SCN4A (NM_000334) Human Tagged ORF Clone

CAT#: RG218290

SCN4A (tGFP-tagged) - Human sodium channel, voltage-gated, type IV, alpha subunit (SCN4A)

Product Images

Circular map for RG218290

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SCN4A
• Synonyms: CMS16; HOKPP2; HYKPP; HYPP; Na(V)1.4; NAC1A; Nav1.4; SkM1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218290 representing NM_000334
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGACCATCTCTGTGCACCCTGGCTCGTCTGGGCCCTGAGTGCTTGCGCCCCTTCACCCGGGAGT
  CACTGGCAGCCATAGAACAGCGGGCGGTGGAGGAGGAGGCCCGGCTGCAGCGGAATAAGCAGATGGAGAT
  TGAGGAGCCCGAACGGAAGCCACGAAGTGACTTGGAGGCTGGCAAGAACCTACCCATGATCTACGGAGAC
  CCCCCGCCGGAGGTCATCGGCATCCCCCTGGAGGACCTGGATCCCTACTACAGCAATAAGAAGACCTTCA
  TCGTACTCAACAAGGGCAAGGCCATCTTCCGCTTCTCCGCCACACCTGCTCTCTACCTGCTGAGCCCCTT
  CAGCGTAGTCAGGCGCGGGGCCATCAAGGTGCTCATCCATGCGCTGTTCAGCATGTTCATCATGATCACC
  ATCTTGACCAACTGCGTATTCATGACCATGAGTGACCCGCCTCCCTGGTCCAAGAATGTGGAGTACACCT
  TCACAGGGATCTACACCTTTGAGTCCCTCATCAAGATACTGGCCCGAGGCTTCTGTGTCGACGACTTCAC
  ATTCCTCCGGGACCCCTGGAACTGGCTGGACTTCAGTGTCATCATGATGGCGTACCTGACAGAGTTTGTG
  GACTTGGGCAACATCTCAGCCCTGAGGACCTTCCGGGTGCTGCGGGCCCTCAAAACCATCACGGTCATCC
  CAGGGCTGAAGACGATCGTGGGGGCCCTGATCCAGTCGGTGAAGAAGCTGTCGGATGTGATGATCCTCAC
  TGTCTTCTGCCTGAGCGTCTTTGCGCTGGTAGGACTGCAGCTCTTCATGGGAAACCTGAGGCAGAAGTGT
  GTGCGCTGGCCCCCGCCGTTCAACGACACCAACACCACGTGGTACAGCAATGACACGTGGTACGGCAATG
  ACACATGGTATGGCAATGAGATGTGGTACGGCAATGACTCATGGTATGCCAACGACACGTGGAACAGCCA
  TGCAAGCTGGGCCACCAACGATACCTTTGATTGGGACGCCTACATCAGTGATGAAGGGAACTTCTACTTC
  CTGGAGGGCTCCAACGATGCCCTGCTCTGTGGGAACAGCAGTGATGCTGGGCACTGCCCTAAGGGTTATG
  AGTGCATCAAGACCGGGCGGAACCCCAACTATGGCTACACCAGCTATGACACCTTCAGCTGGGCCTTCTT
  GGCTCTCTTCCGCCTCATGACACAGGACTATTGGGAGAACCTCTTCCAGCTGACCCTTCGAGCAGCTGGC
  AAGACCTACATGATCTTCTTCGTGGTCATCATCTTCCTGGGCTCTTTCTACCTCATCAATCTGATCCTGG
  CCGTGGTGGCCATGGCATATGCCGAGCAGAATGAGGCCACCCTGGCCGAGGATAAGGAGAAAGAGGAGGA
  GTTTCAGCAGATGCTTGAGAAGTTCAAAAAGCACCAGGAGGAGCTGGAGAAGGCCAAGGCCGCCCAAGCT
  CTGGAAGGTGGGGAGGCAGATGGGGACCCAGCCCATGGCAAAGACTGCAATGGCAGCCTGGACACATCGC
  AAGGGGAGAAGGGAGCCCCGAGGCAGAGCGGCAGCGGAGACAGCGGCATCTCCGACGCCATGGAAGAACT
  GGAAGAGGCCCACCAAAAGTGCCCACCATGGTGGTACAAGTGCGCCCACAAAGTGCTCATATGGGACTGC
