TRPM2 (NM_003307) Human Tagged ORF Clone

CAT#: RG216220

TRPM2 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant 1

Product Images

Circular map for RG216220

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRPM2
• Synonyms: EREG1; KNP3; LTrpC-2; LTRPC2; NUDT9H; NUDT9L1; TRPC7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216220 representing NM_003307
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCTCAGCCCTGAGGAAAGCTGGCTCGGAGCAGGAGGAGGGCTTTGAGGGACTGCCCAGAAGGG
  TCACTGACCTGGGGATGGTCTCCAATCTCCGGCGCAGCAACAGCAGCCTCTTCAAGAGCTGGAGGCTACA
  GTGCCCCTTCGGCAACAATGACAAGCAAGAAAGCCTCAGTTCGTGGATTCCTGAAAACATCAAGAAGAAA
  GAATGCGTGTATTTTGTGGAAAGTTCCAAACTGTCTGATGCTGGGAAGGTGGTGTGTCAGTGTGGCTACA
  CGCATGAGCAGCACTTGGAGGAGGCTACCAAGCCCCACACCTTCCAGGGCACACAGTGGGACCCAAAGAA
  ACATGTCCAGGAGATGCCAACCGATGCCTTTGGCGACATCGTCTTCACGGGCCTGAGCCAGAAGGTGAAA
  AAGTACGTCCGAGTCTCCCAGGACACGCCCTCCAGCGTGATCTACCACCTCATGACCCAGCACTGGGGGC
  TGGACGTCCCCAATCTCTTGATCTCGGTGACCGGGGGGGCCAAGAACTTCAACATGAAGCCGCGGCTGAA
  GAGCATTTTCCGCAGAGGCCTGGTCAAGGTGGCTCAGACCACAGGGGCCTGGATCATCACAGGGGGGTCC
  CACACCGGCGTCATGAAGCAGGTAGGCGAGGCGGTGCGGGACTTCAGCCTGAGCAGCAGCTACAAGGAAG
  GCGAGCTCATCACCATCGGAGTCGCCACCTGGGGCACTGTCCACCGCCGCGAGGGCCTGATCCATCCCAC
  GGGCAGCTTCCCCGCCGAGTACATACTGGATGAGGATGGCCAAGGGAACCTGACCTGCCTAGACAGCAAC
  CACTCTCACTTCATCCTCGTGGACGACGGGACCCACGGCCAGTACGGGGTGGAGATTCCTCTGAGGACCA
  GGCTGGAGAAGTTCATATCGGAGCAGACCAAGGAAAGAGGAGGTGTGGCCATCAAGATCCCCATCGTGTG
  CGTGGTGCTGGAGGGCGGCCCGGGCACGTTGTACACCATCGACAACGCCACCACCAACGGCACCCCCTGT
  GTGGTTGTGGAGGGCTCGGGCCGCGTGGCCGACGTCATTGCCCAGGTGGCCAACCTGCCTGTCTCGGACA
  TCACTATCTCCCTGATCCAGCAGAAACTGAGCGTGTTCTTCCAGGAGATGTTTGAGACCTTCACGGAAAG
  CAGGATTGTCGAGTGGACCAAAAAGATCCAAGATATCGTCCGGAGGCGGCAGCTGCTGACTGTCTTCCGG
  GAAGGCAAGGATGGTCAGCAGGACGTGGATGTGGCCATCTTGCAGGCCTTGCTGAAAGCCTCACGGAGCC
  AAGACCACTTTGGCCACGAGAACTGGGACCACCAGCTGAAACTGGCAGTGGCATGGAATCGCGTGGACAT
  TGCCCGCAGTGAGATCTTCATGGATGAGTGGCAGTGGAAGCCTTCAGATCTGCACCCCACGATGACAGCT
  GCACTCATCTCCAACAAGCCTGAGTTTGTGAAGCTCTTCCTGGAGAACGGGGTGCAGCTGAAGGAGTTTG
  TCACCTGGGACACCTTGCTCTACCTGTACGAGAACCTGGACCCCTCCTGCCTGTTCCACAGCAAGCTGCA
  GAAGGTGCTGGTGGAGGATCCCGAGCGCCCGGCTTGCGCGCCCGCGGCGCCCCGCCTGCAGATGCACCAC
  GTGGCCCAGGTGCTGCGGGAGCTGCTGGGGGACTTCACGCAGCCGCTTTATCCCCGGCCCCGGCACAACG
  ACCGGCTGCGGCTCCTGCTGCCCGTTCCCCACGTCAAGCTCAACGTGCAGGGAGTGAGCCTCCGGTCCCT
  CTACAAGCGTTCCTCAGGCCATGTGACCTTCACCATGGACCCCATCCGTGACCTTCTCATTTGGGCCATT
  GTCCAGAACCGTCGGGAGCTGGCAGGAATCATCTGGGCTCAGAGCCAGGACTGCATCGCAGCGGCCTTGG
  CCTGCAGCAAGATCCTGAAGGAACTGTCCAAGGAGGAGGAGGACACGGACAGCTCGGAGGAGATGCTGGC
  GCTGGCGGAGGAGTATGAGCACAGAGCCATCGGGGTCTTCACCGAGTGCTACCGGAAGGACGAAGAGAGA
