XKR4 (NM_052898) Human Tagged ORF Clone

CAT#: RG218548

XKR4 (tGFP-tagged) - Human XK, Kell blood group complex subunit-related family, member 4 (XKR4)

Product Images

Circular map for RG218548

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: XKR4
• Synonyms: XRG4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218548 representing NM_052898
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTAAATCAGACGGGAGGCTGAAAATGAAGAAAAGCAGCGACGTGGCGTTCACCCCGCTGCAGA
  ACTCGGACCACTCGGGCTCGGTGCAGGGATTGGCTCCAGGCTTGCCGTCGGGGTCGGGAGCCGAGGACGA
  GGAGGCGGCCGGGGGCGGCTGCTGCCCGGACGGCGGCGGCTGCTCGCGCTGCTGCTGCTGCTGCGCCGGG
  AGTGGCGGCTCCGCGGGCTCGGGCGGCTCCGGCGGCGTCGCCGGCCCGGGCGGCGGCGGGGCGGGCTCGG
  CTGCGCTGTGCCTGCGCCTGGGCAGGGAGCAGCGGCGCTACTCACTGTGGGACTGCCTCTGGATCCTGGC
  CGCCGTGGCCGTGTACTTCGCGGACGTGGGCACAGACGTCTGGCTCGCCGTGGACTACTACCTGCGCGGC
  CAGCGCTGGTGGTTCGGGCTCACGCTCTTCTTCGTGGTGCTCGGCTCTCTGTCGGTGCAAGTGTTCAGCT
  TCCGCTGGTTTGTGCACGATTTCAGCACCGAGGACAGCGCCACGGCCGCTGCTGCCTCCAGCTGCCCGCA
  GCCTGGAGCCGATTGCAAGACGGTGGTCGGCGGTGGGTCTGCAGCCGGGGAAGGCGAGGCTCGTCCTTCC
  ACGCCGCAAAGGCAAGCATCTAACGCCAGCAAGAGCAACATCGCCGCGGCCAACAGCGGCAGCAACAGCA
  GCGGGGCTACCCGGGCCAGTGGCAAGCACAGGTCTGCGTCCTGCTCCTTCTGCATCTGGCTCCTGCAGTC
  ACTCATCCACATCTTGCAGCTCGGGCAAATCTGGAGATATTTCCACACAATATACTTAGGTATTCGAAGC
  CGACAGAGTGGGGAGAATGACAGATGGAGGTTTTACTGGAAAATGGTATATGAGTATGCGGATGTGAGTA
  TGCTGCATTTGCTAGCCACCTTTCTGGAAAGTGCTCCACAGCTGGTCCTGCAGCTCTGCATTATCGTACA
  GACTCATAGCTTACAGGCCCTCCAAGGTTTCACAGCGGCAGCTTCCCTCGTGTCCCTGGCCTGGGCCTTG
  GCCTCCTACCAGAAGGCCCTCCGGGACTCTCGAGATGACAAGAAGCCCATCAGCTACATGGCCGTCATCA
  TCCAGTTCTGCTGGCACTTCTTCACCATCGCCGCCAGGGTCATCACGTTTGCCCTCTTTGCCTCGGTTTT
  CCAGCTGTACTTTGGGATCTTCATCGTCCTTCACTGGTGCATCATGACCTTCTGGATCGTCCACTGTGAG
  ACAGAATTCTGTATCACCAAATGGGAAGAGATTGTGTTCGACATGGTGGTGGGGATTATCTATATCTTCA
  GTTGGTTCAATGTCAAGGAAGGCAGGACACGCTGCAGGCTATTCATTTACTATTTTGTGATCCTTTTGGA
  AAATACAGCCTTGAGTGCCCTCTGGTACCTCTACAAGGCTCCCCAGATTGCAGACGCATTTGCCATTCCA
  GCGCTGTGTGTGGTGTTCAGCAGCTTTTTAACTGGCGTTGTTTTTATGCTGATGTATTATGCCTTCTTTC
  ATCCCAATGGACCCAGATTCGGGCAGTCACCAAGTTGTGCTTGTGAGGACCCAGCCGCTGCCTTCACTTT
  GCCCCCAGACGTGGCCACAAGCACCCTACGGTCCATCTCCAACAACCGCAGTGTTGTCAGCGACCGCGAT
  CAGAAATTCGCAGAGCGGGATGGGTGTGTACCTGTCTTTCAAGTGAGGCCCACTGCCCCATCCACCCCAT
  CATCTCGCCCACCACGGATTGAAGAATCAGTCATTAAAATTGACTTGTTCAGGAATAGGTACCCAGCATG
  GGAGAGACATGTTTTGGACCGAAGCCTCCGAAAGGCTATTTTAGCTTTTGAATGTTCCCCATCTCCTCCA
  AGGCTGCAGTACAAAGATGATGCCCTTATTCAGGAGCGGTTGGAGTACGAAACCACTTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218548 representing NM_052898
  Red=Cloning site Green=Tags(s)
  
  MAAKSDGRLKMKKSSDVAFTPLQNSDHSGSVQGLAPGLPSGSGAEDEEAAGGGCCPDGGGCSRCCCCCAG
  SGGSAGSGGSGGVAGPGGGGAGSAALCLRLGREQRRYSLWDCLWILAAVAVYFADVGTDVWLAVDYYLRG
  QRWWFGLTLFFVVLGSLSVQVFSFRWFVHDFSTEDSATAAAASSCPQPGADCKTVVGGGSAAGEGEARPS
  TPQRQASNASKSNIAAANSGSNSSGATRASGKHRSASCSFCIWLLQSLIHILQLGQIWRYFHTIYLGIRS
  RQSGENDRWRFYWKMVYEYADVSMLHLLATFLESAPQLVLQLCIIVQTHSLQALQGFTAAASLVSLAWAL
  ASYQKALRDSRDDKKPISYMAVIIQFCWHFFTIAARVITFALFASVFQLYFGIFIVLHWCIMTFWIVHCE
  TEFCITKWEEIVFDMVVGIIYIFSWFNVKEGRTRCRLFIYYFVILLENTALSALWYLYKAPQIADAFAIP
  ALCVVFSSFLTGVVFMLMYYAFFHPNGPRFGQSPSCACEDPAAAFTLPPDVATSTLRSISNNRSVVSDRD
  QKFAERDGCVPVFQVRPTAPSTPSSRPPRIEESVIKIDLFRNRYPAWERHVLDRSLRKAILAFECSPSPP
  RLQYKDDALIQERLEYETTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052898
• ORF Size: 1950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052898.2
• RefSeq Size: 3925 bp
• RefSeq ORF: 1953 bp
• Locus ID: 114786
• UniProt ID: Q5GH76
• Cytogenetics: 8q12.1
• Protein Families: Transmembrane