NEDL2 (HECW2) (NM_020760) Human Tagged ORF Clone

CAT#: RG216544

HECW2 (tGFP-tagged) - Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2)

Product Images

Circular map for RG216544

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NEDL2
• Synonyms: NDHSAL; NEDL2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216544 representing NM_020760
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGTTCAGCCCGGGAGCACCTGCTTTTTGTGAGGCGTCGAAATCCCCAGATGCGGTACACATTGA
  GCCCAGAGAACCTCCAGAGCCTTGCCGCCCAGAGCTCCATGCCAGAGAACATGACCCTGCAGCGGGCCAA
  CAGCGACACCGACCTGGTGACTTCTGAGAGCCGCTCCAGCTTAACTGCCAGCATGTACGAGTACACGCTG
  GGGCAAGCCCAGAACCTCATTATCTTCTGGGACATTAAAGAGGAGGTGGACCCCAGTGATTGGATTGGAC
  TTTATCATATAGATGAGAATTCTCCAGCCAACTTCTGGGATTCTAAGAACAGGGGTGTGACTGGAACACA
  AAAAGGGCAAATTGTATGGAGAATTGAGCCTGGACCCTATTTCATGGAACCGGAGATAAAAATCTGTTTT
  AAATATTACCACGGCATTAGTGGAGCCCTGCGAGCCACGACCCCCTGCATCACCGTGAAGAACCCAGCTG
  TGATGATGGGGGCAGAAGGCATGGAGGGAGGTGCTTCAGGAAACCTGCATTCTCGAAAACTTGTTAGCTT
  TACATTGTCAGATCTTAGGGCAGTTGGGCTAAAGAAAGGGATGTTCTTCAATCCTGACCCTTATCTTAAG
  ATGTCAATTCAGCCAGGAAAGAAGAGCAGTTTCCCCACCTGTGCCCACCACGGGCAGGAGAGACGGTCTA
  CTATCATCAGTAACACCACCAATCCAATTTGGCACCGAGAGAAATATTCCTTTTTTGCACTTCTTACTGA
  TGTCTTAGAAATTGAAATTAAAGACAAATTTGCCAAGAGCCGTCCCATCATCAAGCGTTTTCTGGGGAAA
  CTAACCATTCCAGTCCAGAGGCTGCTGGAGCGACAAGCCATCGGTGATCAAATGCTCAGCTACAACCTTG
  GCAGAAGGCTCCCAGCTGACCACGTGAGTGGGTACCTCCAGTTTAAAGTGGAGGTTACGTCTTCTGTTCA
  TGAAGATGCCTCTCCAGAAGCTGTTGGCACAATACTTGGAGTCAATTCTGTGAATGGAGACTTAGGTAGC
  CCTTCCGATGACGAGGACATGCCAGGGAGCCATCACGACAGCCAGGTGTGCTCTAATGGGCCAGTTTCTG
  AGGACAGTGCTGCCGATGGAACCCCCAAGCATTCATTCAGGACTAGCTCCACTCTGGAAATAGACACAGA
  GGAATTAACCTCTACGTCTTCAAGGACCTCACCTCCCAGAGGACGTCAGGATTCACTCAATGATTACTTA
  GATGCTATCGAACACAATGGCCACTCCAGGCCAGGGACAGCGACCTGCTCTGAGAGGTCCATGGGTGCCT
  CTCCGAAACTGAGGAGTAGTTTTCCCACTGACACAAGACTCAATGCCATGCTTCATATTGATTCAGATGA
  AGAAGATCACGAGTTCCAGCAAGACCTGGGTTACCCATCTTCCTTGGAGGAGGAGGGAGGCCTGATCATG
  TTTAGCAGGGCATCCAGAGCTGATGATGGAAGCCTGACATCTCAGACAAAGCTGGAGGACAACCCTGTTG
  AGAATGAGGAAGCCTCCACACACGAAGCTGCTTCCTTTGAGGATAAGCCAGAAAATCTTCCAGAGCTTGC
  AGAGAGCTCCTTACCTGCAGGCCCAGCCCCAGAGGAAGGTGAAGGCGGCCCAGAGCCTCAACCCAGTGCT
  GACCAGGGCAGTGCTGAACTGTGTGGCTCTCAAGAGGTAGATCAGCCCACAAGTGGCGCAGACACAGGGA
  CTTCCGATGCATCAGGAGGAAGCCGAAGAGCCGTCAGTGAGACCGAGTCTCTCGATCAGGGCTCTGAGCC
  TTCCCAGGTGTCCTCTGAAACAGAACCCAGTGATCCTGCCAGGACAGAGAGTGTGAGCGAAGCCAGCACC
  AGGCCTGAGGGAGAGAGTGACCTGGAATGCGCTGACAGCTCCTGCAATGAGAGTGTGACCACGCAGCTGT
  CCTCTGTGGACACACGGTGCTCCTCTCTTGAGAGCGCACGGTTTCCTGAAACCCCAGCCTTTTCTTCTCA
  GGAGGAGGAAGACGGAGCCTGTGCAGCAGAGCCCACCAGCAGTGGCCCTGCCGAAGGGTCGCAGGAATCC
  GTGTGCACTGCTGGTTCTTTACCTGTGGTACAGGTGCCCAGTGGGGAGGATGAAGGGCCAGGGGCAGAAT
  CGGCCACTGTACCTGACCAGGAGGAGCTGGGGGAGGTCTGGCAGCGGAGGGGGAGCCTGGAGGGAGCTGC
