UPF3B (NM_080632) Human Tagged ORF Clone

CAT#: RG221466

UPF3B (tGFP-tagged) - Human UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 1

Product Images

Circular map for RG221466

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UPF3B
• Synonyms: HUPF3B; MRX62; MRX82; MRXS14; RENT3B; UPF3BP1; UPF3BP2; UPF3BP3; Upf3p-X; UPF3X
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221466 representing NM_080632
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAAGAGAAGGAGCACAGGCCTAAGGAGAAGCGAGTAACCCTGTTAACCCCCGCCGGGGCCACAG
  GCAGCGGTGGTGGGACCTCGGGGGACAGCTCCAAGGGGGAAGATAAGCAGGATCGCAACAAGGAGAAGAA
  AGAAGCGCTGAGCAAGGTGGTAATTCGAAGATTACCTCCCACTTTGACCAAGGAGCAGCTTCAGGAACAT
  CTTCAACCTATGCCTGAGCATGATTATTTTGAGTTTTTTTCTAATGATACGAGTTTGTATCCTCATATGT
  ATGCCAGAGCATACATCAACTTTAAAAACCAAGAGGACATTATTTTGTTCAGGGATCGCTTTGATGGTTA
  TGTATTCCTTGACAATAAAGGTCAGGAATATCCCGCTATAGTAGAATTTGCACCTTTTCAAAAAGCTGCA
  AAAAAGAAGACTAAGAAAAGAGATACCAAAGTCGGGACTATCGATGATGATCCAGAATATAGAAAGTTTT
  TGGAAAGTTATGCCACAGATAATGAGAAAATGACATCTACTCCAGAGACACTGCTAGAGGAAATAGAAGC
  AAAAAATAGAGAATTAATAGCTAAAAAGACAACCCCACTTTTGAGCTTCCTGAAAAACAAGCAGAGAATG
  AGAGAAGAAAAGAGAGAAGAAAGGAGGAGGAGAGAAATAGAAAGAAAAAGACAAAGAGAAGAAGAGAGGA
  GGAAATGGAAAGAAGAAGAGAAACGAAAAAGGAAAGATATAGAAAAGCTAAAGAAGATAGACAGAATTCC
  AGAAAGGGACAAATTAAAGGATGAACCAAAGATTAAGGTACACAGGTTTCTGTTACAAGCTGTGAATCAG
  AAAAATCTGCTCAAGAAGCCAGAAAAAGGAGATGAAAAAGAATTGGACAAAAGAGAAAAAGCCAAGAAAT
  TGGACAAAGAGAATCTCAGTGATGAAAGAGCCAGTGGGCAAAGTTGTACATTGCCCAAGCGTTCTGATAG
  CGAACTTAAAGATGAAAAACCAAAGAGACCTGAAGATGAGAGCGGCAGAGACTATAGGGAGAGGGAACGG
  GAATATGAACGAGATCAGGAGCGCATACTTCGAGAAAGAGAGAGGCTGAAGCGGCAAGAAGAAGAGCGCC
  GTAGGCAGAAGGAGCGCTATGAGAAAGAGAAGACTTTTAAGAGAAAAGAAGAAGAAATGAAAAAAGAGAA
  AGACACACTTCGGGATAAAGGAAAGAAGGCTGAAAGTACAGAATCAATAGGCAGCTCAGAAAAAACTGAA
  AAGAAAGAAGAAGTGGTCAAGAGAGATCGAATAAGAAACAAGGATCGTCCAGCGATGCAGCTTTACCAAC
  CAGGAGCTCGAAGCCGAAATCGACTCTGTCCCCCTGATGACAGCACCAAGTCTGGAGATTCAGCAGCAGA
  AAGGAAGCAGGAAAGTGGTATTAGCCATAGAAAAGAAGGAGGAGAGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221466 representing NM_080632
  Red=Cloning site Green=Tags(s)
  
  MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEH
  LQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAA
  KKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRM
  REEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQ
  KNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYRERER
  EYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTE
  KKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080632
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080632.3
• RefSeq Size: 2381 bp
• RefSeq ORF: 1452 bp
• Locus ID: 65109
• UniProt ID: Q9BZI7
• Cytogenetics: Xq24
• Gene Summary: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]