PIK3C2B (NM_002646) Human Tagged ORF Clone

CAT#: RG218354

PIK3C2B (tGFP-tagged) - Human phosphoinositide-3-kinase, class 2, beta polypeptide (PIK3C2B)

Product Images

Circular map for RG218354

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIK3C2B
• Synonyms: C2-PI3K
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218354 representing NM_002646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCGACTCAGGGCAATGGGGAACACTGGAAGTCCCTGGAGTCAGTGGGCATCAGCCGCAAAGAGC
  TAGCGATGGCCGAAGCCCTGCAGATGGAGTATGATGCCCTGTCCCGGCTCCGGCATGACAAGGAGGAGAA
  CAGAGCCAAGCAGAACGCAGACCCCTCTCTCATCAGCTGGGATGAGCCTGGGGTAGACTTTTACAGCAAG
  CCAGCAGGAAGGCGGACCGACCTCAAGCTGTTACGCGGTCTCTCTGGCTCTGATCCTACCCTTAACTACA
  ACTCACTCTCCCCACAGGAAGGGCCGCCCAACCACTCTACCTCCCAAGGGCCACAGCCTGGCTCAGATCC
  CTGGCCCAAAGGCTCCCTGTCTGGAGACTATCTCTACATTTTTGATGGTTCAGATGGGGGAGTCTCTTCG
  TCCCCAGGACCAGGGGACATAGAGGGCTCTTGCAAGAAACTATCCCCACCTCCTCTGCCTCCCCGAGCTT
  CTATCTGGGATACCCCTCCCCTGCCTCCCAGAAAGGGGTCCCCCTCATCCTCCAAGATCTCCCAGCCCAG
  TGACATCAACACTTTCTCTTTGGTCGAACAATTGCCGGGCAAACTGCTAGAGCATCGGATCCTAGAAGAG
  GAAGAGGTGCTGGGAGGTGGGGGTCAGGGGCGCCTACTGGGGTCTGTGGACTATGATGGTATCAATGATG
  CAATTACTAGGCTCAACTTGAAATCGACCTATGATGCGGAGATGTTGCGGGATGCCACCAGGGGCTGGAA
  GGAGGGCCGAGGGCCGCTGGACTTCAGCAAAGACACCTCTGGAAAACCCGTGGCCAGGAGCAAGACTATG
  CCCCCTCAGGTGCCCCCCCGCACCTATGCCTCCCGCTATGGCAACCGAAAGAATGCGACGCCTGACAAGA
  ACCGCCGGATTTCTGCAGCCCCGGTGGGCTCCCGGCCCCACACTGTTGCCAATGGCCATGAGTTGTTTGA
  GGTCTCAGAAGAGAGAGATGAGGAGGTTGCTGCATTTTGCCACATGCTGGATATCCTTCGATCTGGCTCT
  GACATCCAAGACTACTTCCTCACTGGCTATGTCTGGAGTGCTGTCACCCCTAGCCCAGAGCACCTCGGGG
  ATGAGGTCAACCTGAAGGTGACTGTGTTGTGTGACAGGCTTCAAGAGGCACTCACTTTCACCTGCAACTG
  TTCCTCCACTGTAGACTTGCTTATCTACCAGACCCTGTGCTACACCCATGATGACCTGAGGAATGTGGAC
  GTGGGTGACTTTGTGCTAAAGCCCTGCGGGCTGGAGGAGTTCCTGCAGAACAAGCATGCCTTGGGCAGTC
  ATGAGTACATCCAATACTGCCGCAAGTTTGACATTGACATTCGGCTACAGCTGATGGAGCAGAAGGTTGT
  GCGCAGTGACCTGGCCCGGACGGTGAATGATGACCAGAGCCCCTCCACCTTGAACTACCTCGTCCATCTC
  CAAGAGAGGCCTGTCAAGCAGACCATCAGCAGGCAGGCCCTGAGTCTTCTGTTCGACACTTACCACAATG
  AGGTGGATGCCTTCCTGCTGGCTGATGGAGACTTCCCACTGAAGGCTGACAGGGTGGTCCAGTCCGTCAA
  GGCCATCTGCAACGCCCTGGCCGCCGTGGAAACCCCTGAGATCACCAGTGCTCTCAACCAGCTGCCCCCC
  TGCCCCTCCCGCATGCAGCCTAAAATTCAGAAGGATCCCAGTGTCTTGGCTGTGAGGGAAAACCGAGAGA
  AGGTCGTGGAAGCCCTGACCGCTGCCATCTTGGACCTGGTGGAGCTGTACTGCAACACATTCAACGCAGA
  CTTCCAGACGGCAGTGCCCGGGAGCCGCAAGCATGACCTGGTCCAGGAGGCCTGCCATTTCGCCAGGTCC
  CTGGCCTTTACTGTCTATGCCACCCACCGCATCCCCATCATCTGGGCTACCAGCTATGAAGATTTCTACC
  TCTCCTGCTCCCTCAGCCATGGCGGCAAGGAGCTGTGCAGCCCCCTGCAGACCCGAAGAGCTCACTTCTC
  CAAGTACCTCTTCCACCTCATCGTCTGGGACCAGCAGATCTGCTTCCCAGTGCAGGTGAACCGGCTGCCT
  CGGGAGACACTGCTGTGTGCCACTCTCTATGCTCTGCCCATCCCCCCACCGGGGAGCTCCTCAGAGGCCA
  ATAAGCAGCGGCGGGTGCCTGAAGCCCTGGGCTGGGTCACTACCCCACTCTTCAACTTCAGGCAGGTCCT
  GACCTGTGGCCGGAAGCTTCTGGGTTTGTGGCCAGCAACACAGGAAAATCCCAGCGCCCGTTGGAGTGCA
