CLCN4 (NM_001830) Human Tagged ORF Clone

CAT#: RG217040

CLCN4 (tGFP-tagged) - Human chloride channel 4 (CLCN4)

Product Images

Circular map for RG217040

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CLCN4
• Synonyms: ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217040 representing NM_001830
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAATGCGGGAGCGATGAGTGGCTCTGGAAACCTGATGGATTTCCTCGATGAGCCGTTCCCTGATG
  TGGGGACGTATGAGGACTTCCACACCATCGACTGGCTAAGGGAAAAGTCACGGGACACCGACAGACACAG
  GAAGATCACCAGCAAGAGCAAGGAGTCCATATGGGAGTTCATCAAGAGCCTGCTGGATGCCTGGTCGGGA
  TGGGTGGTGATGCTGCTCATCGGCCTGCTGGCGGGCACCTTGGCTGGGGTCATCGATCTCGCCGTGGACT
  GGATGACGGACCTGAAGGAGGGGGTCTGCCTGTCTGCCTTCTGGTATAGCCATGAGCAGTGTTGCTGGAC
  TTCTAACGAGACCACTTTTGAGGACAGAGACAAGTGTCCCCTGTGGCAGAAATGGTCGGAGCTGCTGGTG
  AATCAGTCAGAGGGTGCCAGTGCTTACATTCTGAATTACTTAATGTACATCCTATGGGCGCTGCTGTTTG
  CATTTTTGGCTGTCTCCCTGGTGCGTGTATTTGCACCATATCGCTGTGGCTCTGGCATACCAGAGATAAA
  GACCATTTTGAGCGGCTTTATCATCAGGGGCTACTTGGGGAAGTGGACCCTGCTAATCAAGACAGTCACG
  CTGGTGCTGGTAGTGTCCTCCGGTCTGAGCCTTGGGAAGGAAGGGCCGCTAGTGCACGTGGCTTGTTGCT
  GTGGCAACTTCTTCAGCAGCCTTTTCTCCAAGTACAGCAAGAATGAGGGCAAGAGGCGGGAGGTGCTTTC
  AGCTGCAGCGGCTGCTGGAGTCTCTGTTGCCTTTGGTGCACCAATTGGAGGCGTGCTTTTCAGTCTAGAA
  GAGGTCAGTTACTACTTTCCCCTGAAGACCTTGTGGAGGTCATTTTTCGCAGCCCTGGTGGCGGCCTTTA
  CGCTGAGATCCATCAATCCCTTTGGGAATAGCCGTCTCGTTCTCTTTTATGTGGAATACCACACGCCCTG
  GTACATGGCTGAACTCTTCCCCTTCATCCTGCTTGGGGTCTTCGGGGGCTTGTGGGGAACCCTCTTCATC
  CGCTGCAACATCGCCTGGTGCAGGAGGCGCAAGACCACCAGGCTGGGGAAGTACCCGGTGCTGGAGGTCA
  TTGTGGTGACTGCCATCACTGCCATCATTGCCTACCCCAATCCCTACACACGCCAGAGCACCAGCGAGCT
  CATTTCTGAGCTGTTCAATGACTGTGGAGCCCTTGAGTCTTCCCAGCTCTGTGACTACATCAATGACCCC
  AACATGACTCGGCCTGTGGATGACATTCCAGACCGGCCGGCTGGTGTCGGTGTTTACACGGCCATGTGGC
  AGCTGGCCCTGGCACTGATCTTCAAAATCGTCGTTACCATATTTACCTTTGGCATGAAGATCCCGTCGGG
  CCTCTTCATCCCCAGCATGGCTGTGGGCGCGATAGCGGGCAGGATGGTGGGAATTGGCGTGGAGCAGCTG
  GCCTACCATCACCATGACTGGTACTACTTCAGGAACTGGTGCAGACCCGGTGCAGACTGTGTCACGCCAG
  GGCTGTACGCAATGGTGGGAGCTGCGGCCTGCCTCGGTGGAGTTACCAGGATGACGGTGTCATTGGTGGT
  CATCATGTTTGAATTAACCGGGGGTCTGGAGTACATCGTGCCCCTGATGGCGGCGGCTGTGACCAGCAAG
  TGGGTAGCTGATGCATTTGGGAAAGAAGGCATCTACGAGGCCCACATCCACTTAAATGGGTACCCTTTCC
  TTGACGTGAAGGACGAGTTTACTCACCGCACACTGGCCACCGACGTCATGCGGCCCCGGCGGGGAGAGCC
  GCCACTGTCGGTGCTCACCCAGGACAGCATGACTGTCGAGGACGTGGAGACGCTCATCAAGGAGACCGAC
  TACAACGGCTTCCCCGTGGTGGTCTCCAGAGACTCCGAGCGCCTCATTGGATTTGCCCAGAGGAGGGAAC
  TGATTCTCGCAATAAATAACGCCAGACAGAGGCAGGAGGGCATTGTGAGCAATTCCATCATGTACTTCAC
  GGAGGAACCCCCCGAGCTGCCGGCCAACAGCCCACATCCCCTGAAGCTGCGGCGCATCCTGAACCTCAGC
  CCGTTTACAGTGACAGACCACACTCCGATGGAAACGGTGGTGGATATCTTCCGGAAACTGGGGCTTCGGC
  AGTGCCTGGTGACGCGGAGCGGGAGACTTCTTGGCATCATCACAAAAAAGGATGTTCTGAGACATATGGC
  CCAGATGGCAAACCAGGACCCCGAATCCATCATGTTTAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217040 representing NM_001830
  Red=Cloning site Green=Tags(s)
  
  MVNAGAMSGSGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSG
  WVVMLLIGLLAGTLAGVIDLAVDWMTDLKEGVCLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLV
  NQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYRCGSGIPEIKTILSGFIIRGYLGKWTLLIKTVT
  LVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLE
  EVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFI
  RCNIAWCRRRKTTRLGKYPVLEVIVVTAITAIIAYPNPYTRQSTSELISELFNDCGALESSQLCDYINDP
  NMTRPVDDIPDRPAGVGVYTAMWQLALALIFKIVVTIFTFGMKIPSGLFIPSMAVGAIAGRMVGIGVEQL
  AYHHHDWYYFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSK
  WVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETD
  YNGFPVVVSRDSERLIGFAQRRELILAINNARQRQEGIVSNSIMYFTEEPPELPANSPHPLKLRRILNLS
  PFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001830
• ORF Size: 2280 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001830.2, NP_001821.1
• RefSeq Size: 4562 bp
• RefSeq ORF: 2283 bp
• Locus ID: 1183
• UniProt ID: P51793
• Cytogenetics: Xp22.2
• Domains: CBS, voltage_CLC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012]