RASGRF1 (NM_002891) Human Tagged ORF Clone

CAT#: RG219147

RASGRF1 (tGFP-tagged) - Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1

Product Images

Circular map for RG219147

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RASGRF1
• Synonyms: CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219147 representing NM_002891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAGGGGATCCGGCTGAATGATGGCCACGTCGCGTCCCTGGGACTGCTGGCGCGCAAGGACGGCA
  CGCGCAAAGGCTACCTGAGCAAGCGGAGTTCGGACAACACAAAATGGCAAACCAAGTGGTTCGCGCTGCT
  GCAGAACCTGCTCTTCTACTTCGAGAGCGACTCGAGCTCGCGGCCCTCGGGGCTTTACCTGCTGGAGGGC
  TGCGTCTGCGACCGCGCGCCCTCCCCCAAGCCGGCGCTGTCGGCCAAGGAGCCGCTGGAGAAACAGCATT
  ACTTCACGGTGAACTTCAGCCATGAGAACCAGAAAGCCTTGGAGCTGAGGACAGAGGACGCAAAAGATTG
  TGACGAATGGGTGGCAGCCATTGCACATGCCAGCTACAGGACCCTCGCCACAGAGCATGAGGCATTAATG
  CAGAAATACCTGCACCTGCTGCAGATCGTGGAGACAGAGAAGACCGTGGCCAAGCAGCTTCGGCAGCAGA
  TCGAGGATGGGGAGATCGAGATCGAGCGGCTGAAGGCAGAGATCACATCCCTGCTCAAGGACAATGAGCG
  CATCCAGTCCACCCAGACTGTCGCCCCCAACGATGAAGACAGCGACATCAAGAAAATTAAGAAGGTGCAG
  AGCTTCCTGCGGGGCTGGCTGTGCCGGCGGAAGTGGAAGACCATCATCCAGGACTACATCCGGTCACCCC
  ATGCTGACAGCATGCGCAAGAGGAACCAGGTGGTGTTCAGCATGCTGGAGGCTGAGGCTGAGTACGTGCA
  GCAGCTGCACATCCTTGTCAACAATTTCCTGCGCCCGCTGCGGATGGCCGCCAGCTCCAAGAAGCCTCCC
  ATCACACACGACGACGTCAGCAGCATCTTCCTGAACAGCGAAACCATCATGTTTTTACATCAGATCTTTT
  ACCAAGGCCTGAAGGCCCGCATCTCCAGCTGGCCCACGCTGGTCCTGGCTGACCTATTTGACATCCTGCT
  GCCCATGCTCAACATCTACCAAGAGTTCGTCCGCAACCACCAGTACAGCCTGCAGATCCTGGCCCACTGC
  AAGCAGAACCGTGACTTCGACAAGCTGCTGAAGCACTACGAGGCCAAGCCTGACTGCGAGGAGAGGACGC
  TGGAGACCTTCCTCACCTACCCCATGTTCCAGATCCCCAGGTACATCCTGACCCTCCATGAGCTCCTGGC
  CCACACGCCTCATGAGCACGTTGAGCGCAACAGCCTGGACTACGCCAAGTCCAAACTGGAGGAGCTGTCC
  AGAATAATGCACGATGAAGTAAGTGAGACGGAGAACATCCGGAAAAACCTGGCCATCGAGCGCATGATCA
  TCGAAGGCTGTGAGATCCTCCTGGACACCAGCCAGACCTTTGTGAGACAAGGTTCCCTCATTCAGGTGCC
  CATGTCTGAAAAGGGCAAGATCACCAGGGGGCGCCTGGGGTCTCTCTCCCTAAAGAAAGAGGGCGAGCGA
  CAGTGCTTCCTGTTTTCTAAGCATCTGATTATCTGTACCAGAGGCTCTGGAGGGAAGCTTCACTTGACCA
  AGAATGGAGTCATATCCCTCATTGACTGCACTTTATTGGAGGAGCCAGAAAGCACGGAGGAGGAAGCCAA
  AGGATCCGGCCAAGACATAGATCACTTGGATTTTAAAATCGGGGTGGAGCCAAAGGATTCCCCGCCCTTT
  ACAGTCATCCTAGTGGCCTCGTCCAGACAGGAGAAGGCAGCGTGGACCAGTGACATCAGCCAGTGTGTGG
  ATAACATCCGATGCAATGGGCTCATGATGAACGCATTTGAAGAAAATTCCAAGGTCACTGTGCCGCAGAT
  GATCAAGAGGACCAGGGAGGGGACCAGGGAAGCAGAAATGAGCAGGTCCGACGCCTCCTTATATTGTGAT
  GATGTTGACATTCGCTTCAGCAAAACCATGAACTCCTGCAAAGTGCTGCAGATCCGCTACGCCAGTGTGG
  AGCGGCTGCTGGAGAGGCTGACGGACCTGCGCTTCCTGAGCATCGACTTCCTCAACACCTTCCTGCACTC
  CTACCGCGTCTTCACCACCGCCATCGTGGTCCTGGACAAGCTCATTACCATCTACAAGAAGCCTATCAGT
  GCCATTCCTGCCAGGTGGCTGAGGTCGCTGGAGCTCCTGTTTGCCAGTGGCCAGAACAATAAGCTCCTGT
  ACGGTGAACCCCCCAAGTCCCCGCGCGCCACCCGCAAGTTCTCCTCGCCGCCACCTCTGTCCATCACCAA
  GACATCGTCACCGAGCCGCCGGCGGAAGCTCTCCCTGAACATCCCCATCATCACTGGCGGCAAGGCCCTG
  GACCTGGCCGCCCTCAGCTGCAACTCCAATGGCTACACCAGCATGTACTCGGCCATGTCACCCTTCAGCA
  AGGCCACGCTGGACACCAGCAAGCTCTATGTGTCCAGCAGCTTCACCAACAAGATTCCAGATGAGGGCGA
  TACGACCCCTGAGAAGCCCGAAGACCCTTCAGCGCTCAGCAAGCAGAGCTCAGAAGTCTCCATGAGAGAG
