Integrin alpha 9 (ITGA9) (NM_002207) Human Tagged ORF Clone

CAT#: RG218815

ITGA9 (tGFP-tagged) - Human integrin, alpha 9 (ITGA9)

Product Images

Circular map for RG218815

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Integrin alpha 9
• Synonyms: ALPHA-RLC; ITGA4L; RLC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218815 representing NM_002207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGCCCGGCTGCGCCGAGGGGCGCCGGGAGGCTCCGCGCGCTGCTGCTGGCGCTGGTGGTCGCGG
  GGATCCCCGCGGGCGCCTACAACCTCGACCCGCAGCGCCCCGTGCACTTCCAGGGCCCCGCTGACTCGTT
  CTTCGGCTACGCAGTTCTGGAGCATTTCCACGACAACACGCGCTGGGTCCTTGTGGGCGCACCAAAGGCA
  GATTCCAAATACAGCCCTTCAGTGAAGTCTCCTGGGGCTGTGTTTAAGTGCCGTGTTCACACCAACCCTG
  ACCGGAGATGCACCGAACTGGACATGGCTCGAGGGAAGAATCGGGGCACGTCCTGCGGAAAGACCTGCCG
  GGAAGACCGCGATGATGAGTGGATGGGGGTGAGCCTGGCCCGACAGCCCAAGGCTGATGGCCGTGTGTTG
  GCCTGTGCTCATCGCTGGAAGAACATCTACTATGAAGCCGACCACATCCTACCCCATGGCTTCTGCTACA
  TCATCCCCTCCAACCTCCAGGCCAAAGGCAGGACGCTGATCCCTTGCTATGAAGAGTATAAGAAGAAGTA
  CGGAGAGGAACACGGCTCCTGCCAGGCTGGGATAGCGGGCTTCTTCACCGAGGAGCTGGTGGTGATGGGT
  GCTCCAGGGTCATTTTATTGGGCTGGAACCATCAAAGTGCTGAACCTTACGGACAACACCTATTTAAAAC
  TGAACGACGAAGTGATCATGAACAGGCGGTACACCTACCTGGGCTACGCAGTGACCGCTGGCCACTTCTC
  TCACCCGTCCACCATTGATGTGGTAGGAGGTGCCCCACAGGACAAAGGCATCGGCAAGGTTTATATTTTC
  AGAGCTGACCGAAGATCAGGCACCTTAATTAAGATCTTTCAAGCATCAGGTAAAAAGATGGGCTCTTACT
  TCGGCTCCTCCTTGTGCGCAGTTGACCTGAATGGGGACGGCCTCTCTGACCTGCTGGTGGGGGCCCCCAT
  GTTTTCTGAGATCAGGGATGAGGGACAGGTCACTGTCTACATCAACAGAGGAAATGGAGCCCTCGAGGAG
  CAGCTGGCTCTGACTGGGGATGGTGCCTACAATGCGCACTTTGGAGAGAGCATTGCCAGCCTGGACGATC
  TGGACAATGATGGGTTCCCAGATGTGGCCATTGGTGCACCCAAGGAGGATGACTTCGCAGGGGCGGTCTA
  TATCTATCATGGTGATGCCAGTGGGATAGTCCCTCAGTACTCAATGAAACTGTCTGGGCAGAAGATAAAT
  CCAGTGCTCCGGATGTTTGGTCAGTCCATATCGGGAGGCATTGATATGGATGGAAATGGCTATCCTGATG
  TCACTGTTGGAGCCTTCATGTCCGACAGCGTGGTTCTTCTCAGAGCAAGGCCTGTCATTACGGTGGATGT
  CTCCATCTTCCTCCCGGGCTCCATCAACATCACAGCGCCTCAGTGTCACGACGGACAGCAGCCTGTGAAC
  TGCCTGAACGTCACCACCTGCTTCAGCTTCCATGGCAAACACGTTCCAGGAGAGATTGGCCTGAATTATG
  TTCTGATGGCTGACGTGGCCAAAAAGGAGAAGGGCCAGATGCCCAGGGTCTACTTTGTGCTGCTGGGAGA
  GACCATGGGTCAGGTCACAGAGAAGCTGCAGCTGACTTACATGGAGGAGACGTGTCGTCACTATGTGGCC
  CATGTGAAGCGGAGGGTGCAGGACGTCATCAGCCCGATCGTGTTTGAAGCAGCCTACAGCCTCAGTGAGC
  ATGTGACTGGAGAGGAGGAGAGGGAACTGCCGCCTCTGACACCAGTTCTCCGCTGGAAAAAGGGACAAAA
  GATTGCCCAAAAGAATCAGACTGTTTTTGAAAGGAATTGCCGTTCAGAGGACTGTGCCGCAGACCTGCAG
  CTTCAGGGTAAACTGCTGCTCTCCAGTATGGATGAGAAAACCCTGTATCTAGCTTTGGGGGCTGTGAAGA
  ACATCTCCCTAAACATCTCTATCTCCAACCTCGGAGATGATGCCTATGATGCCAACGTGTCCTTCAATGT
  TTCCCGGGAGCTCTTCTTCATCAACATGTGGCAGAAGGAGGAGATGGGCATCTCCTGTGAGCTGCTGGAA
  TCGGACTTCCTCAAATGCAGCGTGGGATTTCCTTTCATGAGGTCAAAGTCAAAGTATGAATTCAGCGTGA
  TCTTTGATACAAGCCACCTGTCTGGGGAAGAGGAAGTTCTCAGCTTCATTGTTACTGCTCAGAGTGGCAA
  CACGGAGCGCTCTGAATCCCTGCATGACAACACCCTCGTGCTGATGGTGCCACTGATGCACGAGGTGGAC
  ACGTCCATCACCGGAATCATGTCTCCAACCTCCTTTGTATATGGCGAGTCCGTGGACGCAGCCAACTTCA
  TTCAGCTGGATGACCTGGAGTGTCACTTTCAGCCCATCAATATCACCCTTCAGGTCTACAACACTGGCCC
  AAGCACCCTTCCAGGGTCATCTGTCAGCATCTCTTTCCCTAATCGACTCTCATCTGGTGGTGCAGAGATG
  TTTCATGTCCAGGAAATGGTGGTGGGCCAAGAGAAGGGAAACTGCTCTTTCCAGAAAAACCCAACTCCCT
  GCATCATCCCTCAAGAACAAGAAAATATCTTCCACACAATATTTGCTTTTTTCACAAAGTCTGGAAGAAA
  AGTCTTGGACTGTGAAAAACCAGGAATTTCTTGCCTAACAGCACACTGTAACTTTAGTGCTCTTGCTAAA
  GAAGAAAGTCGTACTATAGACATTTACATGCTGCTGAACACAGAAATACTGAAAAAGGACAGTTCGTCTG
  TCATCCAGTTCATGTCCCGCGCCAAGGTGAAGGTGGATCCTGCCCTAAGGGTGGTGGAAATAGCTCATGG
  GAACCCAGAAGAGGTGACGGTGGTCTTCGAGGCCCTGCACAATCTGGAGCCCCGTGGCTACGTCGTGGGG
  TGGATCATCGCCATCAGTTTGTTGGTGGGAATCCTCATCTTCCTGCTGCTGGCCGTGCTGCTCTGGAAGA
  TGGGCTTCTTTCGCCGAAGGTACAAAGAAATTATCGAAGCTGAGAAGAACCGGAAAGAGAATGAAGACAG
  TTGGGACTGGGTCCAGAAAAACCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218815 representing NM_002207
  Red=Cloning site Green=Tags(s)
  
