FOXD1 (NM_004472) Human Tagged ORF Clone

CAT#: RG220504

  • TrueORF®

FOXD1 (tGFP-tagged) - Human forkhead box D1 (FOXD1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_004472" in other vectors (6)

Reconstitution Protocol

USD 886.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol FOXD1
Synonyms FKHL8; FREAC-4; FREAC4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG220504 representing NM_004472
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGAGCACTGAGATGTCCGATGCCTCTGGCCTCGCCGAGGAAACAGACATCGACGTGGTGGGGG
AGGGCGAGGACGAAGAAGACGAGGAAGAGGAGGACGACGACGAGGGCGGCGGTGGCGGGCCCCGGCTGGC
TGTCCCCGCGCAGCGGCGGCGGCGGCGGCGCTCGTACGCCGGGGAGGACGAGCTGGAGGATCTGGAGGAG
GAGGAGGACGACGATGACATCCTGCTGGCCCCGCCTGCTGGGGGCTCCCCGGCGCCCCCGGGCCCGGCCC
CGGCGGCGGGGGCAGGAGCCGGTGGGGGCGGCGGCGGCGGCGGCGCGGGCGGCGGCGGGAGCGCGGGTAG
CGGCGCCAAGAACCCGCTGGTGAAGCCGCCCTACTCGTATATCGCGCTCATCACTATGGCCATCCTGCAG
AGCCCCAAGAAGCGGCTGACGCTGAGCGAGATCTGTGAGTTCATCAGCGGCCGCTTCCCCTACTACCGGG
AGAAGTTCCCCGCCTGGCAGAACAGCATCCGCCACAACCTCTCGCTCAACGACTGCTTCGTCAAGATCCC
CCGCGAGCCCGGCAACCCGGGCAAGGGCAACTACTGGACGCTGGACCCGGAGTCCGCCGACATGTTCGAC
AACGGCAGCTTCCTGCGCCGGAGGAAGCGCTTCAAGCGGCAGCCGCTGCTCCCACCCAACGCCGCGGCCG
CCGAGTCTCTGCTGCTGCGCGGCGCGGGAGCCGCAGGGGGCGCGGGCGACCCGGCAGCCGCCGCCGCGCT
CTTCCCGCCCGCGCCCCCGCCGCCCCCGCATGCCTACGGCTACGGCCCCTACGGCTGCGGCTACGGCCTG
CAGCTGCCGCCTTACGCGCCGCCCTCGGCCCTCTTCGCCGCCGCAGCGGCCGCCGCCGCCGCCGCCGCCT
TCCACCCGCACTCGCCCCCGCCGCCCCCGCCACCGCACGGCGCGGCCGCCGAGCTGGCCCGGACCGCCTT
CGGCTACCGGCCGCACCCGCTCGGCGCCGCCCTACCCGGCCCCCTGCCGGCCTCCGCGGCCAAGGCGGGC
GGCCCGGGCGCCTCAGCGCTGGCGCGCTCGCCCTTCTCCATCGAGAGCATCATCGGGGGCAGCTTGGGCC
CGGCCGCCGCTGCCGCCGCCGCCGCGCAGGCCGCCGCCGCCGCTCAGGCCTCGCCCTCGCCCTCGCCGGT
GGCGGCGCCGCCAGCTCCCGGATCCAGCGGAGGAGGCTGCGCGGCGCAGGCGGCCGTGGGCCCGGCGGCC
GCGCTCACCCGATCCCTCGTGGCCGCCGCGGCCGCCGCCGCCTCCTCAGTCTCCTCGTCCGCCGCCTTGG
GGACTCTGCACCAAGGGACTGCCCTGTCCAGTGTCGAGAACTTTACTGCTAGGATTTCCAATTGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG220504 representing NM_004472
Red=Cloning site Green=Tags(s)

MTLSTEMSDASGLAEETDIDVVGEGEDEEDEEEEDDDEGGGGGPRLAVPAQRRRRRRSYAGEDELEDLEE
EEDDDDILLAPPAGGSPAPPGPAPAAGAGAGGGGGGGGAGGGGSAGSGAKNPLVKPPYSYIALITMAILQ
SPKKRLTLSEICEFISGRFPYYREKFPAWQNSIRHNLSLNDCFVKIPREPGNPGKGNYWTLDPESADMFD
NGSFLRRRKRFKRQPLLPPNAAAAESLLLRGAGAAGGAGDPAAAAALFPPAPPPPPHAYGYGPYGCGYGL
QLPPYAPPSALFAAAAAAAAAAAFHPHSPPPPPPPHGAAAELARTAFGYRPHPLGAALPGPLPASAAKAG
GPGASALARSPFSIESIIGGSLGPAAAAAAAAQAAAAAQASPSPSPVAAPPAPGSSGGGCAAQAAVGPAA
ALTRSLVAAAAAAASSVSSSAALGTLHQGTALSSVENFTARISNC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004472
ORF Size 1395 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004472.3
RefSeq Size 2272 bp
RefSeq ORF 1398 bp
Locus ID 2297
UniProt ID Q16676
Cytogenetics 5q13.2
Protein Families Transcription Factors
Gene Summary This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Studies of the orthologous mouse protein indicate that it functions in kidney development by promoting nephron progenitor differentiation, and it also functions in the development of the retina and optic chiasm. It may also regulate inflammatory reactions and prevent autoimmunity. [provided by RefSeq, Apr 2014]

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