GTDC1 (NM_001006636) Human Tagged ORF Clone

CAT#: RG221336

GTDC1 (tGFP-tagged) - Human glycosyltransferase-like domain containing 1 (GTDC1), transcript variant 1

Product Images

Circular map for RG221336

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GTDC1
• Synonyms: Hmat-Xa; mat-Xa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221336 representing NM_001006636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTATCCTCATCATTGAAGCATTCTATGGAGGCTCCCATAAACAGCTGGTGGATCTTCTTCAAGAAG
  AGTTAGGAGACTGTGTCGTTTATACCCTTCCTGCAAAGAAATGGCATTGGAGAGCCCGGACATCTGCTTT
  ATATTTCTCTCAGACCATTCCCATCAGTGAGCATTACAGGACCCTCTTTGCAAGTTCAGTGCTTAACCTG
  ACCGAACTGGCTGCCCTTCGGCCTGACCTTGGGAAACTGAAAAAGATTCTGTATTTTCACGAGAACCAGT
  TGATATATCCTGTCAAGAAATGTCAGGAGAGGGATTTCCAATATGGATACAACCAAATTCTTTCATGCCT
  GGTGGCTGATGTGGTTGTATTCAACTCAGTTTTTAATATGGAATCATTTCTCACTTCCATGGGAAAATTT
  ATGAAGCTGATTCCTGATCACAGACCCAAGGATCTGGAAAGCATCATCAGACCCAAGTGCCAAGTTATTT
  ACTTTCCCATCAGGTTTCCTGATGTGAGCAGATTCATGCCCAAGCACAAAACAACCCATTTAAAGAAGAT
  GCTCGGCCTTAAAGGAAATGGCGGTGCGGTTCTGTCCATGGCCCTTCCTTTTCAGCCAGAGCAGAGAGAT
  TCAGAGGATTTATTGAAGAATTTTAATTCAGAGTGTGATACACACTGTGGCCTTGATACTGCACGACAAG
  AATATTTGGGTAACTCATTAAGACAGGAATCAGACTTGAAAAAATCCACCTCGTCAGATAATTCAAGCTC
  TCATCATGGTGAAAATAAACAAAATCTGACTGTTGATCCCTGTGACATTTTGGGTGGAGTTGATAATCAG
  CAAAGACTGCTACACATTGTCTGGCCTCACAGGTGGGAGCATGATAAAGATCCAGAAAGCTTTTTTAAGG
  TATTAATGCATCTTAAAGACTTAGGACTCAATTTCCACGTGTCTGTACTTGGAGAAACCTTCACAGATGT
  CCCAGATATTTTTTCAGAGGCCAAAAAGGCATTGGGATCTTCTGTCTTACACTGGGGCTACTTACCCAGC
  AAAGATGACTATTTCCAAGTACTGTGCATGGCTGATGTTGTCATCTCAACAGCTAAGCATGAATTCTTTG
  GAGTGGCAATGTTGGAAGCTGTGTACTGTGGGTGTTACCCACTTTGTCCTAAAGATTTGGTTTATCCCGA
  AATATTTCCAGCTGAATATCTGTATTCTACACCTGAACAGCTTTCAAAAAGGCTCCAGAATTTCTGCAAG
  AGACCAGATATTATAAGAAAACATCTCTATAAGGGTGAAATCGCTCCGTTTTCTTGGGCAGCCCTACATG
  GTAAATTCAGGTCTCTGCTTACAACAGAACCTAGGGAAGATTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221336 representing NM_001006636
  Red=Cloning site Green=Tags(s)
  
  MSILIIEAFYGGSHKQLVDLLQEELGDCVVYTLPAKKWHWRARTSALYFSQTIPISEHYRTLFASSVLNL
  TELAALRPDLGKLKKILYFHENQLIYPVKKCQERDFQYGYNQILSCLVADVVVFNSVFNMESFLTSMGKF
  MKLIPDHRPKDLESIIRPKCQVIYFPIRFPDVSRFMPKHKTTHLKKMLGLKGNGGAVLSMALPFQPEQRD
  SEDLLKNFNSECDTHCGLDTARQEYLGNSLRQESDLKKSTSSDNSSSHHGENKQNLTVDPCDILGGVDNQ
  QRLLHIVWPHRWEHDKDPESFFKVLMHLKDLGLNFHVSVLGETFTDVPDIFSEAKKALGSSVLHWGYLPS
  KDDYFQVLCMADVVISTAKHEFFGVAMLEAVYCGCYPLCPKDLVYPEIFPAEYLYSTPEQLSKRLQNFCK
  RPDIIRKHLYKGEIAPFSWAALHGKFRSLLTTEPREDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001006636
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001006636.5
• RefSeq Size: 2692 bp
• RefSeq ORF: 1377 bp
• Locus ID: 79712
• UniProt ID: Q4AE62
• Cytogenetics: 2q22.3