NOS1 (NM_000620) Human Tagged ORF Clone

CAT#: RG222384

NOS1 (tGFP-tagged) - Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1

Product Images

Circular map for RG222384

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NOS1
• Synonyms: bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222384 representing NM_000620
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATCACATGTTCGGTGTTCAGCAAATCCAGCCCAATGTCATTTCTGTTCGTCTCTTCAAGCGCA
  AAGTTGGGGGCCTGGGATTTCTGGTGAAGGAGCGGGTCAGTAAGCCGCCCGTGATCATCTCTGACCTGAT
  TCGTGGGGGCGCCGCAGAGCAGAGTGGCCTCATCCAGGCCGGAGACATCATTCTTGCGGTCAACGGCCGG
  CCCTTGGTGGACCTGAGCTATGACAGCGCCCTGGAGGTACTCAGAGGCATTGCCTCTGAGACCCACGTGG
  TCCTCATTCTGAGGGGCCCTGAAGGTTTCACCACGCACCTGGAGACCACCTTTACAGGTGATGGGACCCC
  CAAGACCATCCGGGTGACACAGCCCCTGGGTCCCCCCACCAAAGCCGTGGATCTGTCCCACCAGCCACCG
  GCCGGCAAAGAACAGCCCCTGGCAGTGGATGGGGCCTCGGGTCCCGGGAATGGGCCTCAGCATGCCTACG
  ATGATGGGCAGGAGGCTGGCTCACTCCCCCATGCCAACGGCCTGGCCCCCAGGCCCCCAGGCCAGGACCC
  CGCGAAGAAAGCAACCAGAGTCAGCCTCCAAGGCAGAGGGGAGAACAATGAACTGCTCAAGGAGATAGAG
  CCTGTGCTGAGCCTTCTCACCAGTGGGAGCAGAGGGGTCAAGGGAGGGGCACCTGCCAAGGCAGAGATGA
  AAGATATGGGAATCCAGGTGGACAGAGATTTGGACGGCAAGTCACACAAACCTCTGCCCCTCGGCGTGGA
  GAACGACCGAGTCTTCAATGACCTATGGGGGAAGGGCAATGTGCCTGTCGTCCTCAACAACCCATATTCA
  GAGAAGGAGCAGCCCCCCACCTCAGGAAAACAGTCCCCCACAAAGAATGGCAGCCCCTCCAAGTGTCCAC
  GCTTCCTCAAGGTCAAGAACTGGGAGACTGAGGTGGTTCTCACTGACACCCTCCACCTTAAGAGCACATT
  GGAAACGGGATGCACTGAGTACATCTGCATGGGCTCCATCATGCATCCTTCTCAGCATGCAAGGAGGCCT
  GAAGACGTCCGCACAAAAGGACAGCTCTTCCCTCTCGCCAAAGAGTTTATTGATCAATACTATTCATCAA
  TTAAAAGATTTGGCTCCAAAGCCCACATGGAAAGGCTGGAAGAGGTGAACAAAGAGATCGACACCACTAG
  CACTTACCAGCTCAAGGACACAGAGCTCATCTATGGGGCCAAGCACGCCTGGCGGAATGCCTCGCGCTGT
  GTGGGCAGGATCCAGTGGTCCAAGCTGCAGGTATTCGATGCCCGTGACTGCACCACGGCCCACGGGATGT
  TCAACTACATCTGTAACCATGTCAAGTATGCCACCAACAAAGGGAACCTCAGGTCTGCCATCACCATATT
  CCCCCAGAGGACAGACGGCAAGCACGACTTCCGAGTCTGGAACTCCCAGCTCATCCGCTACGCTGGCTAC
  AAGCAGCCTGACGGCTCCACCCTGGGGGACCCAGCCAATGTGCAGTTCACAGAGATATGCATACAGCAGG
  GCTGGAAACCGCCTAGAGGCCGCTTCGATGTCCTGCCGCTCCTGCTTCAGGCCAACGGCAATGACCCTGA
  GCTCTTCCAGATTCCTCCAGAGCTGGTGTTGGAAGTTCCCATCAGGCACCCCAAGTTTGAGTGGTTCAAG
  GACCTGGGGCTGAAGTGGTACGGCCTCCCCGCCGTGTCCAACATGCTCCTAGAGATTGGCGGCCTGGAGT
  TCAGCGCCTGTCCCTTCAGTGGCTGGTACATGGGCACAGAGATTGGTGTCCGCGACTACTGTGACAACTC
  CCGCTACAATATCCTGGAGGAAGTGGCCAAGAAGATGAACTTAGACATGAGGAAGACGTCCTCCCTGTGG
  AAGGACCAGGCGCTGGTGGAGATCAATATCGCGGTTCTCTATAGCTTCCAGAGTGACAAAGTGACCATTG
  TTGACCATCACTCCGCCACCGAGTCCTTCATTAAGCACATGGAGAATGAGTACCGCTGCCGGGGGGGCTG
  CCCTGCCGACTGGGTGTGGATCGTGCCCCCCATGTCCGGAAGCATCACCCCTGTGTTCCACCAGGAGATG
  CTCAACTACCGGCTCACCCCCTCCTTCGAATACCAGCCTGATCCCTGGAACACGCATGTCTGGAAAGGCA
  CCAACGGGACCCCCACAAAGCGGCGAGCCATCGGCTTCAAGAAGCTAGCAGAAGCTGTCAAGTTCTCGGC
  CAAGCTGATGGGGCAGGCTATGGCCAAGAGGGTGAAAGCGACCATCCTCTATGCCACAGAGACAGGCAAA
  TCGCAAGCTTATGCCAAGACCTTGTGTGAGATCTTCAAACACGCCTTTGATGCCAAGGTGATGTCCATGG
  AAGAATATGACATTGTGCACCTGGAACATGAAACTCTGGTCCTTGTGGTCACCAGCACCTTTGGCAATGG
  AGATCCCCCTGAGAATGGGGAGAAATTCGGCTGTGCTTTGATGGAAATGAGGCACCCCAACTCTGTGCAG
  GAAGAAAGGAAGAGCTACAAGGTCCGATTCAACAGCGTCTCCTCCTACTCTGACTCCCAAAAATCATCAG
  GCGATGGGCCCGACCTCAGAGACAACTTTGAGAGTGCTGGACCCCTGGCCAATGTGAGGTTCTCAGTTTT
  TGGCCTCGGCTCACGAGCATACCCTCACTTTTGCGCCTTCGGACACGCTGTGGACACCCTCCTGGAAGAA
  CTGGGAGGGGAGAGGATCTTGAAGATGAGGGAAGGGGATGAGCTCTGTGGGCAGGAAGAGGCTTTCAGGA