  TGCGCCCCGTGGCTGAAGTTCAAGAACATCATCCACCTGATCGTCATGGACCCGTTCGTGGACCTGGGCA
  TCACCATCTGCATCGTGCTCAACACCCTCTTCATGGCCATGGAACATTACCCCATGACGGAGCACTTTGA
  CAACGTGCTCACTGTGGGCAACCTGGTCTTCACAGGCATCTTCACAGCAGAGATGGTTCTGAAGCTGATT
  GCCATGGACCCCTACGAGTATTTCCAGCAGGGTTGGAATATCTTCGACAGCATCATCGTCACCCTCAGCC
  TGGTAGAGCTAGGCCTGGCCAACGTACAGGGACTGTCTGTGCTACGCTCCTTCCGTCTGCTGCGGGTCTT
  CAAGCTGGCCAAGTCGTGGCCAACGCTGAACATGCTCATCAAGATCATTGGCAATTCAGTGGGGGCGCTG
  GGTAACCTGACGCTGGTGCTGGCTATCATCGTGTTCATCTTCGCCGTGGTGGGCATGCAGCTGTTTGGCA
  AGAGCTACAAGGAGTGCGTGTGCAAGATTGCCTTGGACTGCAACCTGCCGCGCTGGCACATGCATGATTT
  CTTCCACTCCTTCCTCATCGTCTTCCGCATCCTGTGCGGGGAGTGGATCGAGACCATGTGGGACTGCATG
  GAGGTGGCCGGCCAAGCCATGTGCCTCACCGTCTTCCTCATGGTCATGGTCATCGGCAATCTTGTGGTCC
  TGAACCTGTTCCTGGCTCTGCTGCTGAGCTCCTTCAGCGCCGACAGTCTGGCAGCCTCGGATGAGGATGG
  CGAGATGAACAACCTGCAGATTGCCATCGGGCGCATCAAGTTGGGCATCGGCTTTGCCAAGGCCTTCCTC
  CTGGGGCTGCTGCATGGCAAGATCCTGAGCCCCAAGGACATCATGCTCAGCCTCGGGGAGGCTGACGGGG
  CCGGGGAGGCTGGAGAGGCGGGGGAGACTGCCCCCGAGGATGAGAAGAAGGAGCCGCCCGAGGAGGACCT
  GAAGAAGGACAATCACATCCTGAACCACATGGGCCTGGCTGACGGCCCCCCATCCAGCCTCGAGCTGGAC
  CACCTTAACTTCATCAACAACCCCTACCTGACCATACAGGTGCCCATCGCCTCCGAGGAGTCCGACCTGG
  AGATGCCCACCGAGGAGGAAACCGACACTTTCTCAGAGCCTGAGGATAGCAAGAAGCCGCCGCAGCCTCT
  CTATGATGGGAACTCGTCCGTCTGCAGCACAGCTGACTACAAGCCCCCCGAGGAGGACCCTGAGGAGCAG
  GCAGAGGAGAACCCCGAGGGGGAGCAGCCTGAGGAGTGCTTCACTGAGGCCTGCGTGCAGCGCTGGCCCT
  GCCTCTACGTGGACATCTCCCAGGGCCGTGGGAAGAAGTGGTGGACTCTGCGCAGGGCCTGCTTCAAGAT
  TGTCGAGCACAACTGGTTCGAGACCTTCATTGTCTTCATGATCCTGCTCAGCAGTGGGGCTCTGGCCTTC
  GAGGACATCTACATTGAGCAGCGGCGAGTCATTCGCACCATCCTAGAATATGCCGACAAGGTCTTCACCT
  ACATCTTCATCATGGAGATGCTGCTCAAATGGGTGGCCTACGGCTTTAAGGTGTACTTCACCAACGCCTG
  GTGCTGGCTCGACTTCCTCATCGTGGATGTCTCCATCATCAGCTTGGTGGCCAACTGGCTGGGCTACTCG
  GAGCTGGGACCCATCAAATCCCTGCGGACACTGCGGGCCCTGCGTCCCCTGAGGGCACTGTCCCGATTCG
  AGGGCATGAGGGTGGTGGTGAACGCCCTCCTAGGCGCCATCCCCTCCATCATGAATGTGCTGCTTGTCTG
  CCTCATCTTCTGGCTGATCTTCAGCATCATGGGTGTCAACCTGTTTGCCGGCAAGTTCTACTACTGCATC
  AACACCACCACCTCTGAGAGGTTCGACATCTCCGAGGTCAACAACAAGTCTGAGTGCGAGAGCCTCATGC
  ACACAGGCCAGGTCCGCTGGCTCAATGTCAAGGTCAACTACGACAACGTGGGTCTGGGCTACCTCTCCCT
  CCTGCAGGTGGCCACCTTCAAGGGTTGGATGGACATCATGTATGCAGCCGTGGACTCCCGGGAGAAGGAG
  GAGCAGCCGCAGTACGAGGTGAACCTCTACATGTACCTCTACTTTGTCATCTTCATCATCTTTGGCTCCT
