ATAD2 (NM_014109) Human Tagged ORF Clone

CAT#: RG218291

ATAD2 (tGFP-tagged) - Human ATPase family, AAA domain containing 2 (ATAD2)

Product Images

Circular map for RG218291

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATAD2
• Synonyms: ANCCA; CT137; PRO2000
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218291 representing NM_014109
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTTCTCCGCAGCAGCTTGGAGCTGCACAACCACTCCGCGGCCTCGGCCACGGGCTCCTTGGACC
  TGTCCAGTGACTTCCTCAGTCTGGAGCACATCGGCCGGAGGCGGCTCCGCTCGGCCGGCGCGGCGCAGAA
  GAAACCCGCGGCGACCACAGCCAAAGCGGGCGATGGGTCATCAGTTAAGGAAGTTGAAACCTACCACCGG
  ACACGTGCTTTAAGATCTTTGAGAAAAGATGCACAGAATTCTTCAGATTCTAGTTTTGAGAAGAATGTGG
  AAATAACGGAGCAACTTGCTAATGGCAGGCATTTTACAAGGCAGTTGGCCAGACAGCAGGCTGATAAAAA
  AAAAGAAGAGCACAGAGAAGACAAAGTGATTCCAGTTACTCGGTCATTGAGGGCTAGAAACATCGTTCAA
  AGTACAGAACACTTACATGAAGATAATGGTGATGTTGAAGTGCGTCGAAGTTGTAGGATTAGAAGTCGTT
  ATAGTGGTGTAAACCAGTCCATGCTGTTTGACAAACTTATAACTAACACTGCTGAAGCTGTACTTCAAAA
  AATGGATGACATGAAGAAGATGCGTAGACAGCGAATGAGAGAACTTGAAGACTTGGGAGTGTTTAATGAA
  ACAGAAGAAAGCAATCTTAATATGTACACAAGAGGAAAACAGAAAGATATTCAAAGAACTGATGAAGAAA
  CAACTGATAATCAAGAAGGCAGTGTGGAGTCATCTGAAGAGGGTGAAGACCAAGAACATGAAGATGATGG
  TGAAGATGAAGATGATGAAGATGATGATGATGATGACGATGATGATGATGATGATGATGATGAAGATGAT
  GAAGATGAAGAAGATGGAGAAGAAGAGAATCAGAAGCGATATTATCTTAGACAGAGAAAAGCTACTGTTT
  ACTATCAGGCTCCATTGGAAAAACCTCGTCACCAGAGAAAGCCCAACATATTTTATAGTGGCCCAGCTTC
  TCCTGCAAGACCAAGATACCGATTATCTTCCGCAGGACCAAGAAGTCCTTACTGTAAACGAATGAACAGG
  CGAAGGCATGCAATCCACAGTAGTGACTCGACTTCATCTTCCTCCTCTGAAGATGAACAGCACTTTGAGA
  GGCGGAGGAAAAGGAGTCGTAATAGGGCTATCAATAGGTGCCTCCCACTAAATTTTCGGAAAGATGAATT
  AAAAGGCATTTATAAAGATCGAATGAAAATTGGAGCAAGCCTTGCCGATGTTGATCCAATGCAACTAGAT
  TCTTCAGTACGATTTGATAGTGTTGGTGGCCTGTCTAATCATATAGCAGCTCTAAAAGAGATGGTGGTGT
  TTCCATTACTTTATCCAGAAGTCTTTGAAAAATTTAAAATTCAACCCCCAAGAGGTTGTTTGTTTTATGG
  GCCACCTGGAACTGGAAAGACTCTGGTTGCCAGAGCACTTGCCAATGAGTGCAGTCAAGGGGATAAAAGA
  GTAGCATTTTTCATGAGGAAAGGTGCTGATTGTCTAAGTAAATGGGTAGGAGAATCTGAAAGACAGCTAC
  GATTGCTGTTTGATCAGGCCTATCAGATGCGCCCATCAATTATTTTTTTTGACGAAATTGATGGTCTGGC
  TCCAGTACGGTCAAGCAGGCAAGATCAGATTCACAGTTCTATTGTTTCCACCCTGCTAGCTCTTATGGAT
  GGATTGGACAGCAGAGGGGAAATTGTGGTCATTGGTGCTACGAACAGGCTAGATTCTATAGATCCTGCTT
  TACGAAGGCCTGGTCGCTTTGATAGAGAATTCCTCTTTAGCCTGCCTGATAAAGAGGCTCGAAAAGAGAT
  TCTAAAGATTCACACCAGGGATTGGAATCCCAAACCACTGGACACATTTTTAGAAGAGCTAGCAGAAAAC
  TGTGTTGGATACTGTGGAGCAGATATTAAATCAATATGTGCTGAAGCTGCTTTATGTGCTTTACGACGAC
