AMOTL2 (NM_016201) Human Tagged ORF Clone

CAT#: RG219781

AMOTL2 (tGFP-tagged) - Human angiomotin like 2 (AMOTL2)

Product Images

Circular map for RG219781

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AMOTL2
• Synonyms: LCCP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219781 representing NM_016201
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGACACTGGAAGACTCCTCGGGGACAGTCCTGCACCGCCTCATCCAGGAGCAGCTGCGCTACGGCA
  ACCTGACTGAGACGCGCACGCTGCTAGCCATCCAGCAGCAGGCCCTGAGGGGTGGGGCTGGAACTGGGGG
  TACAGGGAGCCCCCAGGCCTCCCTGGAGATCCTGGCCCCAGAGGACAGTCAGGTGCTGCAGCAGGCCACC
  AGGCAGGAGCCCCAGGGCCAGGAGCACCAGGGCGGTGAGAACCACCTGGCAGAGAACACCCTCTACCGGC
  TATGCCCACAGCCCAGCAAGGGAGAGGAGCTGCCCACCTATGAGGAGGCCAAAGCCCACTCGCAGTACTA
  TGCGGCCCAGCAGGCAGGGACCCGGCCACATGCGGGGGACCGAGATCCCCGTGGGGCCCCGGGAGGCAGT
  CGGAGGCAGGACGAGGCCCTGCGGGAGCTGAGGCATGGGCACGTGCGCTCGTTGAGTGAACGGCTCCTTC
  AGTTGTCCCTGGAGAGGAACGGCGCCCGGGCCCCCAGCCACATGAGCTCCTCCCACAGCTTCCCACAGCT
  GGCCCGCAACCAGCAGGGCCCCCCACTGAGGGGCCCCCCTGCTGAGGGCCCAGAGTCCCGAGGACCCCCA
  CCTCAGTACCCTCATGTTGTACTAGCTCATGAGACCACCACTGCTGTCACTGACCCACGGTACCGTGCCC
  GCGGCAGCCCGCACTTCCAGCATGCTGAAGTCAGGATCCTGCAGGCCCAGGTGCCTCCTGTGTTCCTCCA
  ACAGCAGCAGCAGTACCAGTACCTGCAGCAATCTCAGGAGCACCCCCCTCCCCCACATCCAGCTGCTCTC
  GGCCATGGCCCCCTGAGCTCCCTCAGTCCACCTGCTGTGGAGGGGCCAGTGAGTGCCCAGGCCTCCTCAG
  CCACCTCGGGCAGTGCCCACCTGGCCCAGATGGAGGCCGTGCTGAGGGAGAATGCCAGGCTGCAGAGAGA
  CAATGAGCGGCTGCAGAGGGAGCTGGAGAGCTCTGCGGAGAAGGCTGGCCGCATTGAGAAGCTGGAAAGC
  GAAATCCAGCGGCTCTCTGAGGCCCATGAGAGCCTGACCAGAGCCTCCTCCAAGCGTGAGGCCCTGGAGA
  AGACCATGCGGAACAAGATGGACAGTGAAATGAGGAGGCTGCAAGACTTCAACCGGGATCTTAGAGAGAG
  ATTGGAATCTGCAAATCGCCGCCTGGCAAGCAAGACACAGGAGGCCCAGGCCGGCAGTCAGGACATGGTG
  GCCAAGCTGCTTGCTCAGAGCTACGAACAGCAGCAGGAGCAAGAGAAGCTGGAGCGAGAGATGGCACTGC
  TGCGCGGCGCCATCGAGGACCAGCGGCGGCGTGCCGAGCTGCTGGAGCAGGCTCTGGGCAATGCGCAGGG
  CCGGGCAGCTCGAGCCGAAGAGGAGCTGCGCAAGAAGCAGGCCTATGTGGAGAAAGTGGAGCGGCTGCAG
  CAGGCGCTCGGGCAGCTGCAGGCAGCCTGTGAGAAGCGGGAGCAGCTGGAGCTGCGTCTGCGGACTCGCC
  TGGAGCAGGAACTCAAGGCCCTGCGTGCACAGCAGAGACAGGCAGGTGCCCCAGGTGGTAGCAGTGGCAG
  TGGTGGGTCTCCAGAGCTCAGCGCCCTGCGACTGTCAGAACAACTGCGAGAGAAGGAGGAGCAGATCCTG
  GCGCTGGAGGCCGACATGACCAAGTGGGAGCAGAAGTATTTGGAGGAACGTGCCATGAGGCAGTTTGCCA
  TGGATGCGGCTGCCACGGCTGCTGCTCAGCGTGACACCACTCTCATCCGACATTCCCCCCAGCCCTCACC
  CAGCAGCAGCTTCAATGAGGGTCTGCTCACTGGTGGCCACAGGCATCAGGAGATGGAAAGCAGGTTAAAG
  GTGCTCCATGCCCAGATCCTGGAGAAGGATGCAGTGATCAAGGTCCTTCAGCAGCGCTCCAGGAGAGACC
  CTGGCAAGGCCATCCAGGGCTCCCTGCGGCCTGCCAAGTCGGTGCCATCTGTTTTCGCGGCTGCGGCAGC
  AGGAACCCAGGGCTGGCAAGGGCTCTCTTCTAGTGAGCGACAAACAGCAGACGCCCCTGCTCGGCTGACT
  ACAGCAGACAGAGCACCCACAGAGGAGCCAGTGGTCACAGCTCCCCCTGCTGCCCATGCCAAACACGGGA
  GCAGAGATGGGAGCACCCAGACTGAGGGCCCCCCAGACAGCACCTCCACCTGCCTGCCACCGGAGCCTGA
  CAGCCTTCTGGGGTGCAGCAGTAGCCAGAGAGCAGCCTCTCTGGACTCTGTAGCTACATCCAGAGTCCAG
  GACTTGTCAGACATGGTGGAGATACTGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219781 representing NM_016201
  Red=Cloning site Green=Tags(s)
  
