CD30 (TNFRSF8) (NM_001243) Human Tagged ORF Clone

CAT#: RG219819

TNFRSF8 (tGFP-tagged) - Human tumor necrosis factor receptor superfamily, member 8 (TNFRSF8), transcript variant 1

Product Images

Circular map for RG219819

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CD30
• Synonyms: CD30; D1S166E; Ki-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219819 representing NM_001243
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGTCCTCCTCGCCGCGCTGGGACTGCTGTTCCTGGGGGCGCTACGAGCCTTCCCACAGGATCGAC
  CCTTCGAGGACACCTGTCATGGAAACCCCAGCCACTACTATGACAAGGCTGTCAGGAGGTGCTGTTACCG
  CTGCCCCATGGGGCTGTTCCCGACACAGCAGTGCCCACAGAGGCCTACTGACTGCAGGAAGCAGTGTGAG
  CCTGACTACTACCTGGATGAGGCCGACCGCTGTACAGCCTGCGTGACTTGTTCTCGAGACGACCTCGTGG
  AGAAGACGCCGTGTGCATGGAACTCCTCCCGTGTCTGCGAATGTCGACCCGGCATGTTCTGTTCCACGTC
  TGCCGTCAACTCCTGTGCCCGCTGCTTCTTCCATTCTGTCTGTCCGGCAGGGATGATTGTCAAGTTCCCA
  GGCACGGCGCAGAAGAACACGGTCTGTGAGCCGGCTTCCCCAGGGGTCAGCCCTGCCTGTGCCAGCCCAG
  AGAACTGCAAGGAACCCTCCAGTGGCACCATCCCCCAGGCCAAGCCCACCCCGGTGTCCCCAGCAACCTC
  CAGTGCCAGCACCATGCCTGTAAGAGGGGGCACCCGCCTCGCCCAGGAAGCTGCTTCTAAACTGACGAGG
  GCTCCCGACTCTCCCTCCTCTGTGGGAAGGCCTAGTTCAGATCCAGGTCTGTCCCCAACACAGCCATGCC
  CAGAGGGGTCTGGTGATTGCAGAAAGCAGTGTGAGCCCGACTACTACCTGGACGAGGCCGGCCGCTGCAC
  GGCCTGCGTGAGCTGTTCTCGAGATGACCTTGTGGAGAAGACGCCATGTGCATGGAACTCCTCCCGCACC
  TGCGAATGTCGACCTGGCATGATCTGTGCCACATCAGCCACCAACTCCTGTGCCCGCTGTGTCCCCTACC
  CAATCTGTGCAGCAGAGACGGTCACCAAGCCCCAGGATATGGCTGAGAAGGACACCACCTTTGAGGCGCC
  ACCCCTGGGGACCCAGCCGGACTGCAACCCCACCCCAGAGAATGGCGAGGCGCCTGCCAGCACCAGCCCC
  ACTCAGAGCTTGCTGGTGGACTCCCAGGCCAGTAAGACGCTGCCCATCCCAACCAGCGCTCCCGTCGCTC
  TCTCCTCCACGGGGAAGCCCGTTCTGGATGCAGGGCCAGTGCTCTTCTGGGTGATCCTGGTGTTGGTTGT
  GGTGGTCGGCTCCAGCGCCTTCCTCCTGTGCCACCGGAGGGCCTGCAGGAAGCGAATTCGGCAGAAGCTC
  CACCTGTGCTACCCGGTCCAGACCTCCCAGCCCAAGCTAGAGCTTGTGGATTCCAGACCCAGGAGGAGCT
  CAACGCAGCTGAGGAGTGGTGCGTCGGTGACAGAACCCGTCGCGGAAGAGCGAGGGTTAATGAGCCAGCC
  ACTGATGGAGACCTGCCACAGCGTGGGGGCAGCCTACCTGGAGAGCCTGCCGCTGCAGGATGCCAGCCCG
  GCCGGGGGCCCCTCGTCCCCCAGGGACCTTCCTGAGCCCCGGGTGTCCACGGAGCACACCAATAACAAGA
  TTGAGAAAATCTACATCATGAAGGCTGACACCGTGATCGTGGGGACCGTGAAGGCTGAGCTGCCGGAGGG
  CCGGGGCCTGGCGGGGCCAGCAGAGCCCGAGTTGGAGGAGGAGCTGGAGGCGGACCATACCCCCCACTAC
  CCCGAGCAGGAGACAGAACCGCCTCTGGGCAGCTGCAGCGATGTCATGCTCTCAGTGGAAGAGGAAGGGA
  AAGAAGACCCCTTGCCCACAGCTGCCTCTGGAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219819 representing NM_001243
  Red=Cloning site Green=Tags(s)
  
  MRVLLAALGLLFLGALRAFPQDRPFEDTCHGNPSHYYDKAVRRCCYRCPMGLFPTQQCPQRPTDCRKQCE
  PDYYLDEADRCTACVTCSRDDLVEKTPCAWNSSRVCECRPGMFCSTSAVNSCARCFFHSVCPAGMIVKFP
  GTAQKNTVCEPASPGVSPACASPENCKEPSSGTIPQAKPTPVSPATSSASTMPVRGGTRLAQEAASKLTR
  APDSPSSVGRPSSDPGLSPTQPCPEGSGDCRKQCEPDYYLDEAGRCTACVSCSRDDLVEKTPCAWNSSRT
  CECRPGMICATSATNSCARCVPYPICAAETVTKPQDMAEKDTTFEAPPLGTQPDCNPTPENGEAPASTSP
  TQSLLVDSQASKTLPIPTSAPVALSSTGKPVLDAGPVLFWVILVLVVVVGSSAFLLCHRRACRKRIRQKL
  HLCYPVQTSQPKLELVDSRPRRSSTQLRSGASVTEPVAEERGLMSQPLMETCHSVGAAYLESLPLQDASP
  AGGPSSPRDLPEPRVSTEHTNNKIEKIYIMKADTVIVGTVKAELPEGRGLAGPAEPELEEELEADHTPHY
  PEQETEPPLGSCSDVMLSVEEEGKEDPLPTAASGK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243
• ORF Size: 1785 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243.5
• RefSeq Size: 3686 bp
• RefSeq ORF: 1788 bp
• Locus ID: 943
• UniProt ID: P28908
• Cytogenetics: 1p36.22
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• Gene Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]