  GCCCAGAAACTGCTCACCCGCGTGTCCGAGGCCTGGGGGAAGACCACCTGCCTGCAGCTCGCCCTGGAGG
  CCAAGGACATGAAGTTTGTGTCTCACGGGGGCATCCAGGCCTTCCTGACCAAGGTGTGGTGGGGCCAGCT
  CTCCGTGGACAATGGGCTGTGGCGTGTGACCCTGTGCATGCTGGCCTTCCCGCTGCTCCTCACCGGCCTC
  ATCTCCTTCAGGGAGAAGAGGCTGCAGGATGTGGGCACCCCCGCGGCCCGCGCCCGTGCCTTCTTCACCG
  CACCCGTGGTGGTCTTCCACCTGAACATCCTCTCCTACTTCGCCTTCCTCTGCCTGTTCGCCTACGTGCT
  CATGGTGGACTTCCAGCCTGTGCCCTCCTGGTGCGAGTGTGCCATCTACCTCTGGCTCTTCTCCTTGGTG
  TGCGAGGAGATGCGGCAGCTCTTCTATGACCCTGACGAGTGCGGGCTGATGAAGAAGGCAGCCTTGTACT
  TCAGTGACTTCTGGAATAAGCTGGACGTCGGCGCAATCTTGCTCTTCGTGGCAGGGCTGACCTGCAGGCT
  CATCCCGGCGACGCTGTACCCCGGGCGCGTCATCCTCTCTCTGGACTTCATCCTGTTCTGCCTCCGGCTC
  ATGCACATTTTTACCATCAGTAAGACGCTGGGGCCCAAGATCATCATTGTGAAGCGGATGATGAAGGACG
  TCTTCTTCTTCCTCTTCCTGCTGGCTGTGTGGGTGGTGTCCTTCGGGGTGGCCAAGCAGGCCATCCTCAT
  CCACAACGAGCGCCGGGTGGACTGGCTGTTCCGAGGGGCCGTCTACCACTCCTACCTCACCATCTTCGGG
  CAGATCCCGGGCTACATCGACGGTGTGAACTTCAACCCGGAGCACTGCAGCCCCAATGGCACCGACCCCT
  ACAAGCCTAAGTGCCCCGAGAGCGACGCGACGCAGCAGAGGCCGGCCTTCCCTGAGTGGCTGACGGTCCT
  CCTACTCTGCCTCTACCTGCTCTTCACCAACATCCTGCTGCTCAACCTCCTCATCGCCATGTTCAACTAC
  ACCTTCCAGCAGGTGCAGGAGCACACGGACCAGATTTGGAAGTTCCAGCGCCATGACCTGATCGAGGAGT
  ACCACGGCCGCCCCGCCGCGCCGCCCCCCTTCATCCTCCTCAGCCACCTGCAGCTCTTCATCAAGAGGGT
  GGTCCTGAAGACTCCGGCCAAGAGGCACAAGCAGCTCAAGAACAAGCTGGAGAAGAACGAGGAGGCGGCC
  CTGCTATCCTGGGAGATCTACCTGAAGGAGAACTACCTCCAGAACCGACAGTTCCAGCAAAAGCAGCGGC
  CCGAGCAGAAGATCGAGGACATCAGCAATAAGGTTGACGCCATGGTGGACCTGCTGGACCTGGACCCACT
  GAAGAGGTCGGGCTCCATGGAGCAGAGGTTGGCCTCCCTGGAGGAGCAGGTGGCCCAGACAGCCCGAGCC
  CTGCACTGGATCGTGAGGACGCTGCGGGCCAGCGGCTTCAGCTCGGAGGCGGACGTCCCCACTCTGGCCT
  CCCAGAAGGCCGCGGAGGAGCCGGATGCTGAGCCGGGAGGCAGGAAGAAGACGGAGGAGCCGGGCGACAG
  CTACCACGTGAATGCCCGGCACCTCCTCTACCCCAACTGCCCTGTCACGCGCTTCCCCGTGCCCAACGAG
  AAGGTGCCCTGGGAGACGGAGTTCCTGATCTATGACCCACCCTTTTACACGGCAGAGAGGAAGGACGCGG
  CCGCCATGGACCCCATGGGAGACACCCTGGAGCCACTGTCCACGATCCAGTACAACGTGGTGGATGGCCT
  GAGGGACCGCCGGAGCTTCCACGGGCCGTACACAGTGCAGGCCGGGTTGCCCCTGAACCCCATGGGCCGC
  ACAGGACTGCGTGGGCGCGGGAGCCTCAGCTGCTTCGGACCCAACCACACGCTGTACCCCATGGTCACGC
  GGTGGAGGCGGAACGAGGATGGAGCCATCTGCAGGAAGAGCATAAAGAAGATGCTGGAAGTGCTGGTGGT
  GAAGCTCCCTCTCTCCGAGCACTGGGCCCTGCCTGGGGGCTCCCGGGAGCCAGGGGAGATGCTACCTCGG
  AAGCTGAAGCGGATCCTCCGGCAGGAGCACTGGCCGTCTTTTGAAAACTTGCTGAAGTGCGGCATGGAGG
  TGTACAAAGGCTACATGGATGACCCGAGGAACACGGACAATGCCTGGATCGAGACGGTGGCCGTCAGCGT
  CCACTTCCAGGACCAGAATGACGTGGAGCTGAACAGGCTGAACTCTAACCTGCACGCCTGCGACTCGGGG
  GCCTCCATCCGATGGCAGGTGGTGGACAGGCGCATCCCACTCTATGCGAACCACAAGACCCTCCTCCAGA
  AGGCAGCCGCTGAGTTCGGGGCTCACTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216220 representing NM_003307
  Red=Cloning site Green=Tags(s)
  
