DNMT1 (NM_001379) Human Tagged ORF Clone

CAT#: RG217851

DNMT1 (GFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 2

Change vector?

  • TrueORF®

USD 2,194


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Size
    • 10 ug

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Specifications

Product Data
Product Name DNMT1 (NM_001379) Human Tagged ORF Clone
Symbol DNMT1
Synonyms ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
Vector pCMV6-AC-GFP
Sequence Data
ORF Nucleotide Sequence
>RG217851 representing NM_001379
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCGCGTACCGCCCCAGCCCGGGTGCCCACACTGGCCGTCCCGGCCATCTCGCTGCCCGACGATG
TCCGCAGGCGGCTCAAAGATTTGGAAAGAGACAGCTTAACAGAAAAGGAATGTGTGAAGGAGAAATTGAA
TCTCTTGCACGAATTTCTGCAAACAGAAATAAAGAATCAGTTATGTGACTTGGAAACCAAATTACGTAAA
GAAGAATTATCCGAGGAGGGCTACCTGGCTAAAGTCAAATCCCTTTTAAATAAAGATTTGTCCTTGGAGA
ACGGTGCTCATGCTTACAACCGGGAAGTGAATGGACGTCTAGAAAACGGGAACCAAGCAAGAAGTGAAGC
CCGTAGAGTGGGAATGGCAGATGCCAACAGCCCCCCCAAACCCCTTTCCAAACCTCGCACGCCCAGGAGG
AGCAAGTCCGATGGAGAGGCTAAGCCTGAACCTTCACCTAGCCCCAGGATTACAAGGAAAAGCACCAGGC
AAACCACCATCACATCTCATTTTGCAAAGGGCCCTGCCAAACGGAAACCTCAGGAAGAGTCTGAAAGAGC
CAAATCGGATGAGTCCATCAAGGAAGAAGACAAAGACCAGGATGAGAAGAGACGTAGAGTTACATCCAGA
GAACGAGTTGCTAGACCGCTTCCTGCAGAAGAACCTGAAAGAGCAAAATCAGGAACGCGCACTGAAAAGG
AAGAAGAAAGAGATGAAAAAGAAGAAAAGAGACTCCGAAGTCAAACCAAAGAACCAACACCCAAACAGAA
ACTGAAGGAGGAGCCGGACAGAGAAGCCAGGGCAGGCGTGCAGGCTGACGAGGACGAAGATGGAGACGAG
AAAGATGAGAAGAAGCACAGAAGTCAACCCAAAGATCTAGCTGCCAAACGGAGGCCCGAAGAAAAAGAAC
CTGAAAAAGTAAATCCACAGATTTCTGATGAAAAAGACGAGGATGAAAAGGAGGAGAAGAGACGCAAAAC
GACCCCCAAAGAACCAACGGAGAAAAAAATGGCTCGCGCCAAAACAGTCATGAACTCCAAGACCCACCCT
CCCAAGTGCATTCAGTGCGGGCAGTACCTGGACGACCCTGACCTCAAATATGGGCAGCACCCACCAGACG
CGGTGGATGAGCCACAGATGCTGACAAATGAGAAGCTGTCCATCTTTGATGCCAACGAGTCTGGCTTTGA
GAGTTATGAGGCGCTTCCCCAGCACAAACTGACCTGCTTCAGTGTGTACTGTAAGCACGGTCACCTGTGT
CCCATCGACACCGGCCTCATCGAGAAGAATATCGAACTCTTCTTTTCTGGTTCAGCAAAACCAATCTATG
ATGATGACCCGTCTCTTGAAGGTGGTGTTAATGGCAAAAATCTTGGCCCCATAAATGAATGGTGGATCAC
TGGCTTTGATGGAGGTGAAAAGGCCCTCATCGGCTTCAGCACCTCATTTGCCGAATACATTCTGATGGAT
CCCAGTCCCGAGTATGCGCCCATATTTGGGCTGATGCAGGAGAAGATCTACATCAGCAAGATTGTGGTGG
AGTTCCTGCAGAGCAATTCCGACTCGACCTATGAGGACCTGATCAACAAGATCGAGACCACGGTTCCTCC
TTCTGGCCTCAACTTGAACCGCTTCACAGAGGACTCCCTCCTGCGACACGCGCAGTTTGTGGTGGAGCAG
GTGGAGAGTTATGACGAGGCCGGGGACAGTGATGAGCAGCCCATCTTCCTGACGCCCTGCATGCGGGACC
TGATCAAGCTGGCTGGGGTCACGCTGGGACAGAGGCGAGCCCAGGCGAGGCGGCAGACCATCAGGCATTC
TACCAGGGAGAAGGACAGGGGACCCACGAAAGCCACCACCACCAAGCTGGTCTACCAGATCTTCGATACT