  CCTAATTTCCACCAGCCAGACAGTGTCATCCTGCAGATTGACTTCCCCACCTCGGCCTTTGACATCAAGT
  TCACCAGCCCCCCTGGAGACAAGTTCAGCCCCCGCTATGAGTTTGGCAGCCTCCGGGAAGAAGACCAGCG
  CAAGCTTAAAGACATCATGCAGAAAGAGTCCTTGTACTGGCTCACTGATGCTGACAAGAAGCGCCTGTGG
  GAGAAGCGATATTACTGCCACTCGGAGGTGAGCTCGCTCCCCCTGGTGCTCGCCAGCGCCCCCAGCTGGG
  AGTGGGCTTGCCTGCCTGACATCTATGTTCTCCTGAAGCAGTGGACCCACATGAACCACCAGGATGCCCT
  GGGGCTCCTGCATGCCACCTTCCCGGACCAGGAGGTGCGTCGTATGGCTGTGCAGTGGATTGGCTCACTC
  TCAGATGCTGAGCTGCTAGACTACCTGCCCCAGCTGGTACAGGCCCTGAAGTATGAATGCTACCTGGACA
  GCCCGTTGGTGCGCTTCCTCCTGAAACGAGCTGTGTCTGACTTGAGAGTGACTCACTACTTCTTCTGGTT
  ACTGAAGGACGGCCTCAAGGACTCTCAGTTCAGCATCCGCTACCAGTATCTGCTGGCAGCCTTACTGTGC
  TGCTGTGGCAAGGGGCTGAGAGAAGAGTTTAACCGCCAGTGCTGGCTTGTCAATGCCCTGGCCAAACTGG
  CCCAGCAGGTCCGGGAGGCAGCCCCATCTGCAAGGCAGGGAATCCTCCGCACGGGCCTGGAGGAGGTGAA
  GCAGTTCTTTGCCCTCAATGGCTCGTGCCGCTTGCCACTCAGCCCCAGTCTGCTGGTTAAGGGAATTGTG
  CCCAGGGACTGTTCCTACTTCAACTCCAATGCTGTCCCCCTCAAACTCTCCTTCCAAAATGTGGATCCCC
  TGGGTGAGAACATCCGTGTCATCTTCAAGTGTGGGGACGACCTTCGCCAGGACATGCTAACGCTGCAGAT
  GATTCGCATCATGAGCAAGATCTGGGTCCAGGAGGGGCTGGACATGCGCATGGTCATCTTCCGCTGCTTC
  TCCACCGGCCGGGGCAGAGGGATGGTGGAGATGATCCCTAATGCTGAGACCCTGCGTAAGATCCAGGTGG
  AGCATGGGGTGACCGGCTCGTTCAAGGACCGGCCCCTGGCAGACTGGCTGCAGAAACACAACCCTGGGGA
  GGACGAGTATGAGAAGGCTGTGGAGAACTTTATCTACTCCTGCGCTGGCTGCTGCGTGGCCACGTACGTC
  TTGGGCATCTGTGACCGACATAACGACAACATCATGCTGAAGACCACTGGTCACATGTTCCACATTGATT
  TTGGCCGCTTCCTGGGCCATGCCCAGATGTTTGGCAACATCAAGCGGGACCGTGCCCCCTTTGTCTTCAC
  CTCGGACATGGCGTATGTCATCAACGGGGGTGACAAGCCTTCCAGCCGCTTCCATGATTTTGTTGACCTT
  TGCTGCCAAGCCTACAACCTCATTCGCAAGCACACCCACCTCTTCCTCAACCTTCTGGGCCTGATGTTGT
  CCTGTGGGATCCCTGAACTCTCAGACCTGGAGGACCTCAAGTATGTGTACGATGCCCTGAGGCCTCAGGA
  TACAGAGGCCAATGCCACTACCTACTTCACTAGGTTGATTGAGTCCAGCCTGGGCAGTGTAGCCACAAAG
  CTCAATTTTTTCATCCATAATCTGGCTCAGATGAAGTTCACGGGCTCAGATGACCGGCTGACCCTCTCCT
  TTGCCTCCCGAACACACACTCTCAAGAGCTCTGGCCGAATCAGTGATGTTTTCCTCTGCCGCCATGAGAA
  GATCTTCCACCCCAACAAAGGCTATATATATGTGGTAAAGGTGATGCGAGAGAACACTCACGAGGCCACC
  TACATCCAGCGGACCTTTGAGGAGTTCCAGGAATTACACAATAAGTTGCGGCTGCTCTTCCCTTCTTCCC
  ACTTGCCCAGCTTCCCTAGTCGCTTCGTGATCGGCCGCTCCCGGGGAGAGGCGGTGGCCGAGCGGCGGAG
  GGAGGAGCTAAACGGTTACATCTGGCACTTGATCCACGCACCCCCTGAGGTGGCCGAGTGTGATTTGGTG
  TACACCTTCTTCCACCCACTGCCCCGGGATGAGAAGGCTATGGGCACCAGCCCAGCTCCTAAGTCCTCAG
  ATGGCACATGGGCCCGGCCCGTCGGAAAGGTGGGAGGGGAGGTGAAGCTGTCCATCTCCTACAAAAACAA
  TAAACTCTTCATCATGGTGATGCATATTCGGGGCTTGCAACTGCTCCAGGATGGAAATGACCCTGACCCC
  TATGTGAAAATTTACCTCCTTCCTGACCCTCAGAAAACCACTAAGAGGAAAACCAAAGTGGCCCGGAAAA
  CCTGCAATCCTACCTACAATGAGATGTTGGTATATGATGGGATCCCCAAGGGTGACCTGCAGCAGCGGGA
  GCTCCAGCTGAGCGTGCTGAGTGAGCAGGGATTCTGGGAGAACGTCCTCCTCGGTGAGGTGAACATCCGC
  CTGCGAGAGCTGGACCTGGCTCAGGAGAAGACCGGCTGGTTCGCCCTGGGATCTCGAAGTCATGGCACCT
  TG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218354 representing NM_002646
  Red=Cloning site Green=Tags(s)
  
  MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHDKEENRAKQNADPSLISWDEPGVDFYSK
  PAGRRTDLKLLRGLSGSDPTLNYNSLSPQEGPPNHSTSQGPQPGSDPWPKGSLSGDYLYIFDGSDGGVSS
  SPGPGDIEGSCKKLSPPPLPPRASIWDTPPLPPRKGSPSSSKISQPSDINTFSLVEQLPGKLLEHRILEE
  EEVLGGGGQGRLLGSVDYDGINDAITRLNLKSTYDAEMLRDATRGWKEGRGPLDFSKDTSGKPVARSKTM
  PPQVPPRTYASRYGNRKNATPDKNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRSGS
  DIQDYFLTGYVWSAVTPSPEHLGDEVNLKVTVLCDRLQEALTFTCNCSSTVDLLIYQTLCYTHDDLRNVD
  VGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDIDIRLQLMEQKVVRSDLARTVNDDQSPSTLNYLVHL
  QERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPP
  CPSRMQPKIQKDPSVLAVRENREKVVEALTAAILDLVELYCNTFNADFQTAVPGSRKHDLVQEACHFARS
  LAFTVYATHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIVWDQQICFPVQVNRLP
  RETLLCATLYALPIPPPGSSSEANKQRRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENPSARWSA
  PNFHQPDSVILQIDFPTSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDIMQKESLYWLTDADKKRLW
  EKRYYCHSEVSSLPLVLASAPSWEWACLPDIYVLLKQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSL
  SDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAVSDLRVTHYFFWLLKDGLKDSQFSIRYQYLLAALLC
  CCGKGLREEFNRQCWLVNALAKLAQQVREAAPSARQGILRTGLEEVKQFFALNGSCRLPLSPSLLVKGIV
  PRDCSYFNSNAVPLKLSFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCF
  STGRGRGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYV
  LGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDL
  CCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATK
  LNFFIHNLAQMKFTGSDDRLTLSFASRTHTLKSSGRISDVFLCRHEKIFHPNKGYIYVVKVMRENTHEAT
  YIQRTFEEFQELHNKLRLLFPSSHLPSFPSRFVIGRSRGEAVAERRREELNGYIWHLIHAPPEVAECDLV
  YTFFHPLPRDEKAMGTSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQLLQDGNDPDP
  YVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENVLLGEVNIR
  LRELDLAQEKTGWFALGSRSHGTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002646
• ORF Size: 4902 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002646.2, NP_002637.2
• RefSeq Size: 7618 bp
• RefSeq ORF: 4905 bp
• Locus ID: 5287
• UniProt ID: O00750
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq, Jul 2008]