CACTIN (NM_001080543) Human Tagged ORF Clone

CAT#: RG221444

CACTIN (tGFP-tagged) - Human chromosome 19 open reading frame 29 (C19orf29), transcript variant 1

Product Images

Circular map for RG221444

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CACTIN
• Synonyms: C19orf29; fSAPc; NY-REN-24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221444 representing NM_001080543
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGGGACACACGCTCGCGCTCGCGGTCCGCGGGTCGCCGGGGCCGAAGGCGGCAGAGTCAGAGCG
  GGAGCCGAAGTCGGAGCAGGAGCCATGGGCGGCGAAACCGACGGCGCCGGGAGGACGAGGGACGGCGCAG
  ACGGAGGCGGCGGAGCCGGGAGCGCAGGTCAGATTCAGAGGAAGAGCGGTGGCAGCGCTCAGGGATGCGA
  AGCCGGAGCCCCCCGCGGCCCAAGTGGCACTCAAGAGATGGGTCCTCTCAGTCGGACTCAGGAGAGGAGC
  AGTCACGGGGCCAGTGGGCTCGCCGGCGACGGCGCGCACGCTCGTGGTCTCCTAGCTCCTCAGCATCCAG
  CTCCGCGTCTCCAGGGCGATCCCAGAGCCCCCGGGCGGCCGCGGCTGCCCTGAGCCAGCAGCAGAGCCTG
  CAGGAGCGGCTGCGGCTGCGGGAGGAGCGGAAGCAGCAGGAGGAGCTGATGAAGGCCTTCGAGACGCCCG
  AGGAGAAGCGCGCACGGCGGCTGGCCAAGAAGGAGGCCAAGGAGCGCAAGAAGCGGGAGAAGATGGGCTG
  GGGTGAGGAGTACATGGGCTACACCAACACCGACAACCCCTTCGGAGACAACAACCTGCTGGGCACCTTC
  ATCTGGAATAAGGCCCTGGAGAAGAAGGGGATCAGCCACCTGGAGGAGAAGGAGCTGAAGGAGCGGAACA
  AGAGGATCCAGGAGGACAACCGGCTGGAGCTGCAGAAGGTGAAGCAGCTGCGGCTGGAGCGGGAGCGGGA
  GAAGGCCATGCGCGAGCAGGAGCTGGAGATGCTGCAGCGCGAGAAGGAGGCAGAGCACTTCAAGACATGG
  GAGGAGCAGGAGGACAACTTCCACCTCCAGCAGGCCAAGCTGCGTTCCAAGATCCGCATCCGGGACGGGC
  GGGCCAAGCCCATCGACCTGCTGGCCAAGTACATCAGCGCTGAGGATGACGATCTGGCCGTGGAGATGCA
  TGAGCCCTACACGTTCCTCAACGGCCTCACCGTGGCCGACATGGAGGACCTGCTGGAGGATATCCAGGTC
  TACATGGAGCTGGAGCAGGGCAAGAACGCCGACTTCTGGCGGGACATGACCACCATCACCGAGGACGAGA
  TCTCCAAGCTCCGCAAGCTGGAGGCCTCGGGCAAGGGGCCAGGTGAGCGCCGCGAGGGGGTCAACGCCTC
  CGTCAGCTCTGATGTGCAGTCGGTGTTCAAGGGGAAGACATACAACCAGCTGCAGGTCATCTTCCAGGGC
  ATCGAGGGCAAAATCCGCGCTGGTGGCCCCAACCTGGACATGGGCTACTGGGAGAGCCTCCTGCAGCAGC
  TTCGTGCCCACATGGCACGGGCCCGGCTGCGTGAGCGCCACCAGGACGTGCTGCGGCAGAAGCTGTACAA
  ACTGAAGCAGGAGCAGGGCGTGGAGAGCGAGCCGCTGTTCCCCATCCTCAAGCAGGAGCCCCAGTCCCCC
  AGCCGCAGCCTGGAGCCTGAGGACGCGGCGCCCACCCCGCCCGGGCCCTCCTCGGAGGGCGGCCCCGCGG
  AGGCCGAGGTGGACGGCGCGACCCCGACAGAGGGCGACGGCGACGGGGACGGTGAGGGCGAGGGCGAGGG
  CGAGGCGGTGCTCATGGAGGAGGACCTGATCCAGCAGAGCCTGGACGACTACGACGCCGGCAGGTACAGC
  CCGCGGCTGCTCACGGCGCACGAGCTGCCACTGGACGCGCACGTGCTGGAACCGGATGAGGACCTGCAGC
  GCCTGCAGCTCTCGCGCCAGCAGCTCCAGGTCACGGGAGACGCCAGCGAGAGCGCCGAGGACATCTTCTT
  CCGGCGGGCCAAGGAGGGCATGGGCCAGGACGAGGCGCAGTTCAGCGTGGAGATGCCACTCACCGGCAAG
  GCCTACCTGTGGGCCGACAAGTACCGGCCACGCAAGCCGCGCTTCTTCAACCGCGTGCACACGGGCTTCG
  AGTGGAACAAGTACAACCAGACGCACTACGACTTTGACAACCCACCGCCCAAGATCGTGCAGGGATACAA
  GTTCAACATCTTCTACCCCGACCTCATCGACAAGCGCTCCACGCCCGAGTACTTCCTGGAGGCCTGCGCC
  GACAACAAGGATTTCGCCATCCTGCGCTTCCACGCGGGGCCGCCCTACGAGGACATCGCTTTCAAGATCG
  TCAACCGCGAGTGGGAATACTCGCACCGCCACGGCTTCCGCTGCCAGTTTGCCAACGGCATCTTCCAGCT
  GTGGTTTCACTTCAAGCGCTACCGCTATCGGCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221444 representing NM_001080543
  Red=Cloning site Green=Tags(s)
  
