E2F7 (NM_203394) Human Tagged ORF Clone

CAT#: RG217596

E2F7 (tGFP-tagged) - Human E2F transcription factor 7 (E2F7)

Product Images

Circular map for RG217596

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: E2F7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217596 representing NM_203394
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTAAATTGTTTAACACTAAAAGACCTGATCAGCCCCAGGCAGCCCAGACTAGATTTTGCAGTTG
  AAGATGGGGAAAATGCACAAAAGGAAAATATATTTGTTGATCGATCAAGGATGGCCCCGAAGACTCCAAT
  AAAAAATGAACCAATTGATTTATCGAAGCAAAAAAAATTTACTCCAGAAAGAAATCCCATTACTCCAGTT
  AAGCTTGTTGACAGACAGCAAGCGGAACCATGGACACCCACAGCTAACCTGAAGATGCTCATTAGTGCTG
  CCAGCCCAGATATAAGGGACCGGGAGAAGAAAAAGGGACTATTCCGACCCATTGAAAACAAGGACGATGC
  ATTTACAGATTCTCTACAGCTTGATGTTGTTGGGGACAGTGCTGTGGACGAATTTGAAAAGCAAAGGCCA
  AGCAGAAAACAGAAAAGTTTAGGACTCCTGTGCCAGAAGTTTCTAGCTCGCTATCCAAGTTATCCCTTGT
  CAACTGAGAAAACTACCATCTCCCTAGATGAAGTTGCTGTCAGTCTTGGTGTGGAAAGGAGACGCATCTA
  TGACATTGTAAATGTGCTGGAGTCGCTGCATCTGGTCAGCCGGGTGGCTAAGAATCAGTATGGCTGGCAT
  GGACGGCACAGCCTGCCAAAAACCCTGAGGAACCTCCAGAGACTAGGAGAGGAGCAGAAATATGAAGAGC
  AAATGGCCTACCTCCAACAGAAAGAGCTGGACCTGATAGATTATAAATTTGGAGAACGTAAAAAAGATGG
  TGATCCAGATTCCCAGGAACAACAGTTACTGGATTTCTCTGAACCCGACTGTCCCTCTTCATCTGCAAAC
  AGTAGAAAAGACAAGTCTCTGAGAATTATGAGCCAGAAGTTTGTCATGCTGTTCCTCGTCTCCAAAACCA
  AGATTGTCACTCTGGATGTGGCTGCCAAAATACTGATAGAAGAAAGCCAAGATGCCCCGGACCATAGTAA
  ATTTAAAACAAAGGTACGACGCCTCTATGACATAGCCAATGTTCTGACCAGCTTGGCTCTGATAAAGAAA
  GTGCATGTAACAGAAGAGCGAGGTCGTAAACCAGCCTTCAAGTGGATCGGGCCTGTGGACTTCAGCTCAA
  GTGATGAAGAACTGGTGGATGTTTCTGCATCTGTCTTACCAGAATTGAAAAGAGAAACATATGGCCAGAT
  TCAAGTCTGTGCAAAACAGAAGCTGGCTCGCCATGGTTCTTTTAACACAGTTCAGGCTTCTGAGAGGATC
  CAGAGGAAAGTGAACTCAGAACCGAGCAGCCCGTACAGAGAAGAACAAGGATCAGGTGGCTACTCTTTAG
  AAATTGGAAGCCTGGCAGCTGTCTATAGACAGAAAATAGAAGACAATTCACAGGGAAAAGCCTTTGCCAG
  TAAGAGAGTGGTGCCTCCATCAAGCAGCTTGGACCCTGTTGCTCCTTTCCCTGTCCTCTCTGTTGACCCA
  GAATATTGTGTTAATCCTTTAGCCCACCCAGTATTTTCTGTTGCTCAGACGGACCTGCAGGCATTCTCCA
  TGCAGAACGGTCTGAATGGACAAGTGGATGTCTCACTTGCTTCTGCAGCCTCTGCTGTGGAGAGCCTGAA
  GCCAGCACTCCTTGCTGGCCAGCCTCTAGTGTATGTGCCCTCTGCCTCACTGTTCATGCTGTATGGAAGT
  CTGCAGGAGGGACCAGCGTCAGGGTCAGGGTCAGAGAGGGATGACAGAAGCTCAGAAGCCCCAGCCACAG
  TAGAGCTGTCATCTGCACCCTCAGCTCAGAAGCGCCTCTGTGAGGAGAGGAAACCTCAGGAGGAGGATGA
  GCCAGCCACTAAAAGGCAAAGTAGGGAATATGAAGACGGCCCGCTGTCGCTTGTCATGCCCAAGAAACCC
  TCAGATTCCACAGACCTTGCCTCTCCCAAGACTATGGGTAACAGGGCATCTATACCCCTCAAAGACATTC
  ATGTGAATGGCCAACTCCCTGCTGCAGAAGAGATTTCAGGAAAGGCAACAGCAAACTCTCTTGTTTCTTC
  TGAGTGGGGAAATCCTTCAAGAAATACAGATGTTGAAAAGCCTTCAAAAGAAAATGAAAGCACCAAAGAG
  CCTTCTTTGCTACAATATCTTTGTGTGCAGTCTCCTGCAGGATTAAATGGTTTCAATGTACTTTTATCTG
  GCAGTCAAACCCCCCCTACTGTGGGCCCGTCCTCAGGTCAGCTGCCGTCTTTCAGTGTCCCTTGCATGGT
  CTTACCATCTCCACCTCTGGGCCCTTTTCCTGTTCTCTATTCTCCTGCAATGCCGGGCCCGGTTTCTTCT
  ACTCTTGGTGCTCTCCCAAACACAGGACCTGTGAATTTCAGCTTGCCTGGCCTTGGATCAATAGCCCAGC
  TTCTCGTCGGCCCCACAGCTGTGGTTAATCCAAAGTCGTCCACACTCCCTTCTGCAGACCCTCAGCTTCA
  GAGTCAGCCCTCACTAAACCTAAGTCCAGTGATGTCAAGGTCACACAGTGTCGTCCAACAACCTGAGTCC
  CCCGTTTACGTGGGACATCCAGTCTCAGTAGTAAAATTACATCAGTCACCAGTTCCAGTGACCCCCAAGA
  GCATCCAACGCACACATCGTGAGACGTTTTTCAAGACACCCGGCAGCCTTGGAGACCCTGTCCTGAAGAG
  AAGAGAAAGGAACCAGTCACGAAACACCAGCTCGGCCCAGAGGAGACTAGAAATCCCCAGCGGCGGCGCT
  GAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217596 representing NM_203394
  Red=Cloning site Green=Tags(s)
  
