myosin heavy chain 3 (MYH3) (NM_002470) Human Tagged ORF Clone

CAT#: RG218098

MYH3 (tGFP-tagged) - Human myosin, heavy chain 3, skeletal muscle, embryonic (MYH3)

Product Images

Circular map for RG218098

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: myosin heavy chain 3
• Synonyms: CPSFS1A; CPSFS1B; CPSKF1A; CPSKF1B; DA2A; DA2B; DA2B3; DA8; HEMHC; MYHC-EMB; MYHSE1; SMHCE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218098 representing NM_002470
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAGTGACACTGAAATGGAAGTGTTCGGCATAGCTGCTCCTTTCCTCCGGAAGTCAGAAAAGGAGA
  GGATCGAGGCTCAGAACCAGCCCTTTGATGCCAAGACGTATTGCTTCGTGGTGGACTCAAAGGAAGAATA
  TGCCAAGGGGAAAATCAAGAGTTCTCAGGATGGGAAGGTCACTGTGGAAACTGAGGACAACAGGACCCTG
  GTGGTCAAACCAGAGGATGTGTACGCCATGAACCCCCCCAAGTTCGACAGGATCGAAGACATGGCCATGC
  TGACGCACCTGAATGAGCCAGCCGTGCTGTACAACCTGAAGGACCGTTACACATCTTGGATGATCTATAC
  CTACTCAGGCCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCCGAGGTGGTG
  GAAGGCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAACGCCTATC
  AGTTCATGCTGACTGATCGTGAAAACCAGTCCATTCTGATCACGGGAGAATCCGGGGCAGGAAAGACTGT
  GAACACCAAACGGGTCATCCAGTACTTTGCAACAATTGCAGCTACTGGGGACCTGGCCAAGAAGAAGGAC
  TCCAAAATGAAGGGGACTCTGGAAGATCAAATCATCAGTGCCAATCCCCTGCTGGAGGCCTTTGGGAACG
  CCAAGACTGTGAGGAATGACAACTCCTCCCGTTTTGGCAAGTTCATCCGAATCCATTTTGGAACCACTGG
  GAAGCTGGCCTCTGCAGATATTGAAACTTATCTTCTGGAAAAATCAAGAGTCACTTTCCAGCTGAAGGCT
  GAAAGAAGCTACCACATCTTCTACCAGATTCTTTCTAACAAGAAGCCTGAGCTCATAGAGCTGCTGCTTA
  TTACGACCAACCCTTACGACTACCCGTTCATTAGCCAGGGGGAGATCCTGGTGGCCAGCATAGATGATCG
  AGAGGAGCTGCTGGCTACAGACAGCGCCATTGACATCCTGGGCTTCACCCCAGAAGAGAAATCTGGGCTC
  TACAAGCTGACGGGAGCCGTGATGCACTACGGGAACATGAAGTTCAAGCAGAAGCAGCGAGAGGAGCAGG
  CCGAGCCGGATGGCACAGAAGTGGCTGACAAAACAGCCTATCTGATGGGCCTGAACTCTTCGGACCTCCT
  AAAAGCTTTGTGCTTTCCTAGAGTGAAAGTTGGGAATGAGTACGTTACCAAAGGTCAAACTGTGGATCAG
  GTTCACCATGCTGTGAATGCTCTTTCAAAATCAGTTTATGAAAAGTTGTTCTTGTGGATGGTCACTCGCA
  TTAACCAGCAACTGGATACGAAGCTTCCAAGACAACACTTCATTGGTGTTTTGGACATTGCAGGCTTTGA
  AATCTTTGAGTATAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAGTTTTTC
  AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTCATTGACT
  TCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCTGGAAGA
  GGAGTGCATGTTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCATCTTGGAAAG
  TCCAACAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAGGGCAGAGGCTCACTTCTCACTGATCCACTATG
  CGGGCACCGTGGACTACAGTGTCTCAGGTTGGCTGGAGAAGAACAAGGACCCTCTGAACGAGACTGTGGT
  TGGGCTGTACCAGAAGTCTTCCAACAGGCTCCTGGCACACCTCTATGCCACGTTTGCCACGGCGGATGCT
  GACAGTGGAAAGAAGAAAGTTGCCAAGAAGAAGGGTTCTTCCTTCCAAACTGTCTCTGCCCTTTTCAGGG
  AAAACCTGAACAAGCTGATGTCAAATTTAAGAACTACTCACCCTCATTTTGTGCGTTGTATAATTCCCAA
  TGAAACCAAAACTCCAGGGGCTATGGAACACAGCCTTGTTCTGCACCAGCTGCGGTGTAACGGTGTCCTG
  GAGGGCATCCGCATCTGCAGGAAAGGGTTCCCAAACAGGATTCTCTATGGAGATTTTAAACAAAGATACC
  GAGTGCTGAATGCCAGTGCAATCCTGGAGGGACAATTCATTGACAGCAAGAAAGCCTGTGAAAAGCTTCT
  GGCATCCATTGATATTGACCACACTCAGTACAAATTTGGACATACCAAGGTGTTCTTCAAGGCTGGCTTG