  GCTACCCACAGATCTATACCACTAGTGAGAAACTGCAGTTGGATCTCTCTTCAATTAATATCTCAGCTAA
  GGATTTCGAGGTAGCTATGCAAAAGATGATACCAGCCTCCCAAAGAGCTGTGACATCACCTGGGCAGGCA
  CTGTCCACCGTTGTGAAACCACTCCTGCAAAACACTGTTGACAAGATTTTAGAAGCCCTGCAGAGAGTAT
  TTCCACATGCAGAATTCAGAACAAATAAAACATTAGACTCAGATATTTCTTGTCCTCTGCTAGAAAGTGA
  CTTGGCTTACAGTGATGATGATGTTCCATCAGTTTATGAAAATGGACTTTCTCAGAAATCTTCTCATAAG
  GCAAAAGACAATTTTAATTTTCTTCATTTGAATAGAAATGCTTGTTACCAACCTATGTCTTTTCGACCAA
  GAATATTGATAGTAGGAGAACCAGGATTTGGGCAAGGTTCTCACTTGGCACCAGCTGTCATTCATGCTTT
  GGAAAAGTTTACTGTATATACATTAGACATTCCTGTTCTTTTTGGAGTTAGTACTACATCCCCTGAAGAA
  ACATGTGCCCAGGTGATTCGTGAAGCTAAGAGAACAGCACCAAGTATAGTGTATGTTCCTCATATCCACG
  TGTGGTGGGAAATAGTTGGACCGACACTTAAAGCCACATTTACCACATTATTACAGAATATTCCTTCATT
  TGCTCCAGTTTTACTACTTGCAACTTCTGACAAACCCCATTCCGCTTTGCCAGAAGAGGTGCAAGAATTG
  TTTATCCGTGATTATGGAGAGATTTTTAATGTCCAGTTACCGGATAAAGAAGAACGGACAAAATTTTTTG
  AAGATTTAATTCTAAAACAAGCTGCTAAGCCTCCTATATCAAAAAAGAAAGCAGTTTTGCAGGCTTTGGA
  GGTACTCCCAGTAGCACCACCACCTGAGCCAAGATCACTGACAGCAGAAGAAGTGAAACGACTAGAAGAA
  CAAGAAGAAGATACATTTAGAGAACTGAGGATTTTCTTAAGAAATGTTACACATAGGCTTGCTATTGACA
  AGCGATTCCGAGTGTTTACTAAGCCTGTTGACCCTGATGAGGTTCCTGATTATGTCACTGTAATAAAGCA
  ACCAATGGACCTTTCATCTGTAATCAGTAAAATTGATCTACACAAGTATCTGACTGTGAAAGACTATTTG
  AGAGATATTGATCTAATCTGTAGTAATGCCTTAGAATACAATCCAGATAGAGATCCTGGAGATCGTCTTA
  TTAGGCATAGAGCCTGTGCTTTAAGAGATACTGCCTATGCCATAATTAAAGAAGAACTTGATGAAGACTT
  TGAGCAGCTCTGTGAAGAAATTCAGGAATCTAGAAAGAAAAGAGGTTGTAGCTCCTCCAAATATGCCCCG
  TCTTACTACCATGTGATGCCAAAGCAAAATTCCACTCTTGTTGGTGATAAAAGATCAGACCCAGAGCAGA
  ATGAAAAGCTAAAGACACCGAGTACTCCTGTGGCTTGCAGCACTCCTGCTCAGTTGAAGAGGAAAATTCG
  CAAAAAGTCAAACTGGTACTTAGGCACCATAAAAAAGCGAAGGAAGATTTCACAGGCAAAGGATGATAGC
  CAGAATGCCATAGATCACAAAATTGAGAGTGATACAGAGGAAACTCAAGACACAAGTGTAGATCATAATG
  AGACCGGAAACACAGGAGAGTCTTCGGTGGAAGAAAATGAAAAACAGCAAAATGCCTCTGAAAGCAAACT
  GGAATTGAGAAATAATTCAAATACTTGTAATATAGAGAATGAGCTTGAAGACTCTAGGAAGACTACAGCA
  TGTACAGAATTGAGAGACAAGATTGCTTGTAATGGAGATGCTTCTAGCTCTCAGATAATACATATTTCTG
  ATGAAAATGAAGGAAAAGAAATGTGTGTTCTGCGAATGACTCGAGCTAGACGTTCCCAGGTAGAACAGCA
  GCAGCTCATCACTGTTGAAAAGGCTTTGGCAATTCTTTCTCAGCCTACACCCTCACTTGTTGTGGATCAT
  GAGCGATTAAAAAATCTTTTGAAGACTGTTGTTAAAAAAAGTCAAAACTACAACATATTTCAGTTGGAAA
  ATTTGTATGCAGTAATCAGCCAATGTATTTATCGGCATCGCAAGGACCATGATAAAACATCACTTATTCA
  GAAAATGGAGCAAGAGGTAGAAAACTTCAGTTGTTCCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218291 representing NM_014109
  Red=Cloning site Green=Tags(s)
  
