AKAP4 (NM_003886) Human Tagged ORF Clone

CAT#: RG221765

AKAP4 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AKAP4
• Synonyms: AKAP-4; AKAP 82; AKAP82; CT99; FSC1; hAKAP82; HI; p82; PRKA4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221765 representing NM_003886
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCGTACTCTGATACTACAATGATGTCTGATGATATTGACTGGTTACGCAGCCACAGGGGTGTGT
  GCAAGGTAGATCTCTACAACCCAGAAGGACAGCAAGATCAGGACCGGAAAGTGATATGCTTTGTCGATGT
  GTCCACCCTGAATGTAGAAGATAAAGATTACAAGGATGCTGCTAGTTCCAGCTCAGAAGGCAACTTAAAC
  CTGGGAAGTCTGGAAGAAAAAGAGATTATCGTGATCAAGGACACTGAGAAGAAAGACCAGTCTAAGACAG
  AGGGATCTGTATGCCTTTTCAAACAAGCTCCCTCTGATCCTGTAAGTGTCCTCAACTGGCTTCTCAGTGA
  TCTCCAGAAGTATGCCTTGGGTTTCCAACATGCACTGAGCCCCTCAACCTCTACCTGTAAACATAAAGTA
  GGAGACACAGAGGGCGAATATCACAGAGCATCCTCTGAGAACTGCTACAGTGTCTATGCCGATCAAGTGA
  ACATAGATTATTTGATGAACAGACCTCAAAACCTACGTCTAGAAATGACAGCAGCTAAAAACACCAACAA
  TAATCAAAGTCCTTCAGCTCCTCCAGCCAAACCTCCTAGCACTCAGAGAGCAGTCATTTCCCCTGATGGA
  GAATGTTCTATAGATGACCTTTCCTTCTACGTCAACCGACTATCTTCTCTGGTAATCCAGATGGCCCATA
  AGGAAATCAAGGAGAAGTTGGAAGGTAAAAGCAAATGCCTTCATCATTCAATCTGTCCATCCCCTGGGAA
  CAAAGAGAGAATCAGTCCCCGAACTCCTGCGAGCAAGATTGCTTCTGAAATGGCCTATGAAGCTGTGGAA
  CTGACAGCTGCAGAAATGCGTGGCACTGGAGAGGAGTCCAGGGAAGGTGGCCAGAAAAGCTTTCTATATA
  GCGAATTATCCAACAAGAGCAAAAGTGGAGACAAACAGATGTCCCAGAGAGAGAGCAAAGAATTTGCAGA
  TTCCATCAGCAAGGGGCTCATGGTTTATGCAAATCAGGTGGCATCTGACATGATGGTCTCTCTCATGAAG
  ACCTTGAAAGTGCACAGCTCTGGGAAGCCAATTCCAGCATCTGTGGTCCTGAAGAGGGTGTTGCTAAGGC
  ACACCAAGGAGATTGTGTCCGATTTGATTGATTCTTGCATGAAGAACCTGCATAATATTACTGGGGTCCT
  GATGACTGACTCAGACTTTGTCTCAGCTGTCAAGAGAAATCTGTTCAACCAGTGGAAACAAAATGCTACA
  GACATCATGGAGGCCATGCTGAAGCGCTTGGTCAGTGCCCTTATAGGTGAGGAGAAGGAGACTAAGTCTC
  AGAGTCTGTCATATGCATCTTTAAAAGCTGGGTCCCATGATCCCAAATGCAGGAATCAGAGTCTTGAATT
  CTCCACCATGAAAGCTGAAATGAAAGAGAGGGACAAAGGCAAAATGAAATCAGACCCATGCAAGTCACTG
  ACTAGTGCTGAGAAAGTCGGTGAACACATTCTCAAAGAGGGCCTAACCATCTGGAACCAAAAGCAAGGAA
  ACTCATGCAAGGTGGCTACCAAAGCATGCAGCAATAAAGATGAGAAAGGAGAAAAGATCAATGCTTCCAC
  AGATTCACTGGCCAAGGACCTGATTGTCTCTGCCCTTAAGCTGATCCAGTACCATCTGACCCAGCAGACT
  AAGGGCAAAGATACATGTGAAGAAGACTGTCCTGGTTCCACCATGGGCTATATGGCTCAGAGTACTCAAT
  ATGAAAAGTGTGGAGGTGGCCAAAGTGCCAAAGCACTTTCAGTGAAACAACTAGAATCTCACAGAGCCCC
  TGGACCATCCACCTGTCAAAAGGAGAACCAACACCTGGACTCCCAGAAAATGGATATGTCAAACATCGTT
  CTAATGCTGATTCAGAAACTGCTTAATGAGAACCCCTTCAAATGTGAGGATCCATGCGAAGGTGAGAACA
  AGTGTTCTGAGCCCAGGGCAAGCAAAGCAGCTTCCATGTCCAACAGATCTGACAAAGCGGAAGAACAATG
  CCAGGAGCATCAAGAACTTGACTGTACCAGTGGGATGAAGCAAGCGAACGGGCAATTTATAGATAAACTA
  GTAGAATCTGTGATGAAGCTCTGCCTTATCATGGCTAAGTATAGCAACGATGGGGCAGCCCTTGCTGAGT
  TGGAAGAACAAGCAGCCTCGGCAAATAAGCCCAATTTCAGGGGCACCAGATGCATTCACAGTGGTGCAAT
  GCCACAGAACTATCAAGACTCTCTTGGACATGAAGTAATTGTCAATAATCAGTGCTCTACAAATAGCTTG
  CAGAAGCAGCTCCAGGCTGTCCTGCAGTGGATTGCAGCCTCCCAGTTTAACGTGCCCATGCTCTACTTCA
  TGGGAGATAAGGATGGACAACTGGAAAAGCTTCCTCAGGTTTCAGCTAAAGCAGCAGAGAAGGGGTACAG
  TGTAGGAGGTCTTCTTCAAGAGGTCATGAAGTTTGCCAAGGAACGGCAACCAGATGAAGCTGTGGGAAAG
  GTGGCCAGGAAACAGTTGCTGGACTGGCTGCTCGCTAACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221765 representing NM_003886
  Red=Cloning site Green=Tags(s)
  
