KCNC4 (NM_001039574) Human Tagged ORF Clone

CAT#: RG217719

KCNC4 (tGFP-tagged) - Human potassium voltage-gated channel, Shaw-related subfamily, member 4 (KCNC4), transcript variant 3

Product Images

Circular map for RG217719

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNC4
• Synonyms: C1orf30; HKSHIIIC; KSHIIIC; KV3.4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217719 representing NM_001039574
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAGCTCGGTGTGTGTCTCCTCCTACCGCGGGCGCAAGTCGGGGAACAAGCCTCCGTCCAAAACAT
  GTCTGAAGGAGGAGATGGCCAAGGGCGAGGCGTCGGAGAAGATCATCATCAACGTGGGCGGCACGCGACA
  TGAGACCTACCGCAGCACCCTGCGCACCCTACCGGGAACCCGCCTCGCCTGGCTGGCCGACCCCGACGGC
  GGGGGCCGGCCCGAGACCGATGGCGGCGGTGTGGGTAGCAGCGGCAGCAGCGGCGGCGGGGGCTGCGAGT
  TCTTCTTCGACAGGCACCCGGGCGTCTTCGCCTACGTGCTCAACTACTACCGCACCGGCAAGCTGCACTG
  CCCCGCGGACGTGTGCGGGCCGCTCTTCGAAGAGGAGCTCACCTTCTGGGGCATCGACGAGACCGACGTG
  GAACCCTGCTGCTGGATGACCTACCGGCAGCACCGCGACGCCGAGGAGGCGCTCGACATCTTCGAGAGCC
  CGGACGGAGGCGGCAGCGGCGCGGGGCCCAGCGACGAGGCCGGCGACGATGAGCGGGAGCTGGCCCTGCA
  GCGACTGGGCCCCCACGAGGGAGGCGCGGGCCATGGCGCCGGGTCTGGGGGCTGCCGCGGCTGGCAGCCC
  CGCATGTGGGCGCTCTTCGAGGATCCCTACTCCTCCCGGGCCGCTAGGGTAGTGGCCTTTGCCTCTCTCT
  TCTTCATCCTGGTCTCCATCACCACTTTCTGCCTGGAGACCCATGAGGCCTTTAATATCGACCGCAACGT
  GACAGAGATCCTCCGCGTAGGGAACATCACCAGCGTGCACTTCCGGCGGGAGGTAGAGACAGAGCCCATC
  CTGACCTACATCGAGGGCGTATGTGTGCTGTGGTTCACACTGGAGTTCCTGGTGCGCATCGTGTGCTGCC
  CCGACACGCTGGACTTCGTCAAGAACCTGCTCAACATCATCGACTTTGTGGCCATCCTGCCCTTCTACCT
  GGAGGTGGGGCTGAGCGGCCTGTCATCCAAGGCGGCCCGCGACGTGCTGGGCTTCCTGCGCGTGGTGCGC
  TTCGTGCGCATCCTGCGTATCTTCAAGCTCACACGCCACTTCGTGGGGCTACGCGTGCTGGGCCACACCC
  TGAGGGCCAGCACCAATGAGTTCCTGCTGCTTATCATCTTCCTGGCCCTGGGTGTGCTCATCTTTGCCAC
  CATGATCTACTACGCTGAGCGCATTGGGGCCAGGCCCTCCGACCCTCGGGGTAATGACCACACCGACTTC
  AAGAACATCCCCATTGGCTTCTGGTGGGCTGTGGTCACCATGACGACACTGGGCTACGGAGACATGTACC
  CCAAGACGTGGTCAGGCATGCTGGTAGGGGCACTGTGTGCACTGGCTGGCGTGCTCACCATCGCCATGCC
  GGTGCCTGTCATCGTCAACAACTTCGGCATGTACTACTCCCTGGCCATGGCCAAGCAGAAGCTGCCCAAG
  AAACGGAAGAAGCACGTGCCACGGCCGGCGCAGCTGGAGTCACCCATGTACTGCAAGTCTGAGGAGACTT
  CCCCCCGGGACAGCACCTGCAGTGATACCAGCCCCCCTGCCCGGGAAGAGGGTATGATCGAGAGGAAACG
  GGCAGACTCTAAGCAGAATGGCGATGCCAACGCAGTGCTGTCTGATGAGGAGGGAGCTGGCCTCACCCAA
  CCCCTGGCCTCCTCCCCGACCCCCGAGGAGCGCCGGGCCCTGCGACGCTCCACCACTCGAGACAGAAACA
  AGAAGGCAGCTGCCTGCTTCCTGCTCAGCACTGGGGACTATGCCTGCGCCGATGGTAGTGTCCGGAAAGA
  GACCTGCCAAGACGCCCTCTCGTCCAACTATGCCCAGGCTGAAGTCCTCACCCTCTCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217719 representing NM_001039574
  Red=Cloning site Green=Tags(s)
  
  MISSVCVSSYRGRKSGNKPPSKTCLKEEMAKGEASEKIIINVGGTRHETYRSTLRTLPGTRLAWLADPDG
  GGRPETDGGGVGSSGSSGGGGCEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFEEELTFWGIDETDV
  EPCCWMTYRQHRDAEEALDIFESPDGGGSGAGPSDEAGDDERELALQRLGPHEGGAGHGAGSGGCRGWQP
  RMWALFEDPYSSRAARVVAFASLFFILVSITTFCLETHEAFNIDRNVTEILRVGNITSVHFRREVETEPI
  LTYIEGVCVLWFTLEFLVRIVCCPDTLDFVKNLLNIIDFVAILPFYLEVGLSGLSSKAARDVLGFLRVVR
  FVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGARPSDPRGNDHTDF
  KNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPK
  KRKKHVPRPAQLESPMYCKSEETSPRDSTCSDTSPPAREEGMIERKRADSKQNGDANAVLSDEEGAGLTQ
  PLASSPTPEERRALRRSTTRDRNKKAAACFLLSTGDYACADGSVRKETCQDALSSNYAQAEVLTLS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039574
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039574.3
• RefSeq Size: 3023 bp
• RefSeq ORF: 1881 bp
• Locus ID: 3749
• UniProt ID: Q03721
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. It generates atypical voltage-dependent transient current that may be important for neuronal excitability. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]