  MRTLEDSSGTVLHRLIQEQLRYGNLTETRTLLAIQQQALRGGAGTGGTGSPQASLEILAPEDSQVLQQAT
  RQEPQGQEHQGGENHLAENTLYRLCPQPSKGEELPTYEEAKAHSQYYAAQQAGTRPHAGDRDPRGAPGGS
  RRQDEALRELRHGHVRSLSERLLQLSLERNGARAPSHMSSSHSFPQLARNQQGPPLRGPPAEGPESRGPP
  PQYPHVVLAHETTTAVTDPRYRARGSPHFQHAEVRILQAQVPPVFLQQQQQYQYLQQSQEHPPPPHPAAL
  GHGPLSSLSPPAVEGPVSAQASSATSGSAHLAQMEAVLRENARLQRDNERLQRELESSAEKAGRIEKLES
  EIQRLSEAHESLTRASSKREALEKTMRNKMDSEMRRLQDFNRDLRERLESANRRLASKTQEAQAGSQDMV
  AKLLAQSYEQQQEQEKLEREMALLRGAIEDQRRRAELLEQALGNAQGRAARAEEELRKKQAYVEKVERLQ
  QALGQLQAACEKREQLELRLRTRLEQELKALRAQQRQAGAPGGSSGSGGSPELSALRLSEQLREKEEQIL
  ALEADMTKWEQKYLEERAMRQFAMDAAATAAAQRDTTLIRHSPQPSPSSSFNEGLLTGGHRHQEMESRLK
  VLHAQILEKDAVIKVLQQRSRRDPGKAIQGSLRPAKSVPSVFAAAAAGTQGWQGLSSSERQTADAPARLT
  TADRAPTEEPVVTAPPAAHAKHGSRDGSTQTEGPPDSTSTCLPPEPDSLLGCSSSQRAASLDSVATSRVQ
  DLSDMVEILI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016201
• ORF Size: 2340 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016201.4
• RefSeq Size: 4984 bp
• RefSeq ORF: 2343 bp
• Locus ID: 51421
• UniProt ID: Q9Y2J4
• Cytogenetics: 3q22.2
• Gene Summary: Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]