Hepsin (HPN) (NM_182983) Human Tagged ORF Clone

CAT#: RG221292

HPN (tGFP-tagged) - Human hepsin (HPN), transcript variant 1

Product Images

Circular map for RG221292

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hepsin
• Synonyms: TMPRSS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221292 representing NM_182983
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGAAGGAGGGTGGCCGGACTGTGCCATGCTGCTCCAGACCCAAGGTGGCAGCTCTCACTGCGG
  GGACCCTGCTACTTCTGACAGCCATCGGGGCGGCATCCTGGGCCATTGTGGCTGTTCTCCTCAGGAGTGA
  CCAGGAGCCGCTGTACCCAGTGCAGGTCAGCTCTGCGGACGCTCGGCTCATGGTCTTTGACAAGACGGAA
  GGGACGTGGCGGCTGCTGTGCTCCTCGCGCTCCAACGCCAGGGTAGCCGGACTCAGCTGCGAGGAGATGG
  GCTTCCTCAGGGCACTGACCCACTCCGAGCTGGACGTGCGAACGGCGGGCGCCAATGGCACGTCGGGCTT
  CTTCTGTGTGGACGAGGGGAGGCTGCCCCACACCCAGAGGCTGCTGGAGGTCATCTCCGTGTGTGATTGC
  CCCAGAGGCCGTTTCTTGGCCGCCATCTGCCAAGACTGTGGCCGCAGGAAGCTGCCCGTGGACCGCATCG
  TGGGAGGCCGGGACACCAGCTTGGGCCGGTGGCCGTGGCAAGTCAGCCTTCGCTATGATGGAGCACACCT
  CTGTGGGGGATCCCTGCTCTCCGGGGACTGGGTGCTGACAGCCGCCCATTGCTTCCCGGAGCGGAACCGG
  GTCCTGTCCCGATGGCGAGTGTTTGCCGGTGCCGTGGCCCAGGCCTCTCCCCACGGTCTGCAGCTGGGGG
  TGCAGGCTGTGGTCTACCACGGGGGCTATCTTCCCTTTCGGGACCCCAACAGCGAGGAGAACAGCAACGA
  TATTGCCCTGGTCCACCTCTCCAGTCCCCTGCCCCTCACAGAATACATCCAGCCTGTGTGCCTCCCAGCT
  GCCGGCCAGGCCCTGGTGGATGGCAAGATCTGTACCGTGACGGGCTGGGGCAACACGCAGTACTATGGCC
  AACAGGCCGGGGTACTCCAGGAGGCTCGAGTCCCCATAATCAGCAATGATGTCTGCAATGGCGCTGACTT
  CTATGGAAACCAGATCAAGCCCAAGATGTTCTGTGCTGGCTACCCCGAGGGTGGCATTGATGCCTGCCAG
  GGCGACAGCGGTGGTCCCTTTGTGTGTGAGGACAGCATCTCTCGGACGCCACGTTGGCGGCTGTGTGGCA
  TTGTGAGTTGGGGCACTGGCTGTGCCCTGGCCCAGAAGCCAGGCGTCTACACCAAAGTCAGTGACTTCCG
  GGAGTGGATCTTCCAGGCCATAAAGACTCACTCCGAAGCCAGCGGCATGGTGACCCAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221292 representing NM_182983
  Red=Cloning site Green=Tags(s)
  
  MAQKEGGRTVPCCSRPKVAALTAGTLLLLTAIGAASWAIVAVLLRSDQEPLYPVQVSSADARLMVFDKTE
  GTWRLLCSSRSNARVAGLSCEEMGFLRALTHSELDVRTAGANGTSGFFCVDEGRLPHTQRLLEVISVCDC
  PRGRFLAAICQDCGRRKLPVDRIVGGRDTSLGRWPWQVSLRYDGAHLCGGSLLSGDWVLTAAHCFPERNR
  VLSRWRVFAGAVAQASPHGLQLGVQAVVYHGGYLPFRDPNSEENSNDIALVHLSSPLPLTEYIQPVCLPA
  AGQALVDGKICTVTGWGNTQYYGQQAGVLQEARVPIISNDVCNGADFYGNQIKPKMFCAGYPEGGIDACQ
  GDSGGPFVCEDSISRTPRWRLCGIVSWGTGCALAQKPGVYTKVSDFREWIFQAIKTHSEASGMVTQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182983
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182983.1, NP_892028.1
• RefSeq Size: 2363 bp
• RefSeq ORF: 1254 bp
• Locus ID: 3249
• UniProt ID: P05981
• Cytogenetics: 19q13.11
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: This gene encodes a type II transmembrane serine protease that may be involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Expression of the encoded protein is associated with the growth and progression of cancers, particularly prostate cancer. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]