BHLHB5 (BHLHE22) (NM_152414) Human Tagged ORF Clone

CAT#: RG222046

BHLHE22 (tGFP-tagged) - Human basic helix-loop-helix family, member e22 (BHLHE22)

Product Images

Circular map for RG222046

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BHLHB5
• Synonyms: Beta3; Beta3a; BHLHB5; CAGL85; TNRC20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222046 representing NM_152414
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCGGGATGCACCTCGGTGCAGCGGCCGCCGGCGAGGACGACCTCTTCCTGCACAAGAGCCTGA
  GCGCCTCCACCTCCAAGCGCTTGGAAGCGGCTTTCCGCTCCACGCCCCCGGGCATGGACCTGTCCCTGGC
  GCCGCCGCCTCGGGAACGCCCGGCGTCCTCCTCCTCGTCGCCCCTGGGCTGCTTCGAGCCGGCTGACCCC
  GAGGGGGCAGGGCTGCTGTTGCCGCCGCCTGGAGGAGGCGGCGGCGGCAGCGCGGGAAGTGGCGGCGGCG
  GCGGCGGCGGGGTGGGTGTCCCCGGGCTGCTAGTAGGTTCAGCCGGCGTTGGGGGCGACCCTAGCCTAAG
  CAGCCTGCCGGCCGGGGCCGCCCTTTGCCTCAAGTACGGCGAAAGCGCGAGCCGGGGCTCGGTGGCCGAG
  AGCAGCGGCGGCGAGCAGAGCCCCGACGACGACAGCGACGGTCGCTGCGAGCTCGTGCTGCGGGCCGGAG
  TAGCCGACCCGCGGGCCTCCCCGGGAGCGGGAGGTGGTGGCGCGAAGGCAGCCGAGGGCTGCTCCAATGC
  CCACCTCCACGGCGGCGCCAGCGTCCCCCCGGGGGGCCTGGGCGGCGGCGGCGGCGGGGGTAGCAGCAGC
  GGTAGCAGTGGCGGCGGTGGCGGTAGCGGTAGCGGCAGCGGCGGCAGCAGCAGCAGCAGCAGCAGCAGCA
  GCAAGAAATCCAAAGAGCAAAAGGCGCTGCGGCTTAACATCAATGCCCGAGAGCGCCGGCGGATGCACGA
  CCTGAACGACGCGCTGGACGAGCTGCGCGCGGTGATCCCCTACGCGCACAGCCCCTCGGTGCGAAAGCTC
  TCCAAGATCGCCACGCTGCTGCTCGCCAAGAACTACATCCTCATGCAGGCGCAGGCCCTGGAGGAGATGC
  GGCGCCTAGTCGCCTACCTCAACCAGGGCCAGGCCATCTCGGCTGCCTCCCTGCCCAGCTCGGCGGCTGC
  AGCGGCAGCAGCTGCTGCCCTGCACCCGGCGCTCGGCGCCTACGAGCAGGCAGCCGGCTACCCGTTCAGC
  GCCGGACTGCCCCCGGCTGCCTCCTGCCCGGAGAAGTGCGCCCTGTTTAACAGCGTCTCCTCCAGCCTCT
  GCAAACAGTGCACGGAGAAGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222046 representing NM_152414
  Red=Cloning site Green=Tags(s)
  
  MERGMHLGAAAAGEDDLFLHKSLSASTSKRLEAAFRSTPPGMDLSLAPPPRERPASSSSSPLGCFEPADP
  EGAGLLLPPPGGGGGGSAGSGGGGGGGVGVPGLLVGSAGVGGDPSLSSLPAGAALCLKYGESASRGSVAE
  SSGGEQSPDDDSDGRCELVLRAGVADPRASPGAGGGGAKAAEGCSNAHLHGGASVPPGGLGGGGGGGSSS
  GSSGGGGGSGSGSGGSSSSSSSSSKKSKEQKALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKL
  SKIATLLLAKNYILMQAQALEEMRRLVAYLNQGQAISAASLPSSAAAAAAAAALHPALGAYEQAAGYPFS
  AGLPPAASCPEKCALFNSVSSSLCKQCTEKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152414
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152414.5
• RefSeq Size: 3388 bp
• RefSeq ORF: 1146 bp
• Locus ID: 27319
• UniProt ID: Q8NFJ8
• Cytogenetics: 8q12.3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors that regulate cell fate determination, proliferation, and differentiation. A similar protein in mouse is required for the development of the dorsal cochlear nuclei, and is thought to play a role in in the differentiation of neurons involved in sensory input. The mouse protein also functions in retinogenesis. [provided by RefSeq, Oct 2016]