TTCTTCGCAGAGCAAATTGAAAAGGATGACAGAGAAGACAAGGAGAACGCCTTTAAGCGCCGGCGATGTG
GCGTCTGTGAGGTGTGTCAGCAGCCTGAGTGTGGGAAATGTAAAGCCTGCAAGGACATGGTTAAATTTGG
TGGCAGTGGACGGAGCAAGCAGGCTTGCCAAGAGCGGAGGTGTCCCAATATGGCCATGAAGGAGGCAGAT
GACGATGAGGAAGTCGATGATAACATCCCAGAGATGCCGTCACCCAAAAAAATGCACCAGGGGAAGAAGA
AGAAACAGAACAAGAATCGCATCTCTTGGGTCGGAGAAGCCGTCAAGACTGATGGGAAGAAGAGTTACTA
TAAGAAGGTGTGCATTGATGCGGAAACCCTGGAAGTGGGGGACTGTGTCTCTGTTATTCCAGATGATTCC
TCAAAACCGCTGTATCTAGCAAGGGTCACGGCGCTGTGGGAGGACAGCAGCAACGGGCAGATGTTTCACG
CCCACTGGTTCTGCGCTGGGACAGACACAGTCCTCGGGGCCACGTCGGACCCTCTGGAGCTGTTCTTGGT
GGATGAATGTGAGGACATGCAGCTTTCATATATCCACAGCAAAGTGAAAGTCATCTACAAAGCCCCCTCC
GAAAACTGGGCCATGGAGGGAGGCATGGATCCCGAGTCCCTGCTGGAGGGGGACGACGGGAAGACCTACT
TCTACCAGCTGTGGTATGATCAAGACTACGCGAGATTCGAGTCCCCTCCAAAAACCCAGCCAACAGAGGA
CAACAAGTTCAAATTCTGTGTGAGCTGTGCCCGTCTGGCTGAGATGAGGCAAAAAGAAATCCCCAGGGTC
CTGGAGCAGCTCGAGGACCTGGATAGCCGGGTCCTCTACTACTCAGCCACCAAGAACGGCATCCTGTACC
GAGTTGGTGATGGTGTGTACCTGCCCCCTGAGGCCTTCACGTTCAACATCAAGCTGTCCAGTCCCGTGAA
ACGCCCACGGAAGGAGCCCGTGGATGAGGACCTGTACCCAGAGCACTACCGGAAATACTCCGACTACATC
AAAGGCAGCAACCTGGATGCCCCTGAGCCCTACCGAATTGGCCGGATCAAAGAGATCTTCTGTCCCAAGA
AGAGCAACGGCAGGCCCAATGAGACTGACATCAAAATCCGGGTCAACAAGTTCTACAGGCCTGAGAACAC
CCACAAGTCCACTCCAGCGAGCTACCACGCAGACATCAACCTGCTCTACTGGAGCGACGAGGAGGCCGTG
GTGGACTTCAAGGCTGTGCAGGGCCGCTGCACCGTGGAGTATGGGGAGGACCTGCCCGAGTGCGTCCAGG
TGTACTCCATGGGCGGCCCCAACCGCTTCTACTTCCTCGAGGCCTATAATGCAAAGAGCAAAAGCTTTGA
AGATCCTCCCAACCATGCCCGTAGCCCTGGAAACAAAGGGAAGGGCAAGGGAAAAGGGAAGGGCAAGCCC
AAGTCCCAAGCCTGTGAGCCGAGCGAGCCAGAGATAGAGATCAAGCTGCCCAAGCTGCGGACCCTGGATG
TGTTTTCTGGCTGCGGGGGGTTGTCGGAGGGATTCCACCAAGCAGGCATCTCTGACACGCTGTGGGCCAT
CGAGATGTGGGACCCTGCGGCCCAGGCGTTCCGGCTGAACAACCCCGGCTCCACAGTGTTCACAGAGGAC
TGCAACATCCTGCTGAAGCTGGTCATGGCTGGGGAGACCACCAACTCCCGCGGCCAGCGGCTGCCCCAGA
AGGGAGACGTGGAGATGCTGTGCGGCGGGCCGCCCTGCCAGGGCTTCAGCGGCATGAACCGCTTCAATTC
GCGCACCTACTCCAAGTTCAAAAACTCTCTGGTGGTTTCCTTCCTCAGCTACTGCGACTACTACCGGCCC
CGGTTCTTCCTCCTGGAGAATGTCAGGAACTTTGTCTCCTTCAAGCGCTCCATGGTCCTGAAGCTCACCC
TCCGCTGCCTGGTCCGCATGGGCTATCAGTGCACCTTCGGCGTGCTGCAGGCCGGTCAGTACGGCGTGGC
CCAGACTAGGAGGCGGGCCATCATCCTGGCCGCGGCCCCTGGAGAGAAGCTCCCTCTGTTCCCGGAGCCA
CTGCACGTGTTTGCTCCCCGGGCCTGCCAGCTGAGCGTGGTGGTGGATGACAAGAAGTTTGTGAGCAACA
TAACCAGGTTGAGCTCGGGTCCTTTCCGGACCATCACGGTGCGAGACACGATGTCCGACCTGCCGGAGGT
GCGGAATGGAGCCTCGGCACTGGAGATCTCCTACAACGGGGAGCCTCAGTCCTGGTTCCAGAGGCAGCTC
CGGGGCGCACAGTACCAGCCCATCCTCAGGGACCACATCTGTAAGGACATGAGTGCATTGGTGGCTGCCC
GCATGCGGCACATCCCCTTGGCCCCAGGGTCAGACTGGCGCGATCTGCCCAACATCGAGGTGCGGCTCTC
