RBMS1 (NM_016839) Human Tagged ORF Clone

CAT#: RG221296

RBMS1 (tGFP-tagged) - Human RNA binding motif, single stranded interacting protein 1 (RBMS1), transcript variant 2

Product Images

Circular map for RG221296

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RBMS1
• Synonyms: YC1, MSSP, SCR2, MSSP-1, MSSP-2, MSSP-3, MGC3331, MGC15146
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221296 representing NM_016839
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCTCCCAGTCCCAGCACCACCAGCAGTAATAACAACAGTAGCAGCAGTAGCAACTCAGGATGGG
  ATCAGCTCAGCAAAACGAACCTCTATATCCGAGGACTGCCTCCCCACACCACCGACCAGGACCTGGTGAA
  GCTCTGTCAACCATATGGGAAAATAGTCTCCACAAAGGCAATTTTGGATAAGACAACGAACAAATGCAAA
  GGTTATGGTTTTGTCGACTTTGACAGCCCTGCAGCAGCTCAAAAAGCTGTGTCTGCCCTGAAGGCCAGTG
  GGGTTCAAGCTCAAATGGCAAAGCAACAGGAACAAGATCCTACCAACCTCTACATTTCTAATTTGCCACT
  CTCCATGGATGAGCAAGAACTAGAAAATATGCTCAAACCATTTGGACAAGTTATTTCTACAAGGATACTA
  CGTGATTCCAGTGGTACAAGTCGTGGTGTTGGCTTTGCTAGGATGGAATCAACAGAAAAATGTGAAGCTG
  TTATTGGTCATTTTAATGGAAAATTTATTAAGACACCACCAGGAGTTTCTGCCCCCACAGAACCTTTATT
  GTGTAAGTTTGCTGATGGAGGACAGAAAAAGAGACAGAACCCAAACAAATACATCCCTAATGGAAGACCA
  TGGCATAGAGAAGGAGAGGTGAGACTTGCTGGAATGACACTTACTTACGACCCAACTACAGCTGCTATAC
  AGAACGGATTTTATCCTTCACCATACAGTATTGCTACAAACCGAATGATCACTCAAACTTCTATTACACC
  CTATATTGCATCTCCTGTATCTGCCTACCAGGTGGCAAAGGAAACCAGAGAAAACAAGTATCGGGGCTCT
  GCTATCAAGGTGCAAAGTCCTTCGTGGATGCAACCTCAACCATATATTCTACAGCACCCTGGTGCCGTGT
  TAACTCCCTCAATGGAGCACACCATGTCACTACAGCCCGCATCAATGATCAGCCCTCTGGCCCAGCAGAT
  GAGTCATCTGTCACTAGGCAGCACCGGAACATACATGCCTGCAACGTCAGCTATGCAAGGAGCCTACTTG
  CCACAGTATGCACATATGCAGACGACAGCGGTTCCTGTTGAGGAGGCAAGTGGTCAACAGCAGGTGGCTG
  TCGAGACGTCTAATGACCATTCTCCATATACCTTTCAACCTAATAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221296 representing NM_016839
  Red=Cloning site Green=Tags(s)
  
  MAPPSPSTTSSNNNSSSSSNSGWDQLSKTNLYIRGLPPHTTDQDLVKLCQPYGKIVSTKAILDKTTNKCK
  GYGFVDFDSPAAAQKAVSALKASGVQAQMAKQQEQDPTNLYISNLPLSMDEQELENMLKPFGQVISTRIL
  RDSSGTSRGVGFARMESTEKCEAVIGHFNGKFIKTPPGVSAPTEPLLCKFADGGQKKRQNPNKYIPNGRP
  WHREGEVRLAGMTLTYDPTTAAIQNGFYPSPYSIATNRMITQTSITPYIASPVSAYQVAKETRENKYRGS
  AIKVQSPSWMQPQPYILQHPGAVLTPSMEHTMSLQPASMISPLAQQMSHLSLGSTGTYMPATSAMQGAYL
  PQYAHMQTTAVPVEEASGQQQVAVETSNDHSPYTFQPNK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016839
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016839.2, NP_058523.1
• RefSeq Size: 2596 bp
• RefSeq ORF: 1169 bp
• Locus ID: 5937
• Cytogenetics: 2q24.2
• Gene Summary: This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. Several transcript variants, resulting from alternative splicing and encoding different isoforms, have been described. A pseudogene for this locus is found on chromosome 12. [provided by RefSeq, Feb 2009]