  MEPSALRKAGSEQEEGFEGLPRRVTDLGMVSNLRRSNSSLFKSWRLQCPFGNNDKQESLSSWIPENIKKK
  ECVYFVESSKLSDAGKVVCQCGYTHEQHLEEATKPHTFQGTQWDPKKHVQEMPTDAFGDIVFTGLSQKVK
  KYVRVSQDTPSSVIYHLMTQHWGLDVPNLLISVTGGAKNFNMKPRLKSIFRRGLVKVAQTTGAWIITGGS
  HTGVMKQVGEAVRDFSLSSSYKEGELITIGVATWGTVHRREGLIHPTGSFPAEYILDEDGQGNLTCLDSN
  HSHFILVDDGTHGQYGVEIPLRTRLEKFISEQTKERGGVAIKIPIVCVVLEGGPGTLYTIDNATTNGTPC
  VVVEGSGRVADVIAQVANLPVSDITISLIQQKLSVFFQEMFETFTESRIVEWTKKIQDIVRRRQLLTVFR
  EGKDGQQDVDVAILQALLKASRSQDHFGHENWDHQLKLAVAWNRVDIARSEIFMDEWQWKPSDLHPTMTA
  ALISNKPEFVKLFLENGVQLKEFVTWDTLLYLYENLDPSCLFHSKLQKVLVEDPERPACAPAAPRLQMHH
  VAQVLRELLGDFTQPLYPRPRHNDRLRLLLPVPHVKLNVQGVSLRSLYKRSSGHVTFTMDPIRDLLIWAI
  VQNRRELAGIIWAQSQDCIAAALACSKILKELSKEEEDTDSSEEMLALAEEYEHRAIGVFTECYRKDEER
  AQKLLTRVSEAWGKTTCLQLALEAKDMKFVSHGGIQAFLTKVWWGQLSVDNGLWRVTLCMLAFPLLLTGL
  ISFREKRLQDVGTPAARARAFFTAPVVVFHLNILSYFAFLCLFAYVLMVDFQPVPSWCECAIYLWLFSLV
  CEEMRQLFYDPDECGLMKKAALYFSDFWNKLDVGAILLFVAGLTCRLIPATLYPGRVILSLDFILFCLRL
  MHIFTISKTLGPKIIIVKRMMKDVFFFLFLLAVWVVSFGVAKQAILIHNERRVDWLFRGAVYHSYLTIFG
  QIPGYIDGVNFNPEHCSPNGTDPYKPKCPESDATQQRPAFPEWLTVLLLCLYLLFTNILLLNLLIAMFNY
  TFQQVQEHTDQIWKFQRHDLIEEYHGRPAAPPPFILLSHLQLFIKRVVLKTPAKRHKQLKNKLEKNEEAA
  LLSWEIYLKENYLQNRQFQQKQRPEQKIEDISNKVDAMVDLLDLDPLKRSGSMEQRLASLEEQVAQTARA
  LHWIVRTLRASGFSSEADVPTLASQKAAEEPDAEPGGRKKTEEPGDSYHVNARHLLYPNCPVTRFPVPNE
  KVPWETEFLIYDPPFYTAERKDAAAMDPMGDTLEPLSTIQYNVVDGLRDRRSFHGPYTVQAGLPLNPMGR
  TGLRGRGSLSCFGPNHTLYPMVTRWRRNEDGAICRKSIKKMLEVLVVKLPLSEHWALPGGSREPGEMLPR
  KLKRILRQEHWPSFENLLKCGMEVYKGYMDDPRNTDNAWIETVAVSVHFQDQNDVELNRLNSNLHACDSG
  ASIRWQVVDRRIPLYANHKTLLQKAAAEFGAHY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003307
• ORF Size: 4509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003307.3, NP_003298.1
• RefSeq Size: 5876 bp
• RefSeq ORF: 4512 bp
• Locus ID: 7226
• UniProt ID: O94759
• Cytogenetics: 21q22.3
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: The protein encoded by this gene forms a tetrameric cation channel that is permeable to calcium, sodium, and potassium and is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. Additional transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2016]