POGK (NM_017542) Human Tagged ORF Clone

CAT#: RG222119

POGK (tGFP-tagged) - Human pogo transposable element with KRAB domain (POGK)

Product Images

Circular map for RG222119

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POGK
• Synonyms: BASS2; KRBOX2; LST003
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222119 representing NM_017542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCACAGCCTACCCTCTCAATTTGAGCCTGAAAGAAGAGGAAGAGGAAGAAGAGATTCAGAGCC
  GGGAACTAGAGGACGGCCCGGCAGACATGCAGAAAGTACGAATCTGCTCTGAGGGCGGATGGGTACCGGC
  CCTATTTGATGAGGTGGCCATATATTTTTCCGATGAGGAATGGGAAGTTTTGACGGAGCAACAAAAGGCC
  CTCTACCGGGAAGTCATGAGGATGAATTATGAAACTGTCCTGTCCCTGGAATTCCCATTCCCTAAGCCAG
  ACATGATCACCCGTTTGGAAGGGGAGGAGGAGTCTCAGAATTCTGACGAGTGGCAGCTCCAAGGAGGCAC
  CTCTGCAGAAAATGAAGAATCTGACGTAAAGCCTCCAGACTGGCCAAACCCAATGAATGCTACCTCCCAG
  TTTCCTCAGCCTCAGCACTTTGACAGCTTTGGCCTCCGTCTGCCTCGGGATATCACAGAGCTGCCCGAGT
  GGAGTGAGGGGTACCCCTTCTACATGGCCATGGGCTTCCCAGGGTATGACCTCTCGGCTGATGACATAGC
  TGGGAAGTTTCAGTTCAGCCGGGGCATGCGCCGCAGTTACGACGCAGGGTTCAAGCTGATGGTAGTGGAA
  TATGCTGAGAGTACCAACAACTGCCAGGCTGCCAAGCAGTTTGGAGTATTGGAAAAAAACGTTCGAGACT
  GGCGCAAAGTGAAGCCACAGCTTCAAAACGCCCACGCCATGCGGCGGGCATTCCGAGGCCCCAAGAATGG
  GAGGTTTGCTCTGGTGGACCAGCGTGTGGCCGAATATGTCAGATACATGCAGGCCAAAGGGGACCCCATC
  ACCCGGGAGGCGATGCAGCTGAAAGCTCTCGAAATCGCCCAGGAAATGAACATTCCAGAGAAAGGGTTCA
  AGGCAAGCTTGGGTTGGTGTCGAAGAATGATGAGAAGGTATGACCTGTCTCTGAGGCATAAAGTGCCCGT
  GCCCCAGCACCTGCCGGAAGACCTGACTGAGAAACTCGTCACTTACCAGCGCAGTGTCCTGGCTCTGCGC
  AGGGCGCATGACTATGAGGTAGCTCAGATGGGGAATGCAGATGAGACGCCCATTTGTTTAGAGGTGCCAT
  CACGGGTAACTGTTGATAACCAGGGCGAAAAGCCTGTCTTGGTCAAGACACCAGGCAGGGAAAAACTGAA
  AATCACAGCAATGCTTGGTGTCTTGGCTGATGGGAGGAAGTTACCACCGTACATCATTTTGAGGGGAACA
  TATATCCCCCCGGGGAAGTTTCCCAGTGGGATGGAAATTCGCTGCCACCGGTATGGGTGGATGACTGAAG
  ACTTGATGCAGGACTGGTTGGAAGTGGTGTGGAGACGGAGGACAGGAGCAGTGCCCAAGCAGCGAGGGAT
  GCTGATCTTGAATGGCTTCCGGGGCCATGCCACAGATTCCGTGAAGAACTCCATGGAAAGCATGAACACT
  GACATGGTGATCATCCCAGGGGGTCTGACCTCACAGCTTCAGGTGCTGGATGTCGTGGTCTACAAGCCAC
  TGAATGACAGTGTGCGGGCCCAGTACTCCAACTGGCTTCTGGCTGGGAACCTGGCGCTGAGCCCAACCGG
  GAATGCTAAGAAGCCACCCCTGGGCCTCTTTCTGGAGTGGGTCATGGTCGCGTGGAATAGCATCTCAAGT
  GAGTCCATCGTCCAAGGGTTCAAGAAGTGCCATATCTCCAGCAACTTGGAGGAGGAAGACGATGTCCTGT
  GGGAAATCGAGAGTGAGTTGCCAGGAGGAGGAGAACCACCAAAAGATTGTGACACCGAAAGCATGGCTGA
  GAGCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222119 representing NM_017542
  Red=Cloning site Green=Tags(s)
  
  MESTAYPLNLSLKEEEEEEEIQSRELEDGPADMQKVRICSEGGWVPALFDEVAIYFSDEEWEVLTEQQKA
  LYREVMRMNYETVLSLEFPFPKPDMITRLEGEEESQNSDEWQLQGGTSAENEESDVKPPDWPNPMNATSQ
  FPQPQHFDSFGLRLPRDITELPEWSEGYPFYMAMGFPGYDLSADDIAGKFQFSRGMRRSYDAGFKLMVVE
  YAESTNNCQAAKQFGVLEKNVRDWRKVKPQLQNAHAMRRAFRGPKNGRFALVDQRVAEYVRYMQAKGDPI
  TREAMQLKALEIAQEMNIPEKGFKASLGWCRRMMRRYDLSLRHKVPVPQHLPEDLTEKLVTYQRSVLALR
  RAHDYEVAQMGNADETPICLEVPSRVTVDNQGEKPVLVKTPGREKLKITAMLGVLADGRKLPPYIILRGT
  YIPPGKFPSGMEIRCHRYGWMTEDLMQDWLEVVWRRRTGAVPKQRGMLILNGFRGHATDSVKNSMESMNT
  DMVIIPGGLTSQLQVLDVVVYKPLNDSVRAQYSNWLLAGNLALSPTGNAKKPPLGLFLEWVMVAWNSISS
  ESIVQGFKKCHISSNLEEEDDVLWEIESELPGGGEPPKDCDTESMAESN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017542
• ORF Size: 1827 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017542.5
• RefSeq Size: 3852 bp
• RefSeq ORF: 1830 bp
• Locus ID: 57645
• UniProt ID: Q9P215
• Cytogenetics: 1q24.1
• Domains: KRAB, DDE, CENPB
• Protein Families: Transcription Factors
• Gene Summary: The exact function of the protein encoded by this gene is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human. [provided by RefSeq, Jul 2008]