DPP9 (NM_139159) Human Tagged ORF Clone

CAT#: RG224465

DPP9 (tGFP-tagged) - Human dipeptidyl-peptidase 9 (DPP9)

Product Images

Circular map for RG224465

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DPP9
• Synonyms: DP9; DPLP9; DPP IX; DPRP-2; DPRP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224465 representing NM_139159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCACCGGGACCCCAACGGCCGACCGAGGCGACGCAGCCGCCACAGATGACCCGGCCGCCCGCT
  TCCAGGTGCAGAAGCACTCGTGGGACGGGCTCCGGAGCATCATCCACGGCAGCCGCAAGTACTCGGGCCT
  CATTGTCAACAAGGCGCCCCACGACTTCCAGTTTGTGCAGAAGACGGATGAGTCTGGGCCCCACTCCCAC
  CGCCTCTACTACCTGGGAATGCCATATGGCAGCCGAGAGAACTCCCTCCTCTACTCTGAGATTCCCAAGA
  AGGTCCGGAAAGAGGCTCTGCTGCTCCTGTCCTGGAAGCAGATGCTGGATCATTTCCAGGCCACGCCCCA
  CCATGGGGTCTACTCTCGGGAGGAGGAGCTGCTGAGGGAGCGGAAACGCCTGGGGGTCTTCGGCATCACC
  TCCTACGACTTCCACAGCGAGAGTGGCCTCTTCCTCTTCCAGGCCAGCAACAGCCTCTTCCACTGCCGCG
  ACGGCGGCAAGAACGGCTTCATGGTGTCCCCTATGAAACCGCTGGAAATCAAGACCCAGTGCTCAGGGCC
  CCGGATGGACCCCAAAATCTGCCCTGCCGACCCTGCCTTCTTCTCCTTCATCAATAACAGCGACCTGTGG
  GTGGCCAACATCGAGACAGGCGAGGAGCGGCGGCTGACCTTCTGCCACCAAGGTTTATCCAATGTCCTGG
  ATGACCCCAAGTCTGCGGGTGTGGCCACCTTCGTCATACAGGAAGAGTTCGACCGCTTCACTGGGTACTG
  GTGGTGCCCCACAGCCTCCTGGGAAGGTTCAGAGGGCCTCAAGACGCTGCGAATCCTGTATGAGGAAGTC
  GATGAGTCCGAGGTGGAGGTCATTCACGTCCCCTCTCCTGCGCTAGAAGAAAGGAAGACGGACTCGTATC
  GGTACCCCAGGACAGGCAGCAAGAATCCCAAGATTGCCTTGAAACTGGCTGAGTTCCAGACTGACAGCCA
  GGGCAAGATCGTCTCGACCCAGGAGAAGGAGCTGGTGCAGCCCTTCAGCTCGCTGTTCCCGAAGGTGGAG
  TACATCGCCAGGGCCGGGTGGACCCGGGATGGCAAATACGCCTGGGCCATGTTCCTGGACCGGCCCCAGC
  AGTGGCTCCAGCTCGTCCTCCTCCCCCCGGCCCTGTTCATCCCGAGCACAGAGAATGAGGAGCAGCGGCT
  AGCCTCTGCCAGAGCTGTCCCCAGGAATGTCCAGCCGTATGTGGTGTACGAGGAGGTCACCAACGTCTGG
  ATCAATGTTCATGACATCTTCTATCCCTTCCCCCAATCAGAGGGAGAGGACGAGCTCTGCTTTCTCCGCG
  CCAATGAATGCAAGACCGGCTTCTGCCATTTGTACAAAGTCACCGCCGTTTTAAAATCCCAGGGCTACGA
  TTGGAGTGAGCCCTTCAGCCCCGGGGAAGATGAATTTAAGTGCCCCATTAAGGAAGAGATTGCTCTGACC
  AGCGGTGAATGGGAGGTTTTGGCGAGGCACGGCTCCAAGGGCACCAAGGACACGCCGCTGGAGCACCACC
  TCTACGTGGTCAGCTATGAGGCGGCCGGCGAGATCGTACGCCTCACCACGCCCGGCTTCTCCCATAGCTG
  CTCCATGAGCCAGAACTTCGACATGTTCGTCAGCCACTACAGCAGCGTGAGCACGCCGCCCTGCGTGCAC
  GTCTACAAGCTGAGCGGCCCCGACGACGACCCCCTGCACAAGCAGCCCCGCTTCTGGGCTAGCATGATGG
  AGGCAGCCAGCTGCCCCCCGGATTATGTTCCTCCAGAGATCTTCCATTTCCACACGCGCTCGGATGTGCG
  GCTCTACGGCATGATCTACAAGCCCCACGCCTTGCAGCCAGGGAAGAAGCACCCCACCGTCCTCTTTGTA
  TATGGAGGCCCCCAGGTGCAGCTGGTGAATAACTCCTTCAAAGGCATCAAGTACTTGCGGCTCAACACAC
  TGGCCTCCCTGGGCTACGCCGTGGTTGTGATTGACGGCAGGGGCTCCTGTCAGCGAGGGCTTCGGTTCGA
  AGGGGCCCTGAAAAACCAAATGGGCCAGGTGGAGATCGAGGACCAGGTGGAGGGCCTGCAGTTCGTGGCC
  GAGAAGTATGGCTTCATCGACCTGAGCCGAGTTGCCATCCATGGCTGGTCCTACGGGGGCTTCCTCTCGC
  TCATGGGGCTAATCCACAAGCCCCAGGTGTTCAAGGTGGCCATCGCGGGTGCCCCGGTCACCGTCTGGAT
  GGCCTACGACACAGGGTACACTGAGCGCTACATGGACGTCCCTGAGAACAACCAGCACGGCTATGAGGCG
  GGTTCCGTGGCCCTGCACGTGGAGAAGCTGCCCAATGAGCCCAACCGCTTGCTTATCCTCCACGGCTTCC
  TGGACGAAAACGTGCACTTTTTCCACACAAACTTCCTCGTCTCCCAACTGATCCGAGCAGGGAAACCTTA
  CCAGCTCCAGATCTACCCCAACGAGAGACACAGTATTCGCTGCCCCGAGTCGGGCGAGCACTATGAAGTC
  ACGTTGCTGCACTTTCTACAGGAATACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224465 representing NM_139159
  Red=Cloning site Green=Tags(s)
  