  TCTTCACCCTCAACCTCTTCATTGGCGTCATCATTGACAACTTCAACCAGCAGAAGAAGAAGTTAGGGGG
  GAAAGACATCTTTATGACGGAGGAACAGAAGAAATACTATAACGCCATGAAGAAGCTTGGCTCCAAGAAG
  CCTCAGAAGCCAATTCCCCGGCCCCAGAACAAGATCCAGGGCATGGTGTATGACCTCGTGACGAAGCAGG
  CCTTCGACATCACCATCATGATCCTCATCTGCCTCAACATGGTCACCATGATGGTGGAGACAGACGACCA
  GAGCCAGCTCAAGGTGGACATCCTGTACAACATCAACATGATCTTCATCATCATCTTCACAGGGGAGTGC
  GTGCTCAAGATGCTCGCCCTGCGCCAGTACTACTTCACCGTTGGCTGGAACATCTTTGACTTCGTGGTCG
  TCATCCTGTCCATTGTGGGCCTTGCCCTCTCTGACCTGATCCAGAAGTACTTCGTGTCACCCACGCTGTT
  CCGTGTGATCCGCCTGGCGCGGATTGGGCGTGTCCTGCGGCTGATCCGCGGGGCCAAGGGCATCCGGACG
  CTGCTGTTCGCCCTCATGATGTCGCTGCCTGCCCTCTTCAACATCGGCCTCCTCCTCTTCCTGGTCATGT
  TCATCTACTCCATCTTCGGCATGTCCAACTTTGCCTACGTCAAGAAGGAGTCGGGCATCGATGATATGTT
  CAACTTCGAGACCTTCGGCAACAGCATCATCTGCCTGTTCGAGATCACCACGTCGGCCGGCTGGGACGGG
  CTCCTCAACCCCATCCTCAACAGCGGGCCCCCAGACTGTGACCCCAACCTGGAGAACCCGGGCACCAGTG
  TCAAGGGTGACTGCGGCAACCCCTCCATCGGCATCTGCTTCTTCTGCAGCTATATCATCATCTCCTTCCT
  CATCGTGGTCAACATGTACATCGCCATCATCCTGGAGAACTTCAATGTGGCCACAGAGGAGAGCAGCGAG
  CCCCTTGGTGAAGATGACTTTGAGATGTTCTACGAGACATGGGAGAAGTTCGACCCCGACGCCACCCAGT
  TCATCGCCTACAGCCGCCTCTCAGACTTCGTGGACACCCTGCAGGAACCGCTGAGGATTGCCAAGCCCAA
  CAAGATCAAGCTCATCACACTGGACTTGCCCATGGTGCCAGGGGACAAGATCCACTGCCTGGACATCCTC
  TTTGCCCTGACCAAAGAGGTCCTGGGTGACTCTGGGGAAATGGACGCCCTCAAGCAGACCATGGAGGAGA
  AGTTCATGGCAGCCAACCCCTCCAAGGTGTCCTACGAGCCCATCACCACCACCCTCAAGAGGAAGCACGA
  GGAGGTGTGCGCCATCAAGATCCAGAGGGCCTACCGCCGGCACCTGCTACAGCGCTCCATGAAGCAGGCA
  TCCTACATGTACCGCCACAGCCACGACGGCAGCGGGGATGACGCCCCTGAGAAGGAGGGGCTGCTTGCCA
  ACACCATGAGCAAGATGTATGGCCACGAGAATGGGAACAGCAGCTCGCCAAGCCCGGAGGAGAAGGGCGA
  GGCAGGGGACGCCGGACCCACTATGGGGCTGATGCCCATCAGCCCCTCAGACACTGCCTGGCCTCCCGCC
  CCTCCCCCAGGGCAGACTGTGCGCCCAGGTGTCAAGGAGTCTCTTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218290 representing NM_000334
  Red=Cloning site Green=Tags(s)
  
  MARPSLCTLARLGPECLRPFTRESLAAIEQRAVEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPMIYGD
  PPPEVIGIPLEDLDPYYSNKKTFIVLNKGKAIFRFSATPALYLLSPFSVVRRGAIKVLIHALFSMFIMIT
  ILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCVDDFTFLRDPWNWLDFSVIMMAYLTEFV
  DLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKC
  VRWPPPFNDTNTTWYSNDTWYGNDTWYGNEMWYGNDSWYANDTWNSHASWATNDTFDWDAYISDEGNFYF
