SLC6A11 (NM_014229) Human Tagged ORF Clone

CAT#: RG216392

SLC6A11 (tGFP-tagged) - Human solute carrier family 6 (neurotransmitter transporter, GABA), member 11 (SLC6A11)

Product Images

Circular map for RG216392

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC6A11
• Synonyms: GAT-3; GAT3; GAT4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216392 representing NM_014229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCGGAGAAGGCGCTGCCCCTGGGCAATGGGAAGGCTGCTGAGGAGGCGCGGGAGTCCGAGGCGC
  CGGGTGGCGGCTGCAGCAGCGGGGGCGCGGCGCCCGCGCGCCACCCGCGCGTCAAGCGCGACAAGGCGGT
  CCACGAGCGCGGCCACTGGAACAACAAGGTGGAGTTCGTGCTGAGCGTGGCCGGGGAGATCATTGGGCTG
  GGCAACGTGTGGCGCTTCCCCTACCTGTGCTACAAGAACGGAGGAGGGGCATTCCTGATTCCCTACGTGG
  TGTTTTTTATTTGCTGTGGAATTCCTGTTTTTTTCCTGGAGACAGCTCTGGGGCAGTTCACAAGTGAAGG
  TGGCATTACGTGTTGGAGGAAAGTTTGCCCTTTATTTGAAGGCATTGGCTATGCAACACAGGTGATTGAG
  GCCCATCTGAATGTGTACTACATCATCATCCTGGCATGGGCCATTTTTTACCTGAGCAACTGCTTCACTA
  CTGAGCTACCCTGGGCTACCTGTGGGCATGAGTGGAACACAGAGAATTGTGTGGAGTTCCAGAAACTGAA
  TGTGAGCAACTACAGCCATGTGTCTCTGCAGAATGCCACCTCCCCTGTCATGGAGTTTTGGGAGCACCGG
  GTCCTGGCCATCTCTGACGGGATCGAGCACATCGGGAACCTTCGCTGGGAGCTGGCCTTGTGTCTCTTGG
  CAGCCTGGACCATCTGTTACTTCTGTATCTGGAAGGGGACCAAGTCTACAGGAAAGGTTGTATACGTGAC
  TGCGACATTCCCCTACATCATGCTGCTGATCCTCCTGATACGAGGGGTCACGTTGCCCGGGGCCTCAGAG
  GGCATCAAGTTCTACTTGTACCCTGACCTCTCCCGGCTCTCCGACCCCCAGGTCTGGGTAGATGCTGGAA
  CGCAGATCTTTTTCTCCTATGCCATTTGCCTGGGCTGTCTGACCGCTCTGGGAAGTTATAACAATTATAA
  CAACAACTGCTACAGGGACTGCATCATGCTCTGTTGCCTGAACAGCGGCACCAGCTTCGTGGCTGGGTTT
  GCCATCTTCTCAGTCCTGGGTTTTATGGCGTACGAGCAGGGGGTACCCATTGCTGAGGTGGCAGAGTCAG
  GCCCCGGCCTGGCCTTTATTGCGTACCCCAAGGCGGTCACCATGATGCCTCTCTCCCCGCTGTGGGCCAC
  CTTGTTCTTCATGATGCTCATCTTCCTGGGCCTGGACAGCCAGTTTGTGTGTGTGGAAAGCCTGGTGACC
  GCCGTGGTGGACATGTACCCCAAGGTTTTCCGGAGGGGTTACCGGCGGGAGCTGCTCATCCTAGCCTTGT
  CTGTTATCTCCTATTTTCTGGGCCTCGTGATGTTAACAGAGGGTGGCATGTACATCTTCCAGCTCTTTGA
  CTCCTATGCCGCCAGTGGGATGTGCCTTCTCTTCGTGGCCATCTTTGAGTGCATCTGCATCGGCTGGGTG
  TATGGAAGCAACCGGTTCTATGATAACATTGAAGACATGATTGGCTACCGGCCACCGTCGCTCATTAAGT
  GGTGCTGGATGATCATGACCCCTGGGATCTGCGCGGGGATCTTCATCTTCTTCTTGATCAAGTACAAGCC
  ACTCAAGTACAACAACATCTACACCTACCCAGCCTGGGGCTATGGCATTGGCTGGCTCATGGCCCTGTCC
  TCCATGCTCTGCATCCCGCTCTGGATCTGCATCACAGTGTGGAAGACGGAGGGGACACTGCCCGAGAAAC
  TCCAGAAGTTGACGACCCCCAGCACAGATCTGAAAATGCGGGGCAAGCTTGGGGTGAGCCCACGGATGGT
  GACAGTTAATGACTGTGATGCCAAACTCAAGAGTGACGGGACCATCGCAGCCATCACAGAGAAGGAGACG
  CACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216392 representing NM_014229
  Red=Cloning site Green=Tags(s)
  
  MTAEKALPLGNGKAAEEARESEAPGGGCSSGGAAPARHPRVKRDKAVHERGHWNNKVEFVLSVAGEIIGL
  GNVWRFPYLCYKNGGGAFLIPYVVFFICCGIPVFFLETALGQFTSEGGITCWRKVCPLFEGIGYATQVIE
  AHLNVYYIIILAWAIFYLSNCFTTELPWATCGHEWNTENCVEFQKLNVSNYSHVSLQNATSPVMEFWEHR
  VLAISDGIEHIGNLRWELALCLLAAWTICYFCIWKGTKSTGKVVYVTATFPYIMLLILLIRGVTLPGASE
  GIKFYLYPDLSRLSDPQVWVDAGTQIFFSYAICLGCLTALGSYNNYNNNCYRDCIMLCCLNSGTSFVAGF
  AIFSVLGFMAYEQGVPIAEVAESGPGLAFIAYPKAVTMMPLSPLWATLFFMMLIFLGLDSQFVCVESLVT
  AVVDMYPKVFRRGYRRELLILALSVISYFLGLVMLTEGGMYIFQLFDSYAASGMCLLFVAIFECICIGWV
  YGSNRFYDNIEDMIGYRPPSLIKWCWMIMTPGICAGIFIFFLIKYKPLKYNNIYTYPAWGYGIGWLMALS
  SMLCIPLWICITVWKTEGTLPEKLQKLTTPSTDLKMRGKLGVSPRMVTVNDCDAKLKSDGTIAAITEKET
  HF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014229
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014229.3
• RefSeq Size: 1991 bp
• RefSeq ORF: 1899 bp
• Locus ID: 6538
• UniProt ID: P48066
• Cytogenetics: 3p25.3
• Domains: SNF
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The protein encoded by this gene is a sodium-dependent transporter that uptakes gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter, which ends the GABA neurotransmission. Defects in this gene may result in epilepsy, behavioral problems, or intellectual problems. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]