EVI1 (MECOM) (NM_005241) Human Tagged ORF Clone

CAT#: RG221770

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  • TrueORF®

MECOM (GFP-tagged) - Human MDS1 and EVI1 complex locus (MECOM), transcript variant 2



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,115.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol MECOM
Synonyms AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1; PRDM3; RUSAT2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG221770 representing NM_005241.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAGAGCGAAGACTATCCCCATGAAACTATGGCGCCGGATATCCACGAAGAACGGCAATATCGCTGC
GAAGACTGTGACCAGCTCTTTGAATCTAAGGCTGAACTAGCAGATCACCAAAAGTTTCCATGCAGTACT
CCTCACTCAGCATTTTCAATGGTTGAAGAGGACTTTCAGCAAAAACTCGAAAGCGAGAATGATCTCCAA
GAGATACACACGATCCAGGAGTGTAAGGAATGTGACCAAGTTTTTCCTGATTTGCAAAGCCTGGAGAAA
CACATGCTGTCACATACTGAAGAGAGGGAATACAAGTGTGATCAGTGTCCCAAGGCATTTAACTGGAAG
TCCAATTTAATTCGCCACCAGATGTCACATGACAGTGGAAAGCACTATGAATGTGAAAACTGTGCCAAG
GTTTTCACGGACCCTAGCAACCTTCAGCGGCACATTCGCTCTCAGCATGTCGGTGCCCGGGCCCATGCA
TGCCCGGAGTGTGGCAAAACGTTTGCCACTTCGTCGGGCCTCAAACAACACAAGCACATCCACAGCAGT
GTGAAGCCCTTTATCTGTGAGGTCTGCCATAAATCCTATACTCAGTTTTCAAACCTTTGCCGTCATAAG
CGCATGCATGCTGATTGCAGAACCCAAATCAAGTGCAAAGACTGTGGACAAATGTTCAGCACTACGTCT
TCCTTAAATAAACACAGGAGGTTTTGTGAGGGCAAGAACCATTTTGCGGCAGGTGGATTTTTTGGCCAA
GGCATTTCACTTCCTGGAACCCCAGCTATGGATAAAACGTCCATGGTTAATATGAGTCATGCCAACCCG
GGCCTTGCTGACTATTTTGGCGCCAATAGGCATCCTGCTGGTCTTACCTTTCCAACAGCTCCTGGATTT
TCTTTTAGCTTCCCTGGTCTGTTTCCTTCCGGCTTGTACCACAGGCCTCCTTTGATACCTGCTAGTTCT
CCTGTTAAAGGACTATCAAGTACTGAACAGACAAACAAAAGTCAAAGTCCCCTCATGACACATCCTCAG
ATACTGCCAGCTACACAGGATATTTTGAAGGCACTATCTAAACACCCATCTGTAGGGGACAATAAGCCA
GTGGAGCTCCAGCCCGAGAGGTCCTCTGAAGAGAGGCCCTTTGAGAAAATCAGTGACCAGTCAGAGAGT
AGTGACCTTGATGATGTCAGTACACCAAGTGGCAGTGACCTGGAAACAACCTCGGGCTCTGATCTGGAA
AGTGACATTGAAAGTGATAAAGAGAAATTTAAAGAAAATGGTAAAATGTTCAAAGACAAAGTAAGCCCT
CTTCAGAATCTGGCTTCAATAAATAATAAGAAAGAATACAGCAATCATTCCATTTTCTCACCATCTTTA
GAGGAGCAGACTGCGGTGTCAGGAGCTGTGAATGATTCTATAAAGGCTATTGCTTCTATTGCTGAAAAA
TACTTTGGTTCAACAGGACTGGTGGGGCTGCAAGACAAAAAAGTTGGAGCTTTACCTTACCCTTCCATG
TTTCCCCTCCCATTTTTTCCAGCATTCTCTCAATCAATGTACCCATTTCCTGATAGAGACTTGAGATCG
TTACCTTTGAAAATGGAACCCCAATCACCAGGTGAAGTAAAGAAACTGCAGAAGGGCAGCTCTGAGTCC
CCCTTTGATCTCACCACTAAGCGAAAGGATGAGAAGCCCTTGACTCCAGTCCCCTCCAAGCCTCCAGTG
ACACCTGCCACAAGCCAAGACCAGCCCCTGGATCTAAGTATGGGCAGTAGGAGTAGAGCCAGTGGGACA
AAGCTGACTGAGCCTCGAAAAAACCACGTGTTTGGGGGAAAAAAAGGAAGCAACGTCGAATCAAGACCT
GCTTCAGATGGTTCCTTGCAGCATGCAAGACCCACTCCTTTCTTTATGGACCCTATTTACAGAGTAGAG
AAAAGAAAACTAACTGACCCACTTGAAGCTTTAAAAGAGAAATACTTGAGGCCTTCTCCAGGATTCTTG
TTTCACCCACAATTCCAACTGCCTGATCAGAGAACTTGGATGTCAGCTATTGAAAACATGGCAGAAAAG
CTAGAGAGCTTCAGTGCCCTGAAACCTGAGGCCAGTGAGCTCTTACAGTCAGTGCCCTCTATGTTCAAC