  CTGGGAACCCTGGAAGAGATGCGGGATGACCGCCTGGCCAAACTAATCACCCGGACACAAGCTGTGTGCA
  GAGGGTTCCTCATGCGTGTGGAATTCCAGAAGATGGTGCAGAGGAGGGAGTCCATCTTCTGCATCCAGTA
  CAACATTCGCTCATTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCCCTC
  CTCAAGAGTGCGGAGACTGAGAAAGAGATGGCCACCATGAAGGAAGAATTCCAGAAAACCAAAGATGAAC
  TCGCCAAGTCGGAGGCAAAGAGGAAGGAGCTAGAGGAAAAACTGGTGACTCTGGTCCAAGAGAAAAATGA
  CCTGCAGCTCCAAGTACAAGCTGAAAGCGAAAATTTGTTGGATGCTGAGGAAAGATGCGATCAGCTGATC
  AAAGCCAAATTCCAGCTCGAGGCCAAGATCAAGGAGGTGACAGAGAGAGCTGAAGATGAGGAGGAGATCA
  ATGCTGAGCTGACGGCCAAGAAGAGGAAACTGGAGGATGAATGCTCAGAGCTCAAGAAAGACATTGATGA
  CCTTGAGTTGACCCTGGCCAAGGTTGAGAAGGAGAAGCATGCCACGGAGAACAAGGTTAAAAACCTTACT
  GAGGAACTCTCCGGGTTAGATGAAACAATTGCAAAGTTAACCAGAGAGAAGAAGGCCCTCCAAGAGGCGC
  ACCAGCAGGCCTTGGATGACCTCCAAGCTGAAGAAGACAAAGTCAATTCTTTGAACAAAACCAAGAGCAA
  ACTGGAACAGCAAGTGGAAGACCTGGAAAGCTCCCTAGAACAAGAAAAGAAGCTCCGCGTAGACCTGGAA
  AGGAACAAAAGGAAATTGGAAGGAGACTTGAAGCTTGCTCAAGAGTCCATATTAGATCTGGAGAATGACA
  AGCAACAGCTGGACGAAAGGCTCAAGAAGAAAGATTTTGAATATTGTCAACTTCAAAGCAAAGTGGAAGA
  TGAGCAGACACTGGGCCTCCAGTTTCAGAAGAAAATCAAAGAGTTGCAGGCTCGAATTGAGGAGCTGGAA
  GAGGAGATAGAGGCGGAGAGGGCCACCCGCGCGAAGACAGAGAAACAGCGCAGCGACTATGCCCGGGAGC
  TGGAGGAGCTGAGCGAGCGGCTGGAGGAGGCGGGAGGCGTCACCTCCACGCAGATAGAGCTCAACAAGAA
  GCGGGAGGCTGAGTTCCTGAAGCTGCGCAGGGACCTGGAGGAGGCCACACTGCAGCACGAAGCCATGGTG
  GCCACGCTGAGGAAGAAGCATGCGGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACCTGCAGCGGG
  TCAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATCGATGACCTCTCCAGCAGCATGGA
  GAGTGTGTCGAAATCTAAGGCAAATCTGGAAAAAATCTGCCGAACCCTGGAGGATCAGTTAAGTGAGGCC
  AGGGGCAAGAATGAGGAAATTCAGAGGAGCCTGAGCGAGCTGACCACACAGAAGTCTCGTTTGCAGACCG
  AGGCTGGTGAGCTGAGTCGTCAGCTGGAAGAAAAAGAAAGCATAGTATCCCAACTTTCCAGGAGCAAGCA
  AGCCTTTACCCAGCAAACAGAAGAGCTCAAGAGGCAGCTGGAGGAAGAGAACAAGGCCAAGAACGCCCTG
  GCGCACGCCCTGCAGTCCTCCCGCCACGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAAG
  GCAAAGCTGAGCTGCAGAGGGCGCTGTCCAAGGCCAATAGTGAGGTTGCCCAGTGGAGAACCAAATACGA
  GACGGACGCCATCCAGCGCACAGAAGAGCTGGAGGAGGCCCAAGAAAAACTTGCTCAGCGCCTTCAAGAT
  TCCGAGGAACAGGTTGAGGCAGTGAATGCTAAATGTGCTTCACTGGAGAAGACCAAGCAGAGGCTGCAAG
  GAGAGGTGGAGGATCTGATGGTTGATGTTGAAAGAGCCAATTCCTTGGCCGCCGCTCTGGACAAGAAGCA
  GAGGAACTTTGACAAGGTGTTGGCAGAGTGGAAGACAAAGTGTGAGGAGAGCCAAGCAGAGCTGGAGGCA
  TCCCTGAAGGAGTCCCGCTCCTTGAGCACTGAGCTCTTCAAACTGAAAAATGCCTACGAGGAAGCCTTAG
  ATCAACTTGAAACTGTGAAACGGGAAAATAAGAACTTAGAGCAGGAGATAGCAGATCTCACAGAACAAAT
  TGCTGAAAATGGCAAAACCATCCATGAACTGGAGAAATCAAGAAAGCAGATTGAGCTGGAAAAGGCTGAT
  ATCCAGCTGGCTCTCGAGGAAGCAGAGGCTGCTCTTGAGCATGAAGAAGCCAAGATCCTCCGAATCCAGC
  TTGAATTGACACAAGTGAAATCAGAAATTGATAGAAAGATTGCCGAGAAGGATGAAGAGATCGAGCAGCT
  GAAGAGGAACTACCAGAGAACAGTGGAAACCATGCAGAGCGCCCTGGACGCCGAGGTGCGGAGCAGGAAT
  GAAGCCATCCGGCTCAAGAAGAAGATGGAGGGGGACCTGAATGAAATCGAGATCCAGCTGAGCCACGCCA
  ACCGCCAGGCGGCGGAGACCCTCAAACACCTCAGGAGTGTCCAGGGACAGCTGAAGGATACGCAGCTCCA
  CCTGGATGATGCCCTCCGAGGCCAGGAGGACCTGAAGGAGCAGCTGGCGATTGTGGAGCGCAGAGCCAAC
  CTGCTGCAGGCCGAGGTGGAGGAGCTGCGGGCTACTCTGGAGCAGACGGAGAGGGCCCGGAAACTGGCGG