  LEGSNDALLCGNSSDAGHCPKGYECIKTGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAG
  KTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDKEKEEEFQQMLEKFKKHQEELEKAKAAQA
  LEGGEADGDPAHGKDCNGSLDTSQGEKGAPRQSGSGDSGISDAMEELEEAHQKCPPWWYKCAHKVLIWDC
  CAPWLKFKNIIHLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLTVGNLVFTGIFTAEMVLKLI
  AMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGAL
  GNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIALDCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCM
  EVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKLGIGFAKAFL
  LGLLHGKILSPKDIMLSLGEADGAGEAGEAGETAPEDEKKEPPEEDLKKDNHILNHMGLADGPPSSLELD
  HLNFINNPYLTIQVPIASEESDLEMPTEEETDTFSEPEDSKKPPQPLYDGNSSVCSTADYKPPEEDPEEQ
  AEENPEGEQPEECFTEACVQRWPCLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAF
  EDIYIEQRRVIRTILEYADKVFTYIFIMEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYS
  ELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCI
  NTTTSERFDISEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKE
  EQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGKDIFMTEEQKKYYNAMKKLGSKK
  PQKPIPRPQNKIQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMIFIIIFTGEC
  VLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRT
  LLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDG
  LLNPILNSGPPDCDPNLENPGTSVKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSE
  PLGEDDFEMFYETWEKFDPDATQFIAYSRLSDFVDTLQEPLRIAKPNKIKLITLDLPMVPGDKIHCLDIL
  FALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSMKQA
  SYMYRHSHDGSGDDAPEKEGLLANTMSKMYGHENGNSSSPSPEEKGEAGDAGPTMGLMPISPSDTAWPPA
  PPPGQTVRPGVKESLV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000334
• ORF Size: 5508 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000334.3, NP_000325.3
• RefSeq Size: 7823 bp
• RefSeq ORF: 5511 bp
• Locus ID: 6329
• UniProt ID: P35499
• Cytogenetics: 17q23.3
• Protein Families: Druggable Genome, Ion Channels: Sodium, Transmembrane
• Gene Summary: Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. It is expressed in skeletal muscle, and mutations in this gene have been linked to several myotonia and periodic paralysis disorders. [provided by RefSeq, Jul 2008]