FEZ2 (NM_001042548) Human Tagged ORF Clone

CAT#: RG217477

FEZ2 (tGFP-tagged) - Human fasciculation and elongation protein zeta 2 (zygin II) (FEZ2), transcript variant 2

Product Images

Circular map for RG217477

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FEZ2
• Synonyms: HUM3CL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217477 representing NM_001042548
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGACGGGGACTGGCAGGATTTCTATGAGTTCCAGGAGCCGGCCCGGAGCCTCCTGGACCAGG
  AGAACTGTAACGCGAGCCCCGAGCCTGGGGCGGAGGCGGGGGCCGAGGCGGGTGGGGGCGCCGACGGTTT
  CCCGGCCCCGGCCTGCAGCTTGGAGGAGAAGCTGAGCCTGTGCTTCCGCCCCTCGGATCCGGGCGCCGAG
  CCCCCGAGGACGGCCGTGCGGCCCATCACGGAGCGCAGCCTCCTGCAGGGGGACGAGATTTGGAATGCCC
  TGACAGATAATTATGGGAATGTGATGCCTGTAGACTGGAAGTCATCGCATACTAGGACCTTGCACTTGCT
  TACTCTGAACCTCTCAGAAAAAGGGGTAAGTGACAGTTTGCTCTTTGATACATCAGATGATGAAGAGCTG
  AGAGAACAGCTGGATATGCACTCAATCATCGTCTCCTGTGTTAATGATGAACCCCTCTTCACGGCAGACC
  AGGTTATTGAAGAAATTGAAGAAATGATGCAGGAATCACCGGACCCAGAAGATGATGAAACCCCTACACA
  GTCAGATCGGCTTTCAATGCTTTCCCAGGAAATTCAAACTCTCAAGAGGTCTAGTACCGGCAGTTATGAA
  GAGAGAGTGAAAAGGCTCTCAGTGTCTGAGTTAAATGAAATCCTGGAAGAAATTGAGACTGCCATTAAGG
  AGTACTCTGAGGAGCTGGTGCAGCAGTTGGCTTTACGAGATGAACTGGAGTTTGAAAAGGAAGTGAAAAA
  CAGCTTTATTTCTGTTCTTATTGAAGTGCAAAACAAACAGAAAGAGCACAAAGAAACAGCAAAAAAGAAA
  AAGAAACTAAAAAATGGCAGCTCTCAGAATGGGAAGAATGAGAGAAGTCATATGCCCGGCACACGCTTCA
  GCATGGAAGGGATCTCAAATGTCATTCAGAATGGCCTCCGCCACACCTTTGGAAATTCAGGTGGAGAGAA
  ACAGTATTTGACTACAGTCATTCCTTATGAGAAAAAAAACGGACCACCGTCTGTTGAAGATCTTCAAATA
  TTAACAAAAATTCTTCGTGCCATGAAGGAGGACAGTGAAAAAGTTCCGAGCTTGTTAACTGATTATATTC
  TGAAAGTTCTGTGTCCTACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217477 representing NM_001042548
  Red=Cloning site Green=Tags(s)
  
  MAADGDWQDFYEFQEPARSLLDQENCNASPEPGAEAGAEAGGGADGFPAPACSLEEKLSLCFRPSDPGAE
  PPRTAVRPITERSLLQGDEIWNALTDNYGNVMPVDWKSSHTRTLHLLTLNLSEKGVSDSLLFDTSDDEEL
  REQLDMHSIIVSCVNDEPLFTADQVIEEIEEMMQESPDPEDDETPTQSDRLSMLSQEIQTLKRSSTGSYE
  ERVKRLSVSELNEILEEIETAIKEYSEELVQQLALRDELEFEKEVKNSFISVLIEVQNKQKEHKETAKKK
  KKLKNGSSQNGKNERSHMPGTRFSMEGISNVIQNGLRHTFGNSGGEKQYLTTVIPYEKKNGPPSVEDLQI
  LTKILRAMKEDSEKVPSLLTDYILKVLCPT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042548
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042548.2
• RefSeq Size: 2110 bp
• RefSeq ORF: 1143 bp
• Locus ID: 9637
• UniProt ID: Q9UHY8
• Cytogenetics: 2p22.2
• Gene Summary: This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin. [provided by RefSeq, Jul 2008]