Frizzled 8 (FZD8) (NM_031866) Human Tagged ORF Clone

CAT#: RG222311

FZD8 (tGFP-tagged) - Human frizzled family receptor 8 (FZD8)

Product Images

Circular map for RG222311

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Frizzled 8
• Synonyms: FZ-8; hFZ8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222311 representing NM_031866
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTGGGGTTACCTGTTGGAAGTGACCTCGCTGCTGGCCGCCTTGGCGCTGCTGCAGCGCTCTAGCG
  GCGCTGCGGCCGCCTCGGCCAAGGAGCTGGCATGCCAAGAGATCACCGTGCCGCTGTGTAAGGGCATCGG
  CTACAACTACACCTACATGCCCAATCAGTTCAACCACGACACGCAAGACGAGGCGGGCCTGGAGGTGCAC
  CAGTTCTGGCCGCTGGTGGAGATCCAGTGCTCGCCCGATCTCAAGTTCTTCCTGTGCAGCATGTACACGC
  CCATCTGCCTAGAGGACTACAAGAAGCCGCTGCCGCCCTGCCGCTCGGTGTGCGAGCGCGCCAAGGCCGG
  CTGCGCGCCGCTCATGCGCCAGTACGGCTTCGCCTGGCCCGACCGCATGCGCTGCGACCGGCTGCCCGAG
  CAAGGCAACCCTGACACGCTGTGCATGGACTACAACCGCACCGACCTAACCACCGCCGCGCCCAGCCCGC
  CGCGCCGCCTGCCGCCGCCGCCGCCCGGCGAGCAGCCGCCTTCGGGCAGCGGCCACGGCCGCCCGCCGGG
  GGCCAGGCCCCCGCACCGCGGAGGCGGCAGGGGCGGTGGCGGCGGGGACGCGGCGGCGCCCCCAGCTCGC
  GGCGGCGGCGGTGGCGGGAAGGCGCGGCCCCCTGGCGGCGGCGCGGCTCCCTGCGAGCCCGGGTGCCAGT
  GCCGCGCGCCTATGGTGAGCGTGTCCAGCGAGCGCCACCCGCTCTACAACCGCGTCAAGACAGGCCAGAT
  CGCTAACTGCGCGCTGCCCTGCCACAACCCCTTTTTCAGCCAGGACGAGCGCGCCTTCACCGTCTTCTGG
  ATCGGCCTGTGGTCGGTGCTCTGCTTCGTGTCCACCTTCGCCACCGTCTCCACCTTCCTTATCGACATGG
  AGCGCTTCAAGTACCCGGAGCGGCCCATTATCTTCCTCTCGGCCTGCTACCTCTTCGTGTCGGTGGGCTA
  CCTAGTGCGCCTGGTGGCGGGCCACGAGAAGGTGGCGTGCAGCGGTGGCGCGCCGGGCGCGGGGGGCGCT
  GGGGGCGCGGGCGGCGCGGCGGCGGGCGCGGGCGCGGCGGGCGCGGGCGCGGGCGGCCCGGGCGGGCGCG
  GCGAGTACGAGGAGCTGGGCGCGGTGGAGCAGCACGTGCGCTACGAGACCACCGGCCCCGCGCTGTGCAC
  CGTGGTCTTCTTGCTGGTCTACTTCTTCGGCATGGCCAGCTCCATCTGGTGGGTGATCTTGTCGCTCACA
  TGGTTCCTGGCGGCCGGTATGAAGTGGGGCAACGAAGCCATCGCCGGCTACTCGCAGTACTTCCACCTGG
  CCGCGTGGCTTGTGCCCAGCGTCAAGTCCATCGCGGTGCTGGCGCTCAGCTCGGTGGACGGCGACCCGGT
  GGCGGGCATCTGCTACGTGGGCAACCAGAGCCTGGACAACCTGCGCGGCTTCGTGCTGGCGCCGCTGGTC
  ATCTACCTCTTCATCGGCACCATGTTCCTGCTGGCCGGCTTCGTGTCCCTGTTCCGCATCCGCTCGGTCA
  TCAAGCAACAGGACGGCCCCACCAAGACGCACAAGCTGGAGAAGCTGATGATCCGCCTGGGCCTGTTCAC
  CGTGCTCTACACCGTGCCCGCCGCGGTGGTGGTCGCCTGCCTCTTCTACGAGCAGCACAACCGCCCGCGC
  TGGGAGGCCACGCACAACTGCCCGTGCCTGCGGGACCTGCAGCCCGACCAGGCACGCAGGCCCGACTACG
  CCGTCTTCATGCTCAAGTACTTCATGTGCCTAGTGGTGGGCATCACCTCGGGCGTGTGGGTCTGGTCCGG
  CAAGACGCTGGAGTCCTGGCGCTCCCTGTGCACCCGCTGCTGCTGGGCCAGCAAGGGCGCCGCGGTGGGC
  GGGGGCGCGGGCGCCACGGCCGCGGGGGGTGGCGGCGGGCCGGGGGGCGGCGGCGGCGGGGGACCCGGCG
  GCGGCGGGGGGCCGGGCGGCGGCGGGGGCTCCCTCTACAGCGACGTCAGCACTGGCCTGACGTGGCGGTC
  GGGCACGGCGAGCTCCGTGTCTTATCCAAAGCAGATGCCATTGTCCCAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222311 representing NM_031866
  Red=Cloning site Green=Tags(s)
  
  MEWGYLLEVTSLLAALALLQRSSGAAAASAKELACQEITVPLCKGIGYNYTYMPNQFNHDTQDEAGLEVH
  QFWPLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERAKAGCAPLMRQYGFAWPDRMRCDRLPE
  QGNPDTLCMDYNRTDLTTAAPSPPRRLPPPPPGEQPPSGSGHGRPPGARPPHRGGGRGGGGGDAAAPPAR
  GGGGGGKARPPGGGAAPCEPGCQCRAPMVSVSSERHPLYNRVKTGQIANCALPCHNPFFSQDERAFTVFW
  IGLWSVLCFVSTFATVSTFLIDMERFKYPERPIIFLSACYLFVSVGYLVRLVAGHEKVACSGGAPGAGGA
  GGAGGAAAGAGAAGAGAGGPGGRGEYEELGAVEQHVRYETTGPALCTVVFLLVYFFGMASSIWWVILSLT
  WFLAAGMKWGNEAIAGYSQYFHLAAWLVPSVKSIAVLALSSVDGDPVAGICYVGNQSLDNLRGFVLAPLV
  IYLFIGTMFLLAGFVSLFRIRSVIKQQDGPTKTHKLEKLMIRLGLFTVLYTVPAAVVVACLFYEQHNRPR
  WEATHNCPCLRDLQPDQARRPDYAVFMLKYFMCLVVGITSGVWVWSGKTLESWRSLCTRCCWASKGAAVG
  GGAGATAAGGGGGPGGGGGGGPGGGGGPGGGGGSLYSDVSTGLTWRSGTASSVSYPKQMPLSQV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031866
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031866.1, NP_114072.1
• RefSeq Size: 3195 bp
• RefSeq ORF: 2085 bp
• Locus ID: 8325
• UniProt ID: Q9H461
• Cytogenetics: 10p11.21
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008]