AKAP4 (NM_003886) Human Tagged ORF Clone

CAT#: RG221765

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  • TrueORF®

AKAP4 (GFP-tagged) - Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 1



  "NM_003886" in other vectors (4)

USD 460.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name AKAP4 (NM_003886) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol AKAP4
Synonyms AKAP-4; AKAP 82; AKAP82; CT99; FSC1; hAKAP82; HI; p82; PRKA4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Neomycin
Sequence Data
>RG221765 representing NM_003886
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCGTACTCTGATACTACAATGATGTCTGATGATATTGACTGGTTACGCAGCCACAGGGGTGTGT
GCAAGGTAGATCTCTACAACCCAGAAGGACAGCAAGATCAGGACCGGAAAGTGATATGCTTTGTCGATGT
GTCCACCCTGAATGTAGAAGATAAAGATTACAAGGATGCTGCTAGTTCCAGCTCAGAAGGCAACTTAAAC
CTGGGAAGTCTGGAAGAAAAAGAGATTATCGTGATCAAGGACACTGAGAAGAAAGACCAGTCTAAGACAG
AGGGATCTGTATGCCTTTTCAAACAAGCTCCCTCTGATCCTGTAAGTGTCCTCAACTGGCTTCTCAGTGA
TCTCCAGAAGTATGCCTTGGGTTTCCAACATGCACTGAGCCCCTCAACCTCTACCTGTAAACATAAAGTA
GGAGACACAGAGGGCGAATATCACAGAGCATCCTCTGAGAACTGCTACAGTGTCTATGCCGATCAAGTGA
ACATAGATTATTTGATGAACAGACCTCAAAACCTACGTCTAGAAATGACAGCAGCTAAAAACACCAACAA
TAATCAAAGTCCTTCAGCTCCTCCAGCCAAACCTCCTAGCACTCAGAGAGCAGTCATTTCCCCTGATGGA
GAATGTTCTATAGATGACCTTTCCTTCTACGTCAACCGACTATCTTCTCTGGTAATCCAGATGGCCCATA
AGGAAATCAAGGAGAAGTTGGAAGGTAAAAGCAAATGCCTTCATCATTCAATCTGTCCATCCCCTGGGAA
CAAAGAGAGAATCAGTCCCCGAACTCCTGCGAGCAAGATTGCTTCTGAAATGGCCTATGAAGCTGTGGAA
CTGACAGCTGCAGAAATGCGTGGCACTGGAGAGGAGTCCAGGGAAGGTGGCCAGAAAAGCTTTCTATATA
GCGAATTATCCAACAAGAGCAAAAGTGGAGACAAACAGATGTCCCAGAGAGAGAGCAAAGAATTTGCAGA
TTCCATCAGCAAGGGGCTCATGGTTTATGCAAATCAGGTGGCATCTGACATGATGGTCTCTCTCATGAAG
ACCTTGAAAGTGCACAGCTCTGGGAAGCCAATTCCAGCATCTGTGGTCCTGAAGAGGGTGTTGCTAAGGC
ACACCAAGGAGATTGTGTCCGATTTGATTGATTCTTGCATGAAGAACCTGCATAATATTACTGGGGTCCT
GATGACTGACTCAGACTTTGTCTCAGCTGTCAAGAGAAATCTGTTCAACCAGTGGAAACAAAATGCTACA
GACATCATGGAGGCCATGCTGAAGCGCTTGGTCAGTGCCCTTATAGGTGAGGAGAAGGAGACTAAGTCTC
AGAGTCTGTCATATGCATCTTTAAAAGCTGGGTCCCATGATCCCAAATGCAGGAATCAGAGTCTTGAATT
CTCCACCATGAAAGCTGAAATGAAAGAGAGGGACAAAGGCAAAATGAAATCAGACCCATGCAAGTCACTG
ACTAGTGCTGAGAAAGTCGGTGAACACATTCTCAAAGAGGGCCTAACCATCTGGAACCAAAAGCAAGGAA
ACTCATGCAAGGTGGCTACCAAAGCATGCAGCAATAAAGATGAGAAAGGAGAAAAGATCAATGCTTCCAC
AGATTCACTGGCCAAGGACCTGATTGTCTCTGCCCTTAAGCTGATCCAGTACCATCTGACCCAGCAGACT
AAGGGCAAAGATACATGTGAAGAAGACTGTCCTGGTTCCACCATGGGCTATATGGCTCAGAGTACTCAAT
ATGAAAAGTGTGGAGGTGGCCAAAGTGCCAAAGCACTTTCAGTGAAACAACTAGAATCTCACAGAGCCCC
TGGACCATCCACCTGTCAAAAGGAGAACCAACACCTGGACTCCCAGAAAATGGATATGTCAAACATCGTT
CTAATGCTGATTCAGAAACTGCTTAATGAGAACCCCTTCAAATGTGAGGATCCATGCGAAGGTGAGAACA
AGTGTTCTGAGCCCAGGGCAAGCAAAGCAGCTTCCATGTCCAACAGATCTGACAAAGCGGAAGAACAATG
CCAGGAGCATCAAGAACTTGACTGTACCAGTGGGATGAAGCAAGCGAACGGGCAATTTATAGATAAACTA
GTAGAATCTGTGATGAAGCTCTGCCTTATCATGGCTAAGTATAGCAACGATGGGGCAGCCCTTGCTGAGT
TGGAAGAACAAGCAGCCTCGGCAAATAAGCCCAATTTCAGGGGCACCAGATGCATTCACAGTGGTGCAAT
GCCACAGAACTATCAAGACTCTCTTGGACATGAAGTAATTGTCAATAATCAGTGCTCTACAAATAGCTTG
CAGAAGCAGCTCCAGGCTGTCCTGCAGTGGATTGCAGCCTCCCAGTTTAACGTGCCCATGCTCTACTTCA
TGGGAGATAAGGATGGACAACTGGAAAAGCTTCCTCAGGTTTCAGCTAAAGCAGCAGAGAAGGGGTACAG
TGTAGGAGGTCTTCTTCAAGAGGTCATGAAGTTTGCCAAGGAACGGCAACCAGATGAAGCTGTGGGAAAG
GTGGCCAGGAAACAGTTGCTGGACTGGCTGCTCGCTAACCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG221765 representing NM_003886
Red=Cloning site Green=Tags(s)

