KLC4 (NM_201523) Human Tagged ORF Clone

CAT#: RG220554

KLC4 (tGFP-tagged) - Human kinesin light chain 4 (KLC4), transcript variant 3

Product Images

Circular map for RG220554

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLC4
• Synonyms: bA387M24.3; KNSL8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220554 representing NM_201523
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGACTGGGTTTGGAGTGACCAGACCGGGCAAGGTCCCCCAGGCCAGGATGTCAGGCCTGGTGT
  TGGGGCAGCGGGATGAGCCTGCAGGCCACCGGCTCAGCCAAGAGGAGATCCTGGGGAGCACACGGCTGGT
  CAGCCAAGGGCTAGAGGCCCTACGCAGTGAACACCAGGCCGTGCTGCAAAGCCTGTCCCAGACCATTGAG
  TGTCTGCAGCAGGGAGGCCATGAGGAAGGGCTGGTGCATGAGAAGGCCCGGCAGCTTCGCCGTTCTATGG
  AAAACATTGAGCTCGGGCTGAGTGAGGCCCAGGTGATGCTGGCTCTAGCCAGCCACCTGAGCACAGTGGA
  GTCGGAGAAACAGAAGCTGCGGGCTCAGGTGCGGCGGCTATGCCAGGAGAACCAGTGGCTGCGGGATGAG
  CTGGCTGGCACCCAGCAGCGGCTACAGCGCAGTGAACAGGCTGTGGCTCAGCTGGAGGAGGAAAAGAAGC
  ACCTGGAGTTCCTGGGGCAGCTGCGGCAGTATGATGAGGATGGACATACCTCGGAGGAGAAAGAAGGCGA
  TGCCACCAAGGATTCCCTGGATGACCTCTTTCCTAATGAGGAGGAAGAGGACCCCAGCAATGGCTTGTCC
  CGTGGTCAAGGTGCTACAGCAGCTCAGCAGGGTGGATATGAGATCCCAGCAAGGTTGCGGACGTTGCACA
  ACCTGGTGATCCAGTACGCAGCCCAAGGTCGCTATGAGGTGGCCGTGCCACTCTGTAAGCAGGCACTAGA
  GGACCTGGAGCGCACATCAGGCCGTGGCCACCCTGATGTCGCCACCATGCTCAACATCCTTGCTTTGGTG
  TATCGTGACCAGAATAAGTATAAGGAAGCTGCCCACCTGCTGAATGATGCCCTTAGCATCCGGGAGAGCA
  CCTTGGGACCTGACCATCCTGCTGTGGCTGCCACACTCAACAATTTGGCTGTGCTCTATGGCAAAAGGGG
  CAAGTACAAGGAGGCAGAGCCTCTGTGCCAGCGGGCACTGGAGATTCGAGAAAAGGTCCTGGGCACGAAT
  CATCCAGATGTGGCAAAACAGCTGAACAACCTGGCCCTCTTGTGCCAAAACCAGGGCAAGTATGAGGCCG
  TGGAACGCTACTACCAGCGAGCACTGGCCATCTACGAGGGGCAGCTGGGGCCGGACAACCCTAATGTAGC
  CCGGACCAAGAACAACCTGGCTTCCTGTTACCTGAAACAGGGCAAATATGCTGAGGCTGAGACACTATAC
  AAAGAGATCCTGACCCGTGCCCATGTACAGGAGTTTGGGTCTGTGGATGATGACCACAAGCCCATCTGGA
  TGCATGCAGAGGAGCGGGAGGAAATGAGCAAAAGCCGGCACCATGAGGGTGGGACACCCTATGCTGAGTA
  TGGAGGCTGGTACAAGGCCTGCAAAGTGAGCAGCCCCACAGTGAACACTACTCTGAGAAACCTGGGAGCT
  CTGTATAGGCGCCAGGGAAAGCTGGAGGCTGCTGAGACCCTGGAGGAATGTGCCCTGCGGTCCCGGAGAC
  AGGGCACTGACCCTATCAGCCAGACGAAGGTGGCAGAGCTGCTTGGGGAGAGTGATGGTAGAAGGACCTC
  CCAGGAGGGCCCTGGAGACAGTGTGAAATTCGAGGGAGGTGAAGATGCTTCTGTGGCTGTGGAGTGGTCC
  GGGGATGGCAGTGGGACCCTGCAGAGGAGTGGCTCTCTTGGCAAGATCCGGGATGTGCTCCGCAGAAGCA
  GTGAACTCTTGGTGAGGAAGCTCCAGGGGACTGAGCCTCGGCCCTCCAGCAGCAACATGAAGCGAGCAGC
  CTCCTTGAACTATCTGAACCAACCTAGTGCAGCACCCCTCCAGGTCTCCCGGGGCCTCAGTGCCAGCACC
  ATGGACCTCTCTTCAAGCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220554 representing NM_201523
  Red=Cloning site Green=Tags(s)
  
  MEVTGFGVTRPGKVPQARMSGLVLGQRDEPAGHRLSQEEILGSTRLVSQGLEALRSEHQAVLQSLSQTIE
  CLQQGGHEEGLVHEKARQLRRSMENIELGLSEAQVMLALASHLSTVESEKQKLRAQVRRLCQENQWLRDE
  LAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLS
  RGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSGRGHPDVATMLNILALV
  YRDQNKYKEAAHLLNDALSIRESTLGPDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTN
  HPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYEGQLGPDNPNVARTKNNLASCYLKQGKYAEAETLY
  KEILTRAHVQEFGSVDDDHKPIWMHAEEREEMSKSRHHEGGTPYAEYGGWYKACKVSSPTVNTTLRNLGA
  LYRRQGKLEAAETLEECALRSRRQGTDPISQTKVAELLGESDGRRTSQEGPGDSVKFEGGEDASVAVEWS
  GDGSGTLQRSGSLGKIRDVLRRSSELLVRKLQGTEPRPSSSNMKRAASLNYLNQPSAAPLQVSRGLSAST
  MDLSSSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201523
• ORF Size: 1911 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201523.3
• RefSeq Size: 2674 bp
• RefSeq ORF: 1914 bp
• Locus ID: 89953
• UniProt ID: Q9NSK0
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome
• Gene Summary: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity (By similarity).[UniProtKB/Swiss-Prot Function]