  MEVNCLTLKDLISPRQPRLDFAVEDGENAQKENIFVDRSRMAPKTPIKNEPIDLSKQKKFTPERNPITPV
  KLVDRQQAEPWTPTANLKMLISAASPDIRDREKKKGLFRPIENKDDAFTDSLQLDVVGDSAVDEFEKQRP
  SRKQKSLGLLCQKFLARYPSYPLSTEKTTISLDEVAVSLGVERRRIYDIVNVLESLHLVSRVAKNQYGWH
  GRHSLPKTLRNLQRLGEEQKYEEQMAYLQQKELDLIDYKFGERKKDGDPDSQEQQLLDFSEPDCPSSSAN
  SRKDKSLRIMSQKFVMLFLVSKTKIVTLDVAAKILIEESQDAPDHSKFKTKVRRLYDIANVLTSLALIKK
  VHVTEERGRKPAFKWIGPVDFSSSDEELVDVSASVLPELKRETYGQIQVCAKQKLARHGSFNTVQASERI
  QRKVNSEPSSPYREEQGSGGYSLEIGSLAAVYRQKIEDNSQGKAFASKRVVPPSSSLDPVAPFPVLSVDP
  EYCVNPLAHPVFSVAQTDLQAFSMQNGLNGQVDVSLASAASAVESLKPALLAGQPLVYVPSASLFMLYGS
  LQEGPASGSGSERDDRSSEAPATVELSSAPSAQKRLCEERKPQEEDEPATKRQSREYEDGPLSLVMPKKP
  SDSTDLASPKTMGNRASIPLKDIHVNGQLPAAEEISGKATANSLVSSEWGNPSRNTDVEKPSKENESTKE
  PSLLQYLCVQSPAGLNGFNVLLSGSQTPPTVGPSSGQLPSFSVPCMVLPSPPLGPFPVLYSPAMPGPVSS
  TLGALPNTGPVNFSLPGLGSIAQLLVGPTAVVNPKSSTLPSADPQLQSQPSLNLSPVMSRSHSVVQQPES
  PVYVGHPVSVVKLHQSPVPVTPKSIQRTHRETFFKTPGSLGDPVLKRRERNQSRNTSSAQRRLEIPSGGA
  D
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203394
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203394.1, NP_976328.1
• RefSeq Size: 5728 bp
• RefSeq ORF: 2736 bp
• Locus ID: 144455
• UniProt ID: Q96AV8
• Cytogenetics: 12q21.2
• Protein Families: Transcription Factors
• Gene Summary: E2F transcription factors, such as E2F7, play an essential role in the regulation of cell cycle progression (Di Stefano et al., 2003 [PubMed 14633988]).[supplied by OMIM, May 2008]