CCDC173 (NM_001085447) Human Tagged ORF Clone

CAT#: RG219099

CCDC173 (tGFP-tagged) - Human chromosome 2 open reading frame 77 (C2orf77)

Product Images

Circular map for RG219099

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CCDC173
• Synonyms: C2orf77
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219099 representing NM_001085447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCTCGTCAGAGATGCTGGTACGGTTTGGACGGCGCTGTGGACGGGCGAAGGAAAGTACAGAAA
  TAAGAAACTCTGAAGAAGATCAAGTTCTCTATCTGCCTCTTCTACCTAGCAAAGTAGATCTCCAGCAGGT
  CACCATAATTCCACACGATGAGTGGAAAAGGATTCAAGATAGCCTTGACAGGTTGACAAGAGAAGCAGCA
  TGCCTCCGTGCAGAAAGAAAGGCAAAGAAAGAAATGCATTTGCGATCTCAAGAAGTGGTAAAACACTGGA
  CTAATACATATGCAGGGATGAAAGAACAGAAACTTGAAGCCAAAAAGAAGCGTGATGAAGAAATAGAGGC
  AGAAAGACAAATTCTTGATCTGGAAGAAGAAATATACAAACAAGGAAAAAGAAAAAAGGCCATTGAAAAT
  GCAAAGCAATATCAATTTTACCAGACAGAAAGAGTGAAAAACTTTCATTCGGGACTTCTTCTTAGTAGAG
  TTATGAAAGAACGTGATGCCCAGATTGAATTCCGAAAGAGTAAGATAAAATCAGATAAAAAATGGGAGGA
  ACAGTTGAAACTCAACATTGAAAAAGCTTTTAAAGAAGAACAAGAAAAAGCAGAAAAACGACACAGAGAA
  AGGGTGGCTCTTGCCAAAGATCATCTAAAACAAATAAAGGAACATGAGGAAGAAGAGGAAAGAAGGAAAA
  AATATGAAGAAAAAGATGCTGAGGAAATAAAGCGACAGAATGCATTATATGAAATTGAAATGAGAAAAAA
  ACTAGAAAAGAAAAGAGAAGAGATGCATGAAAGCAGGAGACGGTTTCTTGAACATATGCAGGATAAACAT
  ATTATCAAAGCTGTAGAACAGCAGCAGCAAGAGGAAGAAGATGAAAAGATGAGAAAATTCATCAAAGCAA
  AAAAGCGTCTTATACAAATGGGGAAAGAAAAAGAGGCTGAAACACACAGGCTTATGGAGAAACGAAGAGA
  AAGAATACATAACTTCCTGAGTGAACTGTTGAAAGAGAAACTTGACAATGAAGATATGATTATTGCTAGA
  GATATTGCAGAAGCTGAGGCTGAATGGGAAAAAAGAGAAAGAGAAAAAGATGAAAAAAACAAAGCAGAAT
  TAAAAACAATTGCAGAATATCGAGCCATTGTGATGAAAAATAAAGAGGAAGAAGAAAGACAAAGAAAAAT
  AGAGGCTAAAGAACAATTGCTGGCTGTCATGAAAGCAGATCAGATTTTCTGGGAACATGAAAAGGAGAAA
  AAATGCAAAGCTGATAAAGAACATCAAGAGGTTCAAGATGCCCATATTCAGCAAATGGCCAAAAATAAGT
  TTAATGCAAAGCAAGCAAAACAAGCAGAATTAGATTATTGCAGACTTACTGAAGCTCTTGTGGCTGAAAA
  GGAGAAAGAATTTCAGGACTATGCCAGAGAGGTAATTGAACTTGAATCGGAAACAACAAATAAATATATT
  TACCCTCTTGTAAAAGCTGTACAGGAAGGACCTGGAGGTGGCCGTGGACCAGTGTTTGTGGACAGAGGTG
  GATTAAGACCCAGCTATCAGGCAAATGATGTTACTGGAGTCCAGCTCCCTTTTTATAACTCTCAGGGACC
  AAAATATAATTTTCAAAAGTCAAAGAGAAGGCTAGGTTTTACATGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219099 representing NM_001085447
  Red=Cloning site Green=Tags(s)
  
  MDTSSEMLVRFGRRCGRAKESTEIRNSEEDQVLYLPLLPSKVDLQQVTIIPHDEWKRIQDSLDRLTREAA
  CLRAERKAKKEMHLRSQEVVKHWTNTYAGMKEQKLEAKKKRDEEIEAERQILDLEEEIYKQGKRKKAIEN
  AKQYQFYQTERVKNFHSGLLLSRVMKERDAQIEFRKSKIKSDKKWEEQLKLNIEKAFKEEQEKAEKRHRE
  RVALAKDHLKQIKEHEEEEERRKKYEEKDAEEIKRQNALYEIEMRKKLEKKREEMHESRRRFLEHMQDKH
  IIKAVEQQQQEEEDEKMRKFIKAKKRLIQMGKEKEAETHRLMEKRRERIHNFLSELLKEKLDNEDMIIAR
  DIAEAEAEWEKREREKDEKNKAELKTIAEYRAIVMKNKEEEERQRKIEAKEQLLAVMKADQIFWEHEKEK
  KCKADKEHQEVQDAHIQQMAKNKFNAKQAKQAELDYCRLTEALVAEKEKEFQDYAREVIELESETTNKYI
  YPLVKAVQEGPGGGRGPVFVDRGGLRPSYQANDVTGVQLPFYNSQGPKYNFQKSKRRLGFTW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001085447
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085447.2
• RefSeq Size: 2187 bp
• RefSeq ORF: 1659 bp
• Locus ID: 129881
• UniProt ID: Q0VFZ6
• Cytogenetics: 2q31.1