Junctophilin 2 (JPH2) (NM_020433) Human Tagged ORF Clone

CAT#: RG219661

JPH2 (tGFP-tagged) - Human junctophilin 2 (JPH2), transcript variant 1

Product Images

Circular map for RG219661

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Junctophilin 2
• Synonyms: CMH17; JP-2; JP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219661 representing NM_020433
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGGGGCCGCTTCGACTTTGATGATGGAGGGGCGTACTGCGGGGGCTGGGAGGGGGGAAAGGCCC
  ATGGGCATGGACTGTGCACAGGCCCCAAGGGCCAGGGCGAATACTCTGGCTCCTGGAACTTTGGCTTTGA
  GGTGGCAGGTGTCTACACCTGGCCCAGCGGAAACACCTTTGAGGGATACTGGAGCCAGGGCAAACGGCAT
  GGGCTGGGCATAGAGACCAAGGGGCGCTGGCTCTACAAGGGCGAGTGGACACATGGCTTCAAGGGACGCT
  ACGGAATCCGGCAGAGCTCAAGCAGCGGTGCCAAGTATGAGGGCACCTGGAACAATGGCCTGCAAGACGG
  CTATGGCACCGAGACCTATGCTGATGGAGGGACGTACCAAGGCCAGTTCACCAACGGCATGCGCCATGGC
  TACGGAGTACGCCAGAGCGTGCCCTACGGGATGGCCGTGGTGGTGCGCTCGCCGCTGCGCACGTCGCTGT
  CGTCCCTGCGCAGCGAGCACAGCAACGGCACGGTGGCCCCGGACTCTCCCGCCTCGCCGGCCTCCGACGG
  CCCCGCGCTGCCCTCGCCCGCCATCCCGCGTGGCGGCTTCGCGCTCAGCCTCCTGGCCAATGCCGAGGCG
  GCCGCGCGGGCGCCCAAGGGCGGCGGCCTCTTCCAGCGGGGCGCGCTGCTGGGCAAGCTGCGGCGCGCAG
  AGTCGCGCACGTCCGTGGGTAGCCAGCGCAGCCGTGTCAGCTTCCTTAAGAGCGACCTCAGCTCGGGCGC
  CAGCGACGCCGCGTCCACCGCCAGCCTGGGAGAGGCCGCCGAGGGCGCCGACGAGGCCGCACCCTTCGAG
  GCCGATATCGACGCCACCACCACCGAGACCTACATGGGCGAGTGGAAGAACGACAAACGCTCGGGCTTCG
  GCGTGAGCGAACGCTCCAGTGGCCTCCGCTACGAGGGCGAGTGGCTGGACAACCTGCGCCACGGCTATGG
  CTGCACCACGCTGCCCGACGGCCACCGCGAGGAGGGCAAGTACCGCCACAACGTGCTGGTCAAGGACACC
  AAGCGCCGCATGCTGCAGCTCAAGAGCAACAAGGTCCGCCAGAAAGTGGAGCACAGTGTGGAGGGTGCCC
  AGCGCGCCGCTGCTATCGCGCGCCAGAAGGCCGAGATTGCCGCCTCCAGGACAAGCCACGCCAAGGCCAA
  AGCTGAGGCAGCGGAACAGGCCGCCCTGGCTGCCAACCAGGAGTCCAACATTGCTCGCACTTTGGCCAGG
  GAGCTGGCTCCGGACTTCTACCAGCCAGGTCCGGAATATCAGAAGCGCCGGCTGCTGCAGGAGATCCTGG
  AGAACTCGGAGAGCCTGCTGGAGCCCCCCGACCGGGGCGCCGGCGCAGCGGGCCTCCCACAGCCGCCCCG
  CGAGAGCCCGCAGCTGCACGAGCGTGAGACCCCTCGGCCCGAGGGTGGCTCCCCGTCACCGGCCGGGACG
  CCCCCGCAGCCCAAGCGGCCCAGGCCCGGGGTGTCCAAGGACGGCCTGCTGAGCCCAGGCGCCTGGAACG
  GCGAGCCCAGCGGTGAGGGCAGCCGGTCAGTCACTCCGTCCGAGGGCGCGGGCCGCCGCAGCCCCGCGCG
  TCCAGCCACCGAGCGCATGGCCATCGAGGCTCTGCAGGCACCGCCTGCGCCGTCGCGGGAGCCGGAGGTG
  GCGCTTTACCAGGGCTACCACAGCTATGCTGTGCGCACCACGCCGCCCGAGCCCCCACCCTTTGAGGACC
  AGCCCGAGCCCGAGGTCTCCGGGTCCGAGTCCGCGCCCTCGTCCCCGGCCACCGCCCCGCTGCAGGCCCC
  CACGCTCCGAGGCCCCGAGCCTGCACGCGAGACCCCCGCCAAGCTGGAGCCCAAGCCCATCATCCCCAAA
  GCCGAGCCCAGGGCCAAGGCCCGCAAGACTGAGGCTCGAGGGCTGACCAAGGCGGGGGCCAAGAAGAAGG
  CGCGGAAGGAGGCCGCACTGGCGGCAGAGGCGGAGGTGGAGGTGGAAGAGGTCCCCAACACCATCCTCAT
  CTGCATGGTGATCCTGCTGAACATCGGCCTGGCCATCCTCTTTGTTCACCTCCTGACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219661 representing NM_020433
  Red=Cloning site Green=Tags(s)
  
  MSGGRFDFDDGGAYCGGWEGGKAHGHGLCTGPKGQGEYSGSWNFGFEVAGVYTWPSGNTFEGYWSQGKRH
  GLGIETKGRWLYKGEWTHGFKGRYGIRQSSSSGAKYEGTWNNGLQDGYGTETYADGGTYQGQFTNGMRHG
  YGVRQSVPYGMAVVVRSPLRTSLSSLRSEHSNGTVAPDSPASPASDGPALPSPAIPRGGFALSLLANAEA
  AARAPKGGGLFQRGALLGKLRRAESRTSVGSQRSRVSFLKSDLSSGASDAASTASLGEAAEGADEAAPFE
  ADIDATTTETYMGEWKNDKRSGFGVSERSSGLRYEGEWLDNLRHGYGCTTLPDGHREEGKYRHNVLVKDT
  KRRMLQLKSNKVRQKVEHSVEGAQRAAAIARQKAEIAASRTSHAKAKAEAAEQAALAANQESNIARTLAR
  ELAPDFYQPGPEYQKRRLLQEILENSESLLEPPDRGAGAAGLPQPPRESPQLHERETPRPEGGSPSPAGT
  PPQPKRPRPGVSKDGLLSPGAWNGEPSGEGSRSVTPSEGAGRRSPARPATERMAIEALQAPPAPSREPEV
  ALYQGYHSYAVRTTPPEPPPFEDQPEPEVSGSESAPSSPATAPLQAPTLRGPEPARETPAKLEPKPIIPK
  AEPRAKARKTEARGLTKAGAKKKARKEAALAAEAEVEVEEVPNTILICMVILLNIGLAILFVHLLT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020433
• ORF Size: 2088 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020433.5
• RefSeq Size: 4802 bp
• RefSeq ORF: 2091 bp
• Locus ID: 57158
• UniProt ID: Q9BR39
• Cytogenetics: 20q13.12
• Protein Families: Transmembrane
• Gene Summary: Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family. Alternative splicing has been observed at this locus and two variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2008]