AGACGGCACCATGGCCAGGAAGCTGCGGTATACCCACCATGACAGGAAGAACGGCCGCAGCAGCTCTGGG
GCCCTCCGTGGGGTCTGCTCCTGCGTGGAAGCCGGCAAAGCCTGCGACCCCGCAGCCAGGCAGTTCAACA
CCCTCATCCCCTGGTGCCTGCCCCACACCGGGAACCGGCACAACCACTGGGCTGGCCTCTATGGAAGGCT
CGAGTGGGACGGCTTCTTCAGCACAACCGTCACCAACCCCGAGCCCATGGGCAAGCAGGGCCGCGTGCTC
CACCCAGAGCAGCACCGTGTGGTGAGCGTGCGGGAGTGTGCCCGCTCCCAGGGCTTCCCTGACACCTACC
GGCTCTTCGGCAACATCCTGGACAAGCACCGGCAGGTGGGCAATGCCGTGCCACCGCCCCTGGCCAAAGC
CATTGGCTTGGAGATCAAGCTTTGTATGTTGGCCAAAGCCCGAGAGAGTGCCTCAGCTAAAATAAAGGAG
GAGGAAGCTGCTAAGGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG217851 representing NM_001379
Red=Cloning site Green=Tags(s)

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRK
EELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRR
SKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSR
ERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDE
KDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHP
PKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLC
PIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMD
PSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQ
VESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDT
FFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEAD
DDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDS
SKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPS
ENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRV
LEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYI
KGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAV
VDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKP
KSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTED
CNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRP
RFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEP
LHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQL
RGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSG
ALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVL
HPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKE
EEAAKD

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag TurboGFP
ACCN NM_001379
ORF Size 4848 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_001379.1, NP_001370
RefSeq Size 5434
RefSeq ORF 4851
Locus ID 1786
Cytogenetics 19p13.2
Protein Families Transcription Factors, Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways
Gene Summary This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016].
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Citations