  MGRDTRSRSRSAGRRGRRRQSQSGSRSRSRSHGRRNRRRREDEGRRRRRRRSRERRSDSEEERWQRSGMR
  SRSPPRPKWHSRDGSSQSDSGEEQSRGQWARRRRRARSWSPSSSASSSASPGRSQSPRAAAAALSQQQSL
  QERLRLREERKQQEELMKAFETPEEKRARRLAKKEAKERKKREKMGWGEEYMGYTNTDNPFGDNNLLGTF
  IWNKALEKKGISHLEEKELKERNKRIQEDNRLELQKVKQLRLEREREKAMREQELEMLQREKEAEHFKTW
  EEQEDNFHLQQAKLRSKIRIRDGRAKPIDLLAKYISAEDDDLAVEMHEPYTFLNGLTVADMEDLLEDIQV
  YMELEQGKNADFWRDMTTITEDEISKLRKLEASGKGPGERREGVNASVSSDVQSVFKGKTYNQLQVIFQG
  IEGKIRAGGPNLDMGYWESLLQQLRAHMARARLRERHQDVLRQKLYKLKQEQGVESEPLFPILKQEPQSP
  SRSLEPEDAAPTPPGPSSEGGPAEAEVDGATPTEGDGDGDGEGEGEGEAVLMEEDLIQQSLDDYDAGRYS
  PRLLTAHELPLDAHVLEPDEDLQRLQLSRQQLQVTGDASESAEDIFFRRAKEGMGQDEAQFSVEMPLTGK
  AYLWADKYRPRKPRFFNRVHTGFEWNKYNQTHYDFDNPPPKIVQGYKFNIFYPDLIDKRSTPEYFLEACA
  DNKDFAILRFHAGPPYEDIAFKIVNREWEYSHRHGFRCQFANGIFQLWFHFKRYRYRR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080543
• ORF Size: 2274 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080543.2
• RefSeq Size: 3625 bp
• RefSeq ORF: 2277 bp
• Locus ID: 58509
• UniProt ID: Q8WUQ7
• Cytogenetics: 19p13.3
• Gene Summary: Involved in the regulation of innate immune response. Acts as negative regulator of Toll-like receptor and interferon-regulatory factor (IRF) signaling pathways. Contributes to the regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous proinflammatory stimuli. May play a role during early embryonic development. Probably involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]