Aquaporin 8 (AQP8) (NM_001169) Human Tagged ORF Clone

CAT#: RG219668

AQP8 (tGFP-tagged) - Human aquaporin 8 (AQP8)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AQP8
• Synonyms: AQP-8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219668 representing NM_001169
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGAGAGATAGCCATGTGTGAGCCTGAATTTGGCAATGACAAGGCCAGGGAGCCGAGCGTGGGTG
  GCAGGTGGCGAGTGTCCTGGTACGAACGGTTTGTGCAGCCATGTCTGGTCGAACTGCTGGGCTCTGCTCT
  CTTCATCTTCATCGGGTGCCTGTCGGTCATTGAGAATGGGACGGACACTGGGCTGCTGCAGCCGGCCCTG
  GCCCACGGGCTGGCTTTGGGGCTCGTGATTGCCACGCTGGGGAATATCAGTGGTGGACACTTCAACCCTG
  CGGTGTCCCTGGCAGCCATGCTGATCGGAGGCCTCAACCTGGTGATGCTCCTCCCGTACTGGGTCTCACA
  GCTGCTCGGGGGGATGCTCGGGGCTGCCTTGGCCAAGGCGGTGAGTCCTGAGGAGAGGTTCTGGAATGCA
  TCTGGGGCGGCCTTTGTGACAGTCCAGGAGCAGGGGCAGGTGGCAGGGGCGTTGGTGGCAGAGATCATCC
  TGACGACGCTGCTGGCCCTGGCTGTATGCATGGGTGCCATCAATGAGAAGACAAAGGGCCCTCTGGCCCC
  GTTCTCCATCGGCTTTGCCGTCACCGTGGATATCCTGGCTGGGGGCCCTGTGTCTGGAGGCTGCATGAAT
  CCCGCCCGTGCTTTTGGACCTGCGGTGGTGGCCAACCACTGGAACTTCCACTGGATCTACTGGCTGGGCC
  CACTCCTGGCTGGCCTGCTTGTTGGACTGCTCATTAGGTGCTTCATTGGAGATGGGAAGACCCGCCTCAT
  CCTGAAGGCTCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219668 representing NM_001169
  Red=Cloning site Green=Tags(s)
  
  MSGEIAMCEPEFGNDKAREPSVGGRWRVSWYERFVQPCLVELLGSALFIFIGCLSVIENGTDTGLLQPAL
  AHGLALGLVIATLGNISGGHFNPAVSLAAMLIGGLNLVMLLPYWVSQLLGGMLGAALAKAVSPEERFWNA
  SGAAFVTVQEQGQVAGALVAEIILTTLLALAVCMGAINEKTKGPLAPFSIGFAVTVDILAGGPVSGGCMN
  PARAFGPAVVANHWNFHWIYWLGPLLAGLLVGLLIRCFIGDGKTRLILKAR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001169
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001169.3
• RefSeq Size: 1315 bp
• RefSeq ORF: 786 bp
• Locus ID: 343
• UniProt ID: O94778
• Cytogenetics: 16p12.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. [provided by RefSeq, Jul 2008]