  AACAGGAGCTCCTGGACTCCAACGAGAGGGTGCAGCTGCTGCATACCCAGAACACCAGCCTCATCCACAC
  CAAGAAGAAGCTGGAGACAGACCTCATGCAGCTCCAGAGTGAGGTAGAAGATGCCAGCAGGGATGCAAGG
  AACGCTGAGGAGAAGGCCAAGAAGGCCATCACGGACGCTGCCATGATGGCGGAGGAGCTGAAGAAGGAGC
  AGGACACCAGCGCCCACCTTGAGCGGATGAAGAAGAACCTGGAACAGACGGTGAAGGACCTGCAGCATCG
  TCTAGATGAGGCCGAGCAGCTGGCGCTGAAGGGCGGGAAGAAGCAGATCCAGAAACTGGAGACCAGGATC
  CGAGAGCTGGAGTTTGAACTTGAGGGAGAGCAGAAGAAGAACACAGAGTCTGTTAAGGGCCTGAGGAAGT
  ATGAGCGGAGGGTCAAGGAGCTGACGTACCAGAGTGAAGAGGACAGGAAGAATGTGCTGAGATTGCAGGA
  TCTGGTGGATAAACTGCAAGTGAAAGTCAAGTCCTACAAGAGGCAGGCGGAGGAGGCTGATGAACAAGCC
  AATGCTCATCTCACCAAATTCCGGAAAGCTCAGCATGAGCTGGAGGAGGCCGAGGAACGTGCGGATATCG
  CAGAATCTCAAGTCAACAAGCTCCGCGCTAAGACTCGAGACTTCACCTCCAGCAGGATGGTGGTCCACGA
  GAGTGAAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218098 representing NM_002470
  Red=Cloning site Green=Tags(s)
  
  MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTL
  VVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVV
  EGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKD
  SKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKA
  ERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDREELLATDSAIDILGFTPEEKSGL
  YKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQ
  VHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFF
  NHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGK
  SNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADA
  DSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVL
  EGIRICRKGFPNRILYGDFKQRYRVLNASAILEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGL
  LGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPL
  LKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLI
  KAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLT
  EELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLE
  RNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELE
  EEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMV
  ATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEA
  RGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNAL
  AHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAQEKLAQRLQD
  SEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEA
  SLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKAD
  IQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRN
  EAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRAN
  LLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDAR
  NAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRI
  RELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQA
  NAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002470
• ORF Size: 5820 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002470.1, NP_002461.1
• RefSeq Size: 6032 bp
• RefSeq ORF: 5823 bp
• Locus ID: 4621
• UniProt ID: P11055
• Cytogenetics: 17p13.1
• Protein Pathways: Tight junction, Viral myocarditis
• Gene Summary: Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome. [provided by RefSeq, Jul 2008]