  MMAYSDTTMMSDDIDWLRSHRGVCKVDLYNPEGQQDQDRKVICFVDVSTLNVEDKDYKDAASSSSEGNLN
  LGSLEEKEIIVIKDTEKKDQSKTEGSVCLFKQAPSDPVSVLNWLLSDLQKYALGFQHALSPSTSTCKHKV
  GDTEGEYHRASSENCYSVYADQVNIDYLMNRPQNLRLEMTAAKNTNNNQSPSAPPAKPPSTQRAVISPDG
  ECSIDDLSFYVNRLSSLVIQMAHKEIKEKLEGKSKCLHHSICPSPGNKERISPRTPASKIASEMAYEAVE
  LTAAEMRGTGEESREGGQKSFLYSELSNKSKSGDKQMSQRESKEFADSISKGLMVYANQVASDMMVSLMK
  TLKVHSSGKPIPASVVLKRVLLRHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNQWKQNAT
  DIMEAMLKRLVSALIGEEKETKSQSLSYASLKAGSHDPKCRNQSLEFSTMKAEMKERDKGKMKSDPCKSL
  TSAEKVGEHILKEGLTIWNQKQGNSCKVATKACSNKDEKGEKINASTDSLAKDLIVSALKLIQYHLTQQT
  KGKDTCEEDCPGSTMGYMAQSTQYEKCGGGQSAKALSVKQLESHRAPGPSTCQKENQHLDSQKMDMSNIV
  LMLIQKLLNENPFKCEDPCEGENKCSEPRASKAASMSNRSDKAEEQCQEHQELDCTSGMKQANGQFIDKL
  VESVMKLCLIMAKYSNDGAALAELEEQAASANKPNFRGTRCIHSGAMPQNYQDSLGHEVIVNNQCSTNSL
  QKQLQAVLQWIAASQFNVPMLYFMGDKDGQLEKLPQVSAKAAEKGYSVGGLLQEVMKFAKERQPDEAVGK
  VARKQLLDWLLANL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003886
• ORF Size: 2562 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003886.3
• RefSeq Size: 2881 bp
• RefSeq ORF: 2565 bp
• Locus ID: 8852
• UniProt ID: Q5JQC9
• Cytogenetics: Xp11.22
• Protein Families: Druggable Genome
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]