAAAAAATACTGCTTAACCTGTCGTGTCAAAGCTGCACATTTTGATGTAGAG TGGGGGGTGGAAGATGATTCTCGCCTGTTGCTGGGGATTTATGAACATGGCTATGGAAACTGGGAGTTAA TTAAAACAGACCCAGAGCTTAAATTAACTGACAAAATTCTGCCGGTGGAGACAGATAAAAAGCCTCAGGG GAAGCAGCTACAGACCCGAGCGGATTACTTGTTGAAGCTGCTCAGAAAGGGTCTGGAGAAGAAGGGGGCT GTGACAGGTGGGGAAGAGGCCAAATTAAAGAAGCGGAAGCCTCGGGTAAAGAAGGAAAACAAAGTGCCCA GGCTGAAAGAGGAGCATGGAATTGAGCTTTCATCTCCTAGGCATTCAGATAATCCATCAGAAGAGGGAGA AGTGAAAGATGATGGCTTGGAAAAAAGTCCAATGAAAAAAAAACAGAAGAAGAAAGAGAACAAGGAGAAC AAGGAGAAACAAATGAGTTCTAGGAAAGACAAAGAAGGGGACAAGGAAAGAAAGAAGTCAAAAGATAAGA AAGAGAAGCCTAAAAGTGGTGATGCCAAATCTTCGAGTAAATCAAAGCGATCTCAGGGTCCTGTCCATAT TACAGCAGGAAGTGAACCTGTCCCCATTGGAGAGGATGAGGATGATGATCTGGACCAGGAGACATTCAGC ATATGTAAGGAGAGGATGAGGCCCGTGAAAAAGGCACTGAAACAGCTCGACAAACCTGACAAGGGGCTCA ACGTGCAAGAACAGCTGGAACACACCCGGAACTGCCTGCTGAAAATCGGAGACCGGATAGCCGAGTGCCT TAAAGCCTACTCAGATCAGGAGCACATCAAACTCTGGAGGAGGAACCTATGGATTTTTGTTTCCAAGTTT ACAGAATTTGATGCTCGAAAACTGCATAAGTTATACAAGATGGCTCATAAGAAAAGGTCTCAAGAAGAAG AGGAGCAAAAGAAGAAAGACGACGTGACTGGGGGTAAGAAACCATTTCGTCCAGAGGCCTCAGGCTCCAG CCGGGACTCTCTGATATCTCAGTCCCATACCTCACACAACCTTCACCCTCAGAAGCCTCATTTGCCTGCC TCCCATGGCCCACAGATGCATGGACACCCAAGAGATAACTACAATCACCCCAACAAGAGACACTTCAGTA ATGCAGATCGAGGAGACTGGCAGAGGGAAAGAAAGTTCAACTATGGTGGTGGCAACAACAATCCACCATG GGGAAGCGACAGGCACCATCAGTATGAGCAGCACTGGTACAAGGACCACCATTATGGGGACCGGCGACAT ATGGATGCCCACCGTTCCGGAAGCTATCGACCCAACAACATGTCCAGAAAGAGGCCTTATGACCAGTACA GCAGTGACCGAGACCACCGGGGACACAGAGATTATTATGACAGGCACCATCATGACTCCAAGCGGAGGAG ATCCGATGAATTTAGGCCTCAAAATTACCACCAGCAGGATTTCCGACGAATGTCTGATCACCGCCCCGCT ATGGGCTACCATGGCCAGGGACCCTCAGACCATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATA AACAGCCTCTACCCCCATTGCACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCA CGATTCCAAGTCACCCCTGGATCATAGGTCTCCTTTGGAGAGATCACTAGAACAGAAAAACAACCCAGAT TATAACTGGAATGTTCGGAAAACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224580 representing NM_001271
Red=Cloning site Green=Tags(s) MMRNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSDPGSGHGSESNSSSESSESQSESESES AGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDVYGVRRSNRSRQEPSRFNIKEEASSGSESGSPKRR GQRQLKKQEKWKQEPSEDEQEQGTSAESEPEQKKVKARRPVPRRTVPKPRVKKQPKTQRGKRKKQDSSDE DDDDDEAPKRQTRRRAAKNVSYKEDDDFETDSDDLIEMTGEGVDEQQDNSETIEKVLDSRLGKKGATGAS TTVYAIEANGDPSGDFDTEKDEGEIQYLIKWKGWSYIHSTWESEESLQQQKVKGLKKLENFKKKEDEIKQ WLGKVSPEDVEYFNCQQELASELNKQYQIVERVIAVKTSKSTLGQTDFPAHSRKPAPSNEPEYLCKWMGL PYSECSWEDEALIGKKFQNCIDSFHSRNNSKTIPTRECKALKQRPRFVALKKQPAYLGGENLELRDYQLE GLNWLAHSWCKNNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFLIVVPLSTLTSWQREFEIWAPEIN VVVYIGDLMSRNTIREYEWIHSQTKRLKFNALITTYEILLKDKTVLGSINWAFLGVDEAHRLKNDDSLLY KTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFEFWEDFEEDHGKGRENGYQSLHKVLEPFLLRR VKKDVEKSLPAKVEQILRVEMSALQKQYYKWILTRNYKALAKGTRGSTSGFLNIVMELKKCCNHCYLIKP PEENERENGQEILLSLIRSSGKLILLDKLLTRLRERGNRVLIFSQMVRMLDILAEYLTIKHYPFQRLDGS IKGEIRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQV NIYRLVTKGTVEEEIIERAKKKMVLDHLVIQRMDTTGRTILENNSGRSNSNPFNKEELTAILKFGAEDLF KELEGEESEPQEMDIDEILRLAETRENEVSTSATDELLSQFKVANFATMEDEEELEERPHKDWDEIIPEE QRKKVEEEERQKELEEIYMLPRIRSSTKKAQTNDSDSDTESKRQAQRSSASESETEDSDDDKKPKRRGRP RSVRKDLVEGFTDAEIRRFIKAYKKFGLPLERLECIARDAELVDKSVADLKRLGELIHNSCVSAMQEYEE QLKENASEGKGPGKRRGPTIKISGVQVNVKSIIQHEEEFEMLHKSIPVDPEEKKKYCLTCRVKAAHFDVE WGVEDDSRLLLGIYEHGYGNWELIKTDPELKLTDKILPVETDKKPQGKQLQTRADYLLKLLRKGLEKKGA VTGGEEAKLKKRKPRVKKENKVPRLKEEHGIELSSPRHSDNPSEEGEVKDDGLEKSPMKKKQKKKENKEN KEKQMSSRKDKEGDKERKKSKDKKEKPKSGDAKSSSKSKRSQGPVHITAGSEPVPIGEDEDDDLDQETFS ICKERMRPVKKALKQLDKPDKGLNVQEQLEHTRNCLLKIGDRIAECLKAYSDQEHIKLWRRNLWIFVSKF TEFDARKLHKLYKMAHKKRSQEEEEQKKKDDVTGGKKPFRPEASGSSRDSLISQSHTSHNLHPQKPHLPA SHGPQMHGHPRDNYNHPNKRHFSNADRGDWQRERKFNYGGGNNNPPWGSDRHHQYEQHWYKDHHYGDRRH MDAHRSGSYRPNNMSRKRPYDQYSSDRDHRGHRDYYDRHHHDSKRRRSDEFRPQNYHQQDFRRMSDHRPA MGYHGQGPSDHYRSFHTDKLGEYKQPLPPLHPAVSDPRSPPSQKSPHDSKSPLDHRSPLERSLEQKNNPD YNWNVRKT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001271 |
| ORF Size | 5484 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001271.4 |
| RefSeq Size | 9374 bp |
| RefSeq ORF | 5487 bp |
| Locus ID | 1106 |
| UniProt ID | O14647 |
| Cytogenetics | 15q26.1 |
| Domains | SNF2_N, CHROMO, DEAD, helicase_C |
| Protein Families | Transcription Factors |
| Gene Summary | The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224580 | CHD2 (Myc-DDK-tagged)-Human chromodomain helicase DNA binding protein 2 (CHD2), transcript variant 1 |
USD 2,304.00 |
|
| RC224580L1 | Lenti ORF clone of Human chromodomain helicase DNA binding protein 2 (CHD2), transcript variant 1, Myc-DDK-tagged |
USD 2,604.00 |
|
| SC119350 | CHD2 (untagged)-Human chromodomain helicase DNA binding protein 2 (CHD2), transcript variant 1 |
USD 1,462.00 |
|
| SC318677 | CHD2 (untagged)-Human chromodomain helicase DNA binding protein 2 (CHD2), transcript variant 1 |
USD 1,690.00 |
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