  GAGTCAGATATTGATCAAAACCAGAGTGATGATGGTGATACTGAAACATCACCAACTAAATCTCCAACAA
  CACCCAAATCAGTCAAAAACAAAAATTCTTCAGAGTTCCCACTCTTTTCCTATAACAATGGAGTCGTCAT
  GACCTCCTGTCGTGAACTGGACAATAACCGCAGTGCCTTGTCGGCCGCCTCTGCCTTTGCCATAGCAACC
  GCCGGGGCCAACGAGGGCACCCCAAACAAGGAGAAGTACCGGAGGATGTCCTTAGCCAGTGCAGGGTTTC
  CCCCAGACCAGAGGAATGGAGACAAGGAGTTTGTGATCCGCAGAGCAGCCACCAATCGTGTCTTGAACGT
  GCTCCGCCACTGGGTGTCCAAGCACTCTCAGGACTTTGAGACCAACGATGAGCTCAAATGCAAGGTGATC
  GGCTTCCTGGAAGAAGTCATGCACGACCCGGAGCTCCTGACCCAGGAGCGGAAGGCTGCAGCCAACATCA
  TCAGGACTCTGACCCAGGAGGACCCAGGTGACAACCAGATCACGCTGGAGGAGATCACGCAGATGGCTGA
  AGGCGTGAAGGCTGAGCCCTTTGAAAACCACTCAGCCCTGGAGATCGCGGAGCAGCTGACCCTGCTAGAT
  CACCTCGTCTTCAAGAAGATTCCTTATGAGGAGTTCTTCGGACAAGGATGGATGAAACTGGAAAAGAATG
  AAAGGACCCCTTATATCATGAAAACCACTAAGCACTTCAATGACATCAGTAACTTGATTGCTTCAGAAAT
  CATCCGCAATGAGGACATCAACGCCAGGGTGAGCGCCATCGAGAAGTGGGTGGCCGTAGCTGACATATGC
  CGCTGCCTCCACAACTACAATGCCGTACTGGAGATCACCTCGTCCATGAACCGCAGTGCAATCTTCCGGC
  TCAAAAAGACGTGGCTCAAAGTCTCTAAGCAGACTAAAGCTTTGATTGATAAGCTCCAAAAGCTTGTGTC
  ATCTGAGGGCAGATTTAAGAATCTCAGAGAAGCTCTGAAAAATTGTGACCCACCCTGTGTCCCTTACCTG
  GGGATGTACCTCACCGACCTGGCCTTCATCGAGGAGGGGACGCCCAATTACACGGAAGACGGCCTGGTCA
  ACTTCTCCAAGATGAGGATGATATCCCATATTATCCGAGAGATTCGCCAGTTTCAACAAACTGCCTACAA
  AATAGAGCACCAAGCAAAGGTAACGCAATATTTACTGGACCAATCTTTTGTAATGGATGAAGAAAGCCTC
  TACGAGTCTTCTCTCCGAATAGAACCAAAACTCCCCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219147 representing NM_002891
  Red=Cloning site Green=Tags(s)
  
  MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEG
  CVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALM
  QKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEITSLLKDNERIQSTQTVAPNDEDSDIKKIKKVQ
  SFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPP
  ITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHC
  KQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELS
  RIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGER
  QCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPF
  TVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCD
  DVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPIS
  AIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKAL
  DLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMRE
  ESDIDQNQSDDGDTETSPTKSPTTPKSVKNKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIAT
  AGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVI
  GFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLD
  HLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADIC
  RCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYL
  GMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESL
  YESSLRIEPKLPT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002891
• ORF Size: 3819 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002891.6
• RefSeq Size: 4022 bp
• RefSeq ORF: 3822 bp
• Locus ID: 5923
• UniProt ID: Q13972
• Cytogenetics: 15q25.1
• Domains: IQ, RhoGEF, RasGEFN, PH, RasGEF
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]