  AGCTGCTGCCGAGAGCCCACCGCAAGAAGAAGGCAGTGCTGGGGAGGCCCAAGGCACCTGTGAAGGGGCA
  ACTGCCCAGGAGGAGGGCGCTACTGGAGGTTCTCAAGCCAACGGCCACCAGCCACTGCGATCACTACCTT
  CAGTGCGCCAGGATGTTAGCCGGTACCAGAGGGTGGACGAGGCTCTCCCACCAAACTGGGAGGCACGCAT
  TGACAGCCACGGCAGGATCTTCTACGTGGATCACGTAAACAGAACCACGACGTGGCAGCGACCGACAGCT
  CCCCCAGCCCCGCAGGTGCTGCAGAGATCTAACTCCATACAGCAAATGGAGCAGCTGAACCGGCGATATC
  AGAGTATCCGCAGAACCATGACCAATGAGAGGCCTGAGGAAAATACCAATGCTATTGATGGGGCAGGGGA
  GGAAGCTGACTTTCACCAAGCCAGTGCAGATTTCCGACGGGAGAACATCCTGCCTCACTCTACCTCCAGA
  TCCAGGATCACGTTGCTGTTACAGTCTCCCCCCGTGAAGTTCCTCATCAGCCCAGAGTTCTTCACCGTGC
  TGCATTCTAACCCTAGTGCCTACCGCATGTTTACAAACAACACGTGTTTGAAGCACATGATCACCAAAGT
  CCGGAGGGACACCCACCACTTTGAACGCTACCAGCATAACCGCGACCTTGTGGGATTCCTCAACATGTTC
  GCGAACAAACAGCTAGAGCTGCCGCGGGGATGGGAAATGAAACATGATCACCAGGGCAAGGCATTCTTTG
  TGGACCACAACTCCCGCACCACCACTTTCATTGATCCCCGGCTCCCACTTCAGAGCAGTAGACCCACAAG
  TGCGCTGGTTCATCGGCAACACCTGACAAGGCAACGCAGCCACAGTGCGGGTGAGGTAGGAGAAGATTCT
  CGACATGCAGGACCACCAGTTCTTCCCAGGCCATCCAGTACATTCAATACAGTCAGTAGGCCACAGTACC
  AGGACATGGTTCCAGTGGCTTACAATGACAAGATTGTTGCATTTCTACGTCAACCCAATATCTTTGAAAT
  TCTACAGGAGCGTCAACCTGATCTTACCAGGAATCACTCACTCAGGGAGAAGATCCAATTTATCCGAACT
  GAAGGGACTCCAGGATTGGTGCGCCTTTCAAGCGATGCAGACCTTGTTATGTTGCTGAGCTTATTTGAAG
  AAGAGATAATGTCATATGTGCCTCCACATGCCTTACTCCACCCCAGCTACTGTCAGTCCCCACGTGGCTC
  TCCCGTGTCATCTCCTCAGAACTCGCCAGGTACCCAGCGTGCCAATGCCCGGGCTCCAGCCCCTTACAAG
  CGGGATTTCGAAGCCAAACTGAGGAACTTTTACAGGAAGTTAGAGACTAAAGGATATGGACAAGGCCCAG
  GGAAGTTAAAGTTAATTATCCGAAGAGATCACTTACTAGAAGATGCTTTTAATCAGATTATGGGCTACTC
  CAGAAAAGACCTGCAGAGAAATAAGCTATATGTCACCTTCGTTGGGGAGGAAGGGCTGGATTACAGTGGG
  CCTTCTAGAGAGTTTTTCTTCCTGGTATCCAGAGAACTCTTTAACCCATATTATGGCTTATTTGAATATT
  CAGCCAATGACACATACACAGTACAAATAAGTCCTATGTCTGCTTTTGTAGACAATCACCATGAATGGTT
  CCGATTCAGTGGTAGGATCCTTGGTCTTGCACTAATACACCAGTATTTGTTGGATGCCTTCTTCACACGG
  CCCTTTTATAAGGCTCTTCTCAGAATTCTATGTGACCTGAGTGACCTAGAATACCTTGATGAAGAGTTCC
  ATCAGAGCCTGCAGTGGATGAAAGACAATGATATCCATGACATCCTAGACCTCACGTTCACTGTGAACGA
  AGAAGTATTTGGGCAGATAACTGAACGAGAATTAAAGCCAGGGGGTGCCAATATCCCAGTTACAGAGAAG
  AACAAGAAGGAGTACATCGAGAGGATGGTGAAGTGGAGGATTGAGAGGGGTGTTGTACAGCAAACAGAGA
  GCTTAGTGCGTGGCTTCTATGAGGTGGTGGATGCCAGGCTGGTATCTGTTTTTGATGCAAGAGAACTGGA
  ATTGGTCATCGCAGGCACAGCTGAAATAGACCTAAGTGATTGGAGAAACAACACAGAATATAGAGGAGGA
  TACCATGACAATCATATTGTAATTCGGTGGTTCTGGGCTGCAGTGGAAAGATTCAACAATGAACAACGAC
  TAAGGTTGTTACAGTTTGTTACAGGCACATCCAGCATTCCCTATGAAGGATTTGCTTCACTCCGAGGGAG
  TAACGGCCCAAGAAGATTCTGTGTGGAGAAATGGGGGAAAATCACTGCTCTTCCCAGAGCGCATACATGT
  TTTAACCGTCTGGATCTGCCTCCCTACCCATCCTTTTCCATGCTTTATGAAAAACTGTTGACAGCAGTTG
  AAGAAACCAGTACTTTTGGACTTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216544 representing NM_020760
  Red=Cloning site Green=Tags(s)
  
  MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTL
  GQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICF
  KYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLK
  MSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGK
  LTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSVNGDLGS
  PSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSFRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYL
  DAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIM
  FSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSA
  DQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEAST
  RPEGESDLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQES
  VCTAGSLPVVQVPSGEDEGPGAESATVPDQEELGEVWQRRGSLEGAAAAAESPPQEEGSAGEAQGTCEGA
  TAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTA
  PPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSR
  SRITLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMF
  ANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDS
  RHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRT
  EGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYK
  RDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSG
  PSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTR
  PFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEK
  NKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGG
  YHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTC
  FNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020760
• ORF Size: 4716 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020760.1, NP_065811.1
• RefSeq Size: 6926 bp
• RefSeq ORF: 4719 bp
• Locus ID: 57520
• UniProt ID: Q9P2P5
• Cytogenetics: 2q32.3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of a family of E3 ubiquitin ligases which plays an important role in the proliferation, migration and differentiation of neural crest cells as a regulator of glial cell line-derived neurotrophic factor (GDNF)/Ret signaling. This gene also plays an important role in angiogenesis through stabilization of endothelial cell-to-cell junctions as a regulator of angiomotin-like 1 stability. The encoded protein contains an N-terminal calcium/lipid-binding (C2) domain involved in membrane targeting, two-four WW domains responsible for cellular localization and substrate recognition, and a C-terminal homologous with E6-associated protein C-terminus (HECT) catalytic domain. Naturally occurring mutations in this gene are associated with neurodevelopmental delay, hypotonia, and epilepsy. The decreased expression of this gene in the aganglionic colon is associated with Hirschsprung's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]