  CCTGGGCCAAGAAGGTCTTCAAGGCAGCCTGTGATGTCTTCTGTGTGGGAGATGATGTCAACATTGAAAA
  GGCCAACAATTCCCTCATCAGCAATGATCGCAGCTGGAAGAGAAACAAGTTCCGCCTCACCTTTGTGGCC
  GAAGCTCCAGAACTCACACAAGGTCTATCCAATGTCCACAAAAAGCGAGTCTCAGCTGCCCGGCTCCTTA
  GCCGTCAAAACCTCCAGAGCCCTAAATCCAGTCGGTCAACTATCTTCGTGCGTCTCCACACCAACGGGAG
  CCAGGAGCTGCAGTACCAGCCTGGGGACCACCTGGGTGTCTTCCCTGGCAACCACGAGGACCTCGTGAAT
  GCCCTGATCGAGCGGCTGGAGGACGCGCCGCCTGTCAACCAGATGGTGAAAGTGGAACTGCTGGAGGAGC
  GGAACACGGCTTTAGGTGTCATCAGTAACTGGACAGACGAGCTCCGCCTCCCGCCCTGCACCATCTTCCA
  GGCCTTCAAGTACTACCTGGACATCACCACGCCACCAACGCCTCTGCAGCTGCAGCAGTTTGCCTCCCTA
  GCTACCAGCGAGAAGGAGAAGCAGCGTCTGCTGGTCCTCAGCAAGGGTTTGCAGGAGTACGAGGAATGGA
  AATGGGGCAAGAACCCCACCATCGTGGAGGTGCTGGAGGAGTTCCCATCTATCCAGATGCCGGCCACCCT
  GCTCCTGACCCAGCTGTCCCTGCTGCAGCCCCGCTACTATTCCATCAGCTCCTCCCCAGACATGTACCCT
  GATGAAGTGCACCTCACTGTGGCCATCGTTTCCTACCGCACTCGAGATGGAGAAGGACCAATTCACCACG
  GCGTATGCTCCTCCTGGCTCAACCGGATACAGGCTGACGAACTGGTCCCCTGTTTCGTGAGAGGAGCACC
  CAGCTTCCACCTGCCCCGGAACCCCCAAGTCCCCTGCATCCTCGTTGGACCAGGCACCGGCATTGCCCCT
  TTCCGAAGCTTCTGGCAACAGCGGCAATTTGATATCCAACACAAAGGAATGAACCCCTGCCCCATGGTCC
  TGGTCTTCGGGTGCCGGCAATCCAAGATAGATCATATCTACAGGGAAGAGACCCTGCAGGCCAAGAACAA
  GGGGGTCTTCAGAGAGCTGTACACGGCTTACTCCCGGGAGCCAGACAAACCAAAGAAGTACGTGCAGGAC
  ATCCTGCAGGAGCAGCTGGCGGAGTCTGTGTACCGAGCCCTGAAGGAGCAAGGGGGCCACATATACGTCT
  GTGGGGACGTCACCATGGCTGCTGATGTCCTCAAAGCCATCCAGCGCATCATGACCCAGCAGGGGAAGCT
  CTCGGCAGAGGACGCCGGCGTATTCATCAGCCGGATGAGGGATGACAACCGATACCATGAGGATATTTTT
  GGAGTCACCCTGCGAACGTACGAAGTGACCAACCGCCTTAGATCTGAGTCCATTGCCTTCATTGAAGAGA
  GCAAAAAAGACACCGATGAGGTTTTCAGCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222384 representing NM_000620
  Red=Cloning site Green=Tags(s)
  
  MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGR
  PLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPP
  AGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIE
  PVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYS
  EKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRP
  EDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRC
  VGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGY
  KQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFK
  DLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLW
  KDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEM
  LNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGK
  SQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQ
  EERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEE
  LGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVA
  EAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVN
  ALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASL
  ATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYP
  DEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAP
  FRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQD
  ILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIF
  GVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000620
• ORF Size: 4302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000620.1, NP_000611.1
• RefSeq Size: 7124 bp
• RefSeq ORF: 4305 bp
• Locus ID: 4842
• UniProt ID: P29475
• Cytogenetics: 12q24.22
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
• Gene Summary: The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]