EGFR (NM_201282) Human Tagged ORF Clone
EGFR (GFP-tagged) - Human epidermal growth factor receptor (EGFR), transcript variant 2
|Product Name||EGFR (NM_201282) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ERBB; ERBB1; ERRP; HER1; mENA; NISBD2; PIG61|
|E. coli Selection||Ampicillin (100 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1884 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2239 bp|
|RefSeq ORF||1887 bp|
|Protein Families||Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane|
|Protein Pathways||Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton|
|Gene Summary||The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020]|
|cDNA Clone Resources|
|RC600002||EGFR (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens epidermal growth factor receptor, transcript variant 2, Signal peptide (1-24) plus EC domain (25-628)||
|SC308009||EGFR (untagged)-Human epidermal growth factor receptor (EGFR), transcript variant 2||
|RC217223L3||Lenti-ORF clone of EGFR (Myc-DDK-tagged)-Human epidermal growth factor receptor (EGFR), transcript variant 2||
|RC217223L4||Lenti-ORF clone of EGFR (mGFP-tagged)-Human epidermal growth factor receptor (EGFR), transcript variant 2||
|RC217223||EGFR (Myc-DDK-tagged)-Human epidermal growth factor receptor (EGFR), transcript variant 2||