CILK1 (NM_016513) Human Tagged ORF Clone

CAT#: RG216454

ICK (tGFP-tagged) - Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2

Product Images

Circular map for RG216454

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CILK1
• Synonyms: ECO; EJM10; hICK; ICK; LCK2; MRK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216454 representing NM_016513
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAGATACACAACAATCAGGCAGCTCGGGGATGGAACCTACGGTTCCGTCCTGCTGGGAAGAAGCA
  TTGAGTCTGGGGAGCTGATCGCTATTAAAAAAATGAAAAGAAAATTTTATTCCTGGGAGGAATGCATGAA
  CCTTCGGGAGGTTAAGTCTTTAAAGAAGCTCAACCATGCCAATGTAGTCAAATTAAAAGAAGTTATCAGG
  GAAAATGATCATCTTTATTTTATCTTCGAGTACATGAAGGAAAATCTTTACCAGCTCATTAAAGAGAGAA
  ATAAGTTGTTTCCTGAGTCTGCTATAAGGAATATCATGTATCAGATATTACAAGGACTCGCATTTATTCA
  CAAACACGGCTTCTTTCATCGAGACTTAAAGCCTGAGAACCTCCTCTGCATGGGACCAGAACTTGTGAAA
  ATTGCAGACTTTGGTTTGGCCCGAGAAATACGATCAAAACCTCCATATACAGATTATGTATCTACCAGAT
  GGTACAGGGCTCCAGAAGTACTCCTGAGGTCTACCAACTACAGCTCCCCCATTGACGTCTGGGCGGTGGG
  CTGCATCATGGCAGAAGTTTACACCCTCAGGCCACTCTTCCCTGGAGCCAGTGAAATTGACACAATATTC
  AAAATTTGCCAAGTGCTGGGGACACCAAAAAAGACTGACTGGCCTGAAGGCTATCAACTTTCAAGTGCAA
  TGAACTTCCGTTGGCCACAGTGTGTACCCAATAACTTAAAGACCTTGATTCCCAATGCTAGCAGTGAAGC
  AGTCCAGCTCCTGAGAGACATGCTTCAGTGGGATCCCAAGAAACGACCAACAGCTAGTCAGGCACTTCGA
  TATCCTTACTTCCAAGTTGGACACCCACTAGGCAGCACCACACAAAACCTTCAGGATTCAGAAAAACCAC
  AGAAAGGCATCCTGGAAAAGGCAGGCCCACCTCCTTATATTAAGCCAGTCCCACCTGCCCAGCCACCAGC
  CAAGCCACACACACGAATTTCTTCACGACAGCATCAAGCCAGCCAGCCCCCTCTGCATCTCACGTACCCC
  TACAAAGCAGAGGTCTCCAGGACAGATCACCCAAGCCATCTCCAGGAGGACAAGCCAAGCCCGTTGCTTT
  TCCCATCCCTCCACAACAAGCATCCACAGTCGAAAATCACAGCTGGCCTGGAGCACAAAAATGGTGAGAT
  AAAGCCAAAGAGTAGGAGAAGGTGGGGTCTTATTTCCAGGTCAACAAAGGATTCAGATGATTGGGCTGAC
  TTGGATGACTTGGATTTCAGTCCATCCCTCAGCAGGATTGACCTGAAAAACAAGAAAAGACAGAGTGATG
  ACACTCTCTGCAGGTTTGAGAGTGTTTTGGACCTGAAGCCCTCTGAGCCTGTGGGCACAGGAAACAGTGC
  CCCCACCCAGACGTCATATCAGCGGCGAGACACGCCCACCCTGAGATCTGCAGCCAAGCAGCACTATTTG
  AAGCACTCTCGATACTTGCCTGGGATCAGTATAAGAAATGGCATACTCTCGAATCCAGGCAAGGAATTTA
  TTCCACCTAATCCATGGTCTAGTTCTGGCTTGTCTGGAAAATCTTCAGGGACAATGTCAGTAATCAGCAA
  AGTAAATTCAGTTGGTTCCAGCTCTACAAGTTCTAGTGGACTGACTGGAAACTATGTCCCTTCCTTTCTG
  AAAAAAGAAATCGGTTCTGCTATGCAGAGGGTACACCTAGCACCTATTCCAGACCCTTCCCCTGGTTATT
  CCTCCCTGAAGGCCATGAGACCTCATCCTGGGCGACCATTCTTCCACACCCAGCCTAGAAGCACTCCTGG
  GTTGATACCACGGCCTCCAGCCGCCCAGCCAGTGCATGGCCGGACAGACTGGGCTTCCAAGTACGCATCT
  CGGCGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216454 representing NM_016513
  Red=Cloning site Green=Tags(s)
  
  MNRYTTIRQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANVVKLKEVIR
  ENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVK
  IADFGLAREIRSKPPYTDYVSTRWYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIF
  KICQVLGTPKKTDWPEGYQLSSAMNFRWPQCVPNNLKTLIPNASSEAVQLLRDMLQWDPKKRPTASQALR
  YPYFQVGHPLGSTTQNLQDSEKPQKGILEKAGPPPYIKPVPPAQPPAKPHTRISSRQHQASQPPLHLTYP
  YKAEVSRTDHPSHLQEDKPSPLLFPSLHNKHPQSKITAGLEHKNGEIKPKSRRRWGLISRSTKDSDDWAD
  LDDLDFSPSLSRIDLKNKKRQSDDTLCRFESVLDLKPSEPVGTGNSAPTQTSYQRRDTPTLRSAAKQHYL
  KHSRYLPGISIRNGILSNPGKEFIPPNPWSSSGLSGKSSGTMSVISKVNSVGSSSTSSSGLTGNYVPSFL
  KKEIGSAMQRVHLAPIPDPSPGYSSLKAMRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRTDWASKYAS
  RR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016513
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016513.5
• RefSeq Size: 6228 bp
• RefSeq ORF: 1899 bp
• Locus ID: 22858
• UniProt ID: Q9UPZ9
• Cytogenetics: 6p12.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008]