  MGGPAAPRGAGRLRALLLALVVAGIPAGAYNLDPQRPVHFQGPADSFFGYAVLEHFHDNTRWVLVGAPKA
  DSKYSPSVKSPGAVFKCRVHTNPDRRCTELDMARGKNRGTSCGKTCREDRDDEWMGVSLARQPKADGRVL
  ACAHRWKNIYYEADHILPHGFCYIIPSNLQAKGRTLIPCYEEYKKKYGEEHGSCQAGIAGFFTEELVVMG
  APGSFYWAGTIKVLNLTDNTYLKLNDEVIMNRRYTYLGYAVTAGHFSHPSTIDVVGGAPQDKGIGKVYIF
  RADRRSGTLIKIFQASGKKMGSYFGSSLCAVDLNGDGLSDLLVGAPMFSEIRDEGQVTVYINRGNGALEE
  QLALTGDGAYNAHFGESIASLDDLDNDGFPDVAIGAPKEDDFAGAVYIYHGDASGIVPQYSMKLSGQKIN
  PVLRMFGQSISGGIDMDGNGYPDVTVGAFMSDSVVLLRARPVITVDVSIFLPGSINITAPQCHDGQQPVN
  CLNVTTCFSFHGKHVPGEIGLNYVLMADVAKKEKGQMPRVYFVLLGETMGQVTEKLQLTYMEETCRHYVA
  HVKRRVQDVISPIVFEAAYSLSEHVTGEEERELPPLTPVLRWKKGQKIAQKNQTVFERNCRSEDCAADLQ
  LQGKLLLSSMDEKTLYLALGAVKNISLNISISNLGDDAYDANVSFNVSRELFFINMWQKEEMGISCELLE
  SDFLKCSVGFPFMRSKSKYEFSVIFDTSHLSGEEEVLSFIVTAQSGNTERSESLHDNTLVLMVPLMHEVD
  TSITGIMSPTSFVYGESVDAANFIQLDDLECHFQPINITLQVYNTGPSTLPGSSVSISFPNRLSSGGAEM
  FHVQEMVVGQEKGNCSFQKNPTPCIIPQEQENIFHTIFAFFTKSGRKVLDCEKPGISCLTAHCNFSALAK
  EESRTIDIYMLLNTEILKKDSSSVIQFMSRAKVKVDPALRVVEIAHGNPEEVTVVFEALHNLEPRGYVVG
  WIIAISLLVGILIFLLLAVLLWKMGFFRRRYKEIIEAEKNRKENEDSWDWVQKNQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002207
• ORF Size: 3105 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002207.2, NP_002198.2
• RefSeq Size: 3962 bp
• RefSeq ORF: 3108 bp
• Locus ID: 3680
• UniProt ID: Q13797
• Cytogenetics: 3p22.2
• Domains: FG-GAP
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• Gene Summary: This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers. [provided by RefSeq, Jul 2008]