TTCAGGGCGCCTCCCAATGCCCTGCCAGAGAACCTTCTGCGGAAGGGAAAGGAGCGCTATACCTGCAGA
TACTGTGGCAAGATTTTTCCAAGGTCTGCAAACCTAACACGGCACTTGAGAACCCACACAGGAGAGCAG
CCTTACAGATGCAAATACTGTGACAGATCATTTAGCATATCTTCTAACTTGCAAAGGCATGTTCGCAAC
ATCCACAATAAAGAGAAGCCATTTAAGTGTCACTTATGTGATAGGTGTTTTGGTCAACAAACCAATTTA
GACAGACACCTAAAGAAACATGAGAATGGGAACATGTCCGGTACAGCAACATCGTCGCCTCATTCTGAA
CTGGAAAGTACAGGTGCGATTCTGGATGACAAAGAAGATGCTTACTTCACAGAAATTCGAAATTTCATT
GGGAACAGCAACCATGGCAGCCAATCTCCCAGGAATGTGGAGGAGAGAATGAATGGCAGTCATTTTAAA
GATGAAAAGGCTTTGGTGACCAGTCAAAATTCAGACTTGCTGGATGATGAAGAAGTTGAAGATGAGGTG
TTGTTAGATGAGGAGGATGAAGACAATGATATTACTGGAAAAACAGGAAAGGAACCAGTGACAAGTAAT
TTACATGAAGGAAACCCTGAGGATGACTATGAAGAAACCAGTGCCCTGGAGATGAGTTGCAAGACATCC
CCAGTGAGGTATAAAGAGGAAGAATATAAAAGTGGACTTTCTGCTCTAGATCATATAAGGCACTTCACA
GATAGCCTCAAAATGAGGAAAATGGAAGATAATCAATATTCTGAAGCTGAGCTGTCTTCTTTTAGTACT
TCCCATGTGCCAGAGGAACTTAAGCAGCCGTTACACAGAAAGTCCAAATCGCAGGCATATGCTATGATG
CTGTCACTGTCTGACAAGGAGTCCCTCCATTCTACATCCCACAGTTCTTCCAACGTGTGGCACAGTATG
GCCAGGGCTGCGGCGGAATCCAGTGCTATCCAGTCCATAAGCCACGTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
>Peptide sequence encoded by RG221770
Blue=ORF Red=Cloning site Green=Tag(s)

MKSEDYPHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQ
EIHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAK
VFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHK
RMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANP
GLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQ
ILPATQDILKALSKHPSVGDNKPVELQPERSSEERPFEKISDQSESSDLDDVSTPSGSDLETTSGSDLE
SDIESDKEKFKENGKMFKDKVSPLQNLASINNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEK
YFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPGEVKKLQKGSSES
PFDLTTKRKDEKPLTPVPSKPPVTPATSQDQPLDLSMGSRSRASGTKLTEPRKNHVFGGKKGSNVESRP
ASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFQLPDQRTWMSAIENMAEK
LESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQ
PYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSE
LESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEV
LLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFT
DSLKMRKMEDNQYSEAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTSHSSSNVWHSM
ARAAAESSAIQSISHV

TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV

Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005241
ORF Size 3153 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005241.4
RefSeq Size 3570 bp
RefSeq ORF 3156 bp
Locus ID 2122
UniProt ID Q13465
Cytogenetics 3q26.2
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Chronic myeloid leukemia, MAPK signaling pathway, Pathways in cancer
MW 118.3 kDa
Gene Summary The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
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