TPTE (NM_199261) Human Tagged ORF Clone

CAT#: RG223844

TPTE (tGFP-tagged) - Human transmembrane phosphatase with tensin homology (TPTE), transcript variant 1

Product Images

Circular map for RG223844

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TPTE
• Synonyms: CT44; PTEN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223844 representing NM_199261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAAAGTCCTGATCCGACTGACCTGGCGGGAGTCATCATTGAGCTCGGCCCCAATGACAGTCCAC
  AGACAAGTGAATTTAAAGGAGCAACCGAGGAGGCACCTGCGAAAGAAAGCCCACACACAAGTGAATTTAA
  AGGAGCAGCCCGGGTGTCACCTATCAGTGAAAGTGTGTTAGCACGACTTTCCAAGTTTGAAGTTGAAGAT
  GCTGAAAATGTTGCTTCATATGACAGCAAGATTAAGAAAATTGTGCATTCAATTGTATCATCCTTTGCAT
  TTGGACTATTTGGAGTTTTCCTGGTCTTACTGGATGTCACTCTCATCCTTGCCGACCTAATTTTCACTGA
  CAGCAAACTTTATATTCCTTTGGAGTATCGTTCTATTTCTCTAGCTATTGCCTTATTTTTTCTCATGGAT
  GTTCTTCTTCGAGTATTTGTAGAAAGGAGACAGCAGTATTTTTCTGACTTATTTAACATTTTAGATACTG
  CCATTATTGTGATTCTTCTGCTGGTTGATGTCGTTTACATTTTTTTTGACATTAAGTTGCTTAGGAATAT
  TCCCAGATGGACACATTTACTTCGACTTCTACGACTTATTATTCTGTTAAGAATTTTTCATCTGTTTCAT
  CAAAAAAGACAACTTGAAAAGCTGATAAGAAGGCGGGTTTCAGAAAACAAAAGGCGATACACAAGGGATG
  GATTTGACCTAGACCTCACTTACGTTACAGAACGTATTATTGCTATGTCATTTCCATCTTCTGGAAGGCA
  GTCTTTCTATAGAAATCCAATCAAGGAAGTTGTGCGGTTTCTAGATAAGAAACACCGAAACCACTATCGA
  GTCTACAATCTATGCAGTGAAAGAGCTTACGATCCTAAGCACTTCCATAATAGGGTCGTTAGAATCATGA
  TTGATGATCATAATGTCCCCACTCTACATCAGATGGTGGTTTTCACCAAGGAAGTAAATGAGTGGATGGC
  TCAAGATCTTGAAAACATCGTAGCGATTCACTGTAAAGGAGGCACAGATAGAACAGGAACTATGGTTTGT
  GCCTTCCTTATTGCCTCTGAAATATGTTCAACTGCAAAGGAAAGCCTGTATTATTTTGGAGAAAGGCGAA
  CAGATAAAACCCACAGCGAAAAATTTCAGGGAGTAGAAACTCCTTCTCAGAAGAGATATGTTGCATATTT
  TGCACAAGTGAAACATCTCTACAACTGGAATCTCCCTCCAAGACGGATACTCTTTATAAAACACTTCATT
  ATTTATTCGATTCCTCGTTATGTACGTGATCTAAAAATCCAAATAGAAATGGAGAAAAAGGTTGTCTTTT
  CCACTATTTCATTAGGAAAATGTTCGGTACTTGATAACATTACAACAGACAAAATATTAATTGATGTATT
  CGACGGTCTACCTCTGTATGATGATGTGAAAGTGCAGTTTTTCTATTCGAATCTTCCTACATACTATGAC
  AATTGCTCATTTTACTTCTGGTTGCACACATCTTTTATTGAAAATAACAGGCTTTATCTACCAAAAAATG
  AATTGGATAATCTACATAAACAAAAAGCACGGAGAATTTATCCATCAGATTTTGCCGTGGAGATACTTTT
  TGGCGAGAAAATGACTTCCAGTGATGTTGTAGCTGGATCCGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223844 representing NM_199261
  Red=Cloning site Green=Tags(s)
  
  MNESPDPTDLAGVIIELGPNDSPQTSEFKGATEEAPAKESPHTSEFKGAARVSPISESVLARLSKFEVED
  AENVASYDSKIKKIVHSIVSSFAFGLFGVFLVLLDVTLILADLIFTDSKLYIPLEYRSISLAIALFFLMD
  VLLRVFVERRQQYFSDLFNILDTAIIVILLLVDVVYIFFDIKLLRNIPRWTHLLRLLRLIILLRIFHLFH
  QKRQLEKLIRRRVSENKRRYTRDGFDLDLTYVTERIIAMSFPSSGRQSFYRNPIKEVVRFLDKKHRNHYR
  VYNLCSERAYDPKHFHNRVVRIMIDDHNVPTLHQMVVFTKEVNEWMAQDLENIVAIHCKGGTDRTGTMVC
  AFLIASEICSTAKESLYYFGERRTDKTHSEKFQGVETPSQKRYVAYFAQVKHLYNWNLPPRRILFIKHFI
  IYSIPRYVRDLKIQIEMEKKVVFSTISLGKCSVLDNITTDKILIDVFDGLPLYDDVKVQFFYSNLPTYYD
  NCSFYFWLHTSFIENNRLYLPKNELDNLHKQKARRIYPSDFAVEILFGEKMTSSDVVAGSD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199261
• ORF Size: 1653 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199261.3, NP_954870.2
• RefSeq Size: 2199 bp
• RefSeq ORF: 1656 bp
• Locus ID: 7179
• UniProt ID: P56180
• Cytogenetics: 21p11.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a PTEN-related tyrosine phosphatase which may play a role in the signal transduction pathways of the endocrine or spermatogenic function of the testis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]