  MVVLRSSLELHNHSAASATGSLDLSSDFLSLEHIGRRRLRSAGAAQKKPAATTAKAGDGSSVKEVETYHR
  TRALRSLRKDAQNSSDSSFEKNVEITEQLANGRHFTRQLARQQADKKKEEHREDKVIPVTRSLRARNIVQ
  STEHLHEDNGDVEVRRSCRIRSRYSGVNQSMLFDKLITNTAEAVLQKMDDMKKMRRQRMRELEDLGVFNE
  TEESNLNMYTRGKQKDIQRTDEETTDNQEGSVESSEEGEDQEHEDDGEDEDDEDDDDDDDDDDDDDDEDD
  EDEEDGEEENQKRYYLRQRKATVYYQAPLEKPRHQRKPNIFYSGPASPARPRYRLSSAGPRSPYCKRMNR
  RRHAIHSSDSTSSSSSEDEQHFERRRKRSRNRAINRCLPLNFRKDELKGIYKDRMKIGASLADVDPMQLD
  SSVRFDSVGGLSNHIAALKEMVVFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKR
  VAFFMRKGADCLSKWVGESERQLRLLFDQAYQMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMD
  GLDSRGEIVVIGATNRLDSIDPALRRPGRFDREFLFSLPDKEARKEILKIHTRDWNPKPLDTFLEELAEN
  CVGYCGADIKSICAEAALCALRRRYPQIYTTSEKLQLDLSSINISAKDFEVAMQKMIPASQRAVTSPGQA
  LSTVVKPLLQNTVDKILEALQRVFPHAEFRTNKTLDSDISCPLLESDLAYSDDDVPSVYENGLSQKSSHK
  AKDNFNFLHLNRNACYQPMSFRPRILIVGEPGFGQGSHLAPAVIHALEKFTVYTLDIPVLFGVSTTSPEE
  TCAQVIREAKRTAPSIVYVPHIHVWWEIVGPTLKATFTTLLQNIPSFAPVLLLATSDKPHSALPEEVQEL
  FIRDYGEIFNVQLPDKEERTKFFEDLILKQAAKPPISKKKAVLQALEVLPVAPPPEPRSLTAEEVKRLEE
  QEEDTFRELRIFLRNVTHRLAIDKRFRVFTKPVDPDEVPDYVTVIKQPMDLSSVISKIDLHKYLTVKDYL
  RDIDLICSNALEYNPDRDPGDRLIRHRACALRDTAYAIIKEELDEDFEQLCEEIQESRKKRGCSSSKYAP
  SYYHVMPKQNSTLVGDKRSDPEQNEKLKTPSTPVACSTPAQLKRKIRKKSNWYLGTIKKRRKISQAKDDS
  QNAIDHKIESDTEETQDTSVDHNETGNTGESSVEENEKQQNASESKLELRNNSNTCNIENELEDSRKTTA
  CTELRDKIACNGDASSSQIIHISDENEGKEMCVLRMTRARRSQVEQQQLITVEKALAILSQPTPSLVVDH
  ERLKNLLKTVVKKSQNYNIFQLENLYAVISQCIYRHRKDHDKTSLIQKMEQEVENFSCSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014109
• ORF Size: 4170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014109.4
• RefSeq Size: 4916 bp
• RefSeq ORF: 4173 bp
• Locus ID: 29028
• UniProt ID: Q6PL18
• Cytogenetics: 8q24.13
• Domains: BROMO, AAA, AAA
• Protein Families: Druggable Genome
• Gene Summary: A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq, Jul 2008]