  MATTGTPTADRGDAAATDDPAARFQVQKHSWDGLRSIIHGSRKYSGLIVNKAPHDFQFVQKTDESGPHSH
  RLYYLGMPYGSRENSLLYSEIPKKVRKEALLLLSWKQMLDHFQATPHHGVYSREEELLRERKRLGVFGIT
  SYDFHSESGLFLFQASNSLFHCRDGGKNGFMVSPMKPLEIKTQCSGPRMDPKICPADPAFFSFINNSDLW
  VANIETGEERRLTFCHQGLSNVLDDPKSAGVATFVIQEEFDRFTGYWWCPTASWEGSEGLKTLRILYEEV
  DESEVEVIHVPSPALEERKTDSYRYPRTGSKNPKIALKLAEFQTDSQGKIVSTQEKELVQPFSSLFPKVE
  YIARAGWTRDGKYAWAMFLDRPQQWLQLVLLPPALFIPSTENEEQRLASARAVPRNVQPYVVYEEVTNVW
  INVHDIFYPFPQSEGEDELCFLRANECKTGFCHLYKVTAVLKSQGYDWSEPFSPGEDEFKCPIKEEIALT
  SGEWEVLARHGSKGTKDTPLEHHLYVVSYEAAGEIVRLTTPGFSHSCSMSQNFDMFVSHYSSVSTPPCVH
  VYKLSGPDDDPLHKQPRFWASMMEAASCPPDYVPPEIFHFHTRSDVRLYGMIYKPHALQPGKKHPTVLFV
  YGGPQVQLVNNSFKGIKYLRLNTLASLGYAVVVIDGRGSCQRGLRFEGALKNQMGQVEIEDQVEGLQFVA
  EKYGFIDLSRVAIHGWSYGGFLSLMGLIHKPQVFKVAIAGAPVTVWMAYDTGYTERYMDVPENNQHGYEA
  GSVALHVEKLPNEPNRLLILHGFLDENVHFFHTNFLVSQLIRAGKPYQLQIYPNERHSIRCPESGEHYEV
  TLLHFLQEYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139159
• ORF Size: 2550 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139159.3, NP_631898.2
• RefSeq Size: 4274 bp
• RefSeq ORF: 2679 bp
• Locus ID: 91039
• UniProt ID: Q86TI2
• Cytogenetics: 19p13.3
• Domains: Peptidase_S9, DPPIV_N_term
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq, Jul 2008]