C19orf51 (DNAAF3) (NM_178837) Human Tagged ORF Clone

CAT#: RG223043

DNAAF3 (tGFP-tagged) - Human chromosome 19 open reading frame 51 (C19orf51)

Product Images

Circular map for RG223043

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C19orf51
• Synonyms: C19orf51; CILD2; DAB1; PCD; PF22
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223043 representing NM_178837
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCCATTGCTTGATTCCTCGAAAAGGGCGGGGACTCTGGGCAGCGGTTGTGGAGTGCCGCGGGTCC
  ACAGTGCTGCACTGAGCAGAGAGGAAGGGGCGTCCCGGGATATTTGGAGGATAAAGGTGTGGGCAAGGGT
  GATGACCACACCTGCCGGCTCCGGCAGCGGCTTCGGCTCCGTGTCCTGGTGGGGCCTGTCCCCGGCGCTG
  GACCTGCAGGCTGAAAGTCCTCCTGTGGACCCAGACTCCCAGGCCGATACAGTGCACAGCAACCCCGAGC
  TAGATGTGCTGCTTCTGGGCTCTGTGGATGGACGGCACCTGCTGCGGACCCTGTCCCGAGCGAAGTTCTG
  GCCTCGCAGGAGGTTCAACTTCTTTGTGCTGGAGAATAATCTGGAAGCTGTGGCCCGACACATGCTGATC
  TTCAGCCTAGCCCTGGAGGAACCGGAGAAGATGGGGCTGCAAGAGCGAAGCGAGACCTTCCTGGAAGTGT
  GGGGGAACGCGCTGCTGCGCCCGCCAGTGGCCGCCTTCGTGCGTGCCCAGGCCGACCTGCTGGCGCACCT
  GGTCCCCGAGCCCGACCGCCTGGAGGAACAGCTGCCCTGGCTCAGCCTCCGCGCCCTCAAGTTCCGCGAG
  CGGGATGCCCTGGAGGCCGTATTCCGCTTCTGGGCTGGCGGCGAGAAAGGGCCCCAGGCGTTCCCCATGA
  GCCGCCTCTGGGACTCGCGCCTGCGCCACTACCTGGGCTCCCGCTACGACGCCCGGCGCGGTGTCAGCGA
  CTGGGACCTGCGCATGAAGCTGCATGACCGCGGGGCTCAAGTCATTCACCCCCAGGAGTTCCGACGCTGG
  CGGGACACAGGCGTCGCCTTTGAACTCAGGGACTCCAGCGCCTATCATGTGCCCAACCGGACCCTGGCGT
  CCGGTCGCCTCCTGAGCTACCGTGGGGAGCGCGTGGCAGCGCGCGGGTACTGGGGGGACATCGCCACGGG
  GCCCTTCGTGGCCTTCGGCATCGAAGCGGACGACGAGAGCCTCCTGCGGACGAGCAACGGCCAGCCAGTC
  AAGACGGCCGGGGAGATCACTCAACACAACGTGACGGAGCTGCTCCGCGACGTGGCCGCCTGGGGGCGCG
  CGAGAGCCACCGGGGGGGACCTGGAGGAGCAGCAGCACGCGGAGGGAAGCCCGGAGCCAGGGACTCCAGC
  AGCCCCGACCCCGGAATCTTTCACCGTCCACTTCCTGCCGCTCAATTCTGCTCAGACTCTCCACCACAAG
  AGCTGCTACAACGGCCGATTCCAGCTCCTCTATGTGGCCTGTGGTATGGTCCATCTTCTCATCCCTGAGC
  TTGGGGCCTGTGTGGCACCCGGAGGGAACTTGATTGTGGAATTAGCCCGGTACCTGGTGGACGTGCGGCA
  GGAGCAGCTGCAGGGATTCAACACCCGGGTCAGGGAGCTAGCTCAGGCAGCTGGATTTGCTCCACAGACC
  GGGGCCAGGCCTTCAGAGACCTTCGCACGTTTCTGCAAGTCCCAGGAATCAGCTCTGGGCAACACTGTCC
  CAGCTGTGGAACCCGGAACTCCGCCCCTTGACATCCTGGCCCAGCCTCTTGAAGCCAGCAACCCAGCCCT
  TGAGGGCCTGACCCAGCCTCTGCAGGGTGGGACCCCACACTGTGAGCCCTGCCAGCTGCCCTCTGAGTCT
  CCAGGTTCACTCTCAGAGGTTCTGGCTCAGCCTCAGGGGGCCTTGGCTCCGCCCAACTGTGAGTCAGACT
  CCAAAACTGGAGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223043 representing NM_178837
  Red=Cloning site Green=Tags(s)
  
  MLPLLDSSKRAGTLGSGCGVPRVHSAALSREEGASRDIWRIKVWARVMTTPAGSGSGFGSVSWWGLSPAL
  DLQAESPPVDPDSQADTVHSNPELDVLLLGSVDGRHLLRTLSRAKFWPRRRFNFFVLENNLEAVARHMLI
  FSLALEEPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQADLLAHLVPEPDRLEEQLPWLSLRALKFRE
  RDALEAVFRFWAGGEKGPQAFPMSRLWDSRLRHYLGSRYDARRGVSDWDLRMKLHDRGAQVIHPQEFRRW
  RDTGVAFELRDSSAYHVPNRTLASGRLLSYRGERVAARGYWGDIATGPFVAFGIEADDESLLRTSNGQPV
  KTAGEITQHNVTELLRDVAAWGRARATGGDLEEQQHAEGSPEPGTPAAPTPESFTVHFLPLNSAQTLHHK
  SCYNGRFQLLYVACGMVHLLIPELGACVAPGGNLIVELARYLVDVRQEQLQGFNTRVRELAQAAGFAPQT
  GARPSETFARFCKSQESALGNTVPAVEPGTPPLDILAQPLEASNPALEGLTQPLQGGTPHCEPCQLPSES
  PGSLSEVLAQPQGALAPPNCESDSKTGV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178837
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178837.4, NP_849159.2
• RefSeq Size: 2210 bp
• RefSeq ORF: 1767 bp
• Locus ID: 352909
• UniProt ID: Q8N9W5
• Cytogenetics: 19q13.42
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is required for the assembly of axonemal inner and outer dynein arms and plays a role in assembling dynein complexes for transport into cilia. Defects in this gene are a cause of primary ciliary dyskinesia type 2 (CILD2). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]