TAF6L (NM_006473) Human Tagged ORF Clone

CAT#: RG220716

TAF6L (tGFP-tagged) - Human TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa (TAF6L)

Product Images

Circular map for RG220716

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TAF6L
• Synonyms: PAF65A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220716 representing NM_006473
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGCGAGAAGAGCGGCGGTTTGTGGAGATCCCTCGGGAGTCTGTCCGGCTCATGGCGGAGAGCA
  CGGGCCTGGAGCTGAGCGATGAGGTGGCGGCGCTGCTCGCAGAGGACGTGTGCTATCGTCTGAGAGAGGC
  CACGCAGAATAGCTCTCAGTTCATGAAGCACACCAAACGCCGGAAGCTGACGGTTGAGGACTTCAACAGG
  GCCCTCAGATGGAGCAGCGTGGAGGCTGTGTGTGGTTACGGATCACAGGAGGCACTGCCCATGCGCCCCG
  CCAGGGAGGGTGAACTCTACTTTCCTGAGGATCGAGAGGTGAACCTGGTGGAGCTGGCCCTGGCTACCAA
  CATCCCCAAAGGCTGTGCTGAGACAGCTGTCAGAGTTCATGTCTCCTACCTGGATGGCAAAGGGAACCTG
  GCACCTCAAGGATCGGTGCCCAGTGCTGTGTCTTCACTGACAGATGACCTTCTCAAGTACTATCACCAGG
  TGACTCGTGCTGTGCTAGGGGATGATCCGCAACTGATGAAGGTTGCACTCCAGGACTTGCAGACGAACTC
  CAAGATTGGGGCACTCCTGCCTTACTTTGTTTATGTGGTCAGTGGGGTGAAATCTGTAAGCCATGACCTG
  GAGCAACTGCACCGGCTGCTGCAGGTGGCACGGAGCCTATTTCGTAATCCGCACCTGTGCTTGGGGCCCT
  ATGTCCGCTGTCTGGTGGGCAGTGTCCTCTACTGTGTCCTGGAGCCACTGGCTGCCTCCATCAACCCCCT
  GAATGACCACTGGACTCTGCGGGATGGGGCTGCCCTCCTGCTCAGCCACATCTTCTGGACTCATGGGGAC
  CTTGTAAGTGGCCTCTATCAGCATATCCTGCTATCCCTGCAGAAGATCCTGGCAGATCCTGTGCGGCCGC
  TCTGCTGCCACTATGGAGCCGTGGTGGGGCTGCATGCTCTTGGCTGGAAGGCAGTAGAACGAGTCCTGTA
  CCCACACCTGTCCACCTACTGGACAAACTTGCAGGCTGTGCTGGATGATTATTCAGTATCTAATGCCCAG
  GTCAAAGCAGATGGACACAAAGTCTATGGAGCCATTCTGGTGGCGGTAGAGCGACTGCTGAAGATGAAGG
  CCCAGGCAGCAGAGCCCAACAGGGGTGGCCCAGGTGGCAGGGGGTGCCGGCGCCTGGACGACCTGCCATG
  GGACAGCCTTCTCTTTCAAGAGTCGTCCTCCGGGGGCGGTGCAGAACCCAGCTTTGGGTCCGGCCTCCCG
  CTGCCGCCAGGGGGCGCGGGGCCGGAGGACCCTTCTCTTTCGGTGACCCTGGCCGACATCTACCGGGAGC
  TCTACGCCTTCTTCGGTGACAGCTTGGCCACACGCTTTGGCACCGGCCAGCCTGCACCCACGGCTCCGCG
  GCCGCCCGGGGACAAGAAGGAGCCGGCGGCAGCCCCGGACTCGGTGCGGAAGATGCCGCAGCTGACGGCA
  AGCGCCATAGTCAGCCCGCACGGCGACGAGAGCCCCCGGGGCAGCGGCGGAGGCGGCCCCGCGTCGGCCT
  CTGGGCCCGCCGCCTCTGAGAGCAGGCCCTTGCCGCGCGTGCATCGGGCGCGCGGGGCACCCCGGCAGCA
  GGGCCCCGGGACCGGCACCCGCGACGTTTTCCAGAAGAGCCGTTTCGCCCCGCGCGGCGCCCCGCACTTT
  CGTTTCATCATAGCCGGGCGGCAGGCTGGGAGGCGCTGCCGCGGGCGCCTTTTCCAGACTGCCTTCCCCG
  CGCCGTACGGGCCTAGCCCGGCCTCGCGCTACGTGCAGAAACTGCCCATGATCGGCCGTACCAGCCGCCC
  CGCCCGCCGGTGGGCGCTCTCGGACTACTCGCTGTACTTGCCGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220716 representing NM_006473
  Red=Cloning site Green=Tags(s)
  
  MSEREERRFVEIPRESVRLMAESTGLELSDEVAALLAEDVCYRLREATQNSSQFMKHTKRRKLTVEDFNR
  ALRWSSVEAVCGYGSQEALPMRPAREGELYFPEDREVNLVELALATNIPKGCAETAVRVHVSYLDGKGNL
  APQGSVPSAVSSLTDDLLKYYHQVTRAVLGDDPQLMKVALQDLQTNSKIGALLPYFVYVVSGVKSVSHDL
  EQLHRLLQVARSLFRNPHLCLGPYVRCLVGSVLYCVLEPLAASINPLNDHWTLRDGAALLLSHIFWTHGD
  LVSGLYQHILLSLQKILADPVRPLCCHYGAVVGLHALGWKAVERVLYPHLSTYWTNLQAVLDDYSVSNAQ
  VKADGHKVYGAILVAVERLLKMKAQAAEPNRGGPGGRGCRRLDDLPWDSLLFQESSSGGGAEPSFGSGLP
  LPPGGAGPEDPSLSVTLADIYRELYAFFGDSLATRFGTGQPAPTAPRPPGDKKEPAAAPDSVRKMPQLTA
  SAIVSPHGDESPRGSGGGGPASASGPAASESRPLPRVHRARGAPRQQGPGTGTRDVFQKSRFAPRGAPHF
  RFIIAGRQAGRRCRGRLFQTAFPAPYGPSPASRYVQKLPMIGRTSRPARRWALSDYSLYLPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006473
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006473.4
• RefSeq Size: 2176 bp
• RefSeq ORF: 1869 bp
• Locus ID: 10629
• UniProt ID: Q9Y6J9
• Cytogenetics: 11q12.3
• Domains: TAF
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is a component of the PCAF histone acetylase complex and structurally similar to one of the histone-like TAFs, TAF6. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. [provided by RefSeq, Jul 2008]