MMAYSDTTMMSDDIDWLRSHRGVCKVDLYNPEGQQDQDRKVICFVDVSTLNVEDKDYKDAASSSSEGNLN
LGSLEEKEIIVIKDTEKKDQSKTEGSVCLFKQAPSDPVSVLNWLLSDLQKYALGFQHALSPSTSTCKHKV
GDTEGEYHRASSENCYSVYADQVNIDYLMNRPQNLRLEMTAAKNTNNNQSPSAPPAKPPSTQRAVISPDG
ECSIDDLSFYVNRLSSLVIQMAHKEIKEKLEGKSKCLHHSICPSPGNKERISPRTPASKIASEMAYEAVE
LTAAEMRGTGEESREGGQKSFLYSELSNKSKSGDKQMSQRESKEFADSISKGLMVYANQVASDMMVSLMK
TLKVHSSGKPIPASVVLKRVLLRHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNQWKQNAT
DIMEAMLKRLVSALIGEEKETKSQSLSYASLKAGSHDPKCRNQSLEFSTMKAEMKERDKGKMKSDPCKSL
TSAEKVGEHILKEGLTIWNQKQGNSCKVATKACSNKDEKGEKINASTDSLAKDLIVSALKLIQYHLTQQT
KGKDTCEEDCPGSTMGYMAQSTQYEKCGGGQSAKALSVKQLESHRAPGPSTCQKENQHLDSQKMDMSNIV
LMLIQKLLNENPFKCEDPCEGENKCSEPRASKAASMSNRSDKAEEQCQEHQELDCTSGMKQANGQFIDKL
VESVMKLCLIMAKYSNDGAALAELEEQAASANKPNFRGTRCIHSGAMPQNYQDSLGHEVIVNNQCSTNSL
QKQLQAVLQWIAASQFNVPMLYFMGDKDGQLEKLPQVSAKAAEKGYSVGGLLQEVMKFAKERQPDEAVGK
VARKQLLDWLLANL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag TurboGFP
ACCN NM_003886
ORF Size 2562 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003886.2, NP_003877.2
RefSeq Size 2881 bp
RefSeq ORF 2565 bp
Locus ID 8852
Cytogenetics Xp11.22
Protein Families Druggable Genome
Gene Summary The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Citations (1)

